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Letter
| Planta Med 2005; 71: 77-79
DOI: 10.1055/s-2005-837755 |
© Georg Thieme Verlag KG Stuttgart · New York |
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DNA Topoisomerase I Inhibitor, Ergosterol Peroxide from Penicillium oxalicum |
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| Li-Ming Yang Kuo1, Kuei-Yuh Chen4, Shy-Yuan Hwang2, Jin-Liang Chen3, Yen-Yin Liu4, Chia-Ching Liaw1, Po-Ho Ye5, Cheng-Jen Chou1, Chien-Chang Shen1, Yao-Haur Kuo1 |
1 National Research Institute of Chinese Medicine, Taipei, Taiwan, R.O.C.
2 Department of Agriculture and Forestry, Taiwan Endemic Species Research Institute, Nantou County, Taiwan, R.O.C.,
3 Department of Hospital and Health Care Administration, Chia Nan University of Pharmacy and Science, Tainan, Taiwan, R.O.C.
4 Department of Biology, Chinese Culture University, Taipei, Taiwan, R.O.C.
5 Institute of Pharmacology, National Yang Ming University, Taipei, Taiwan, R.O.C. |
Abstract
Recently, we found that the MeOH extract of Penicillium oxalicum showed inhibitory activity towards DNA topoisomerase I. Subsequently, ergosterol peroxide, ergosterol, palmitoleic acid, and linoleic acid were isolated from the cultured mycelia of P. oxalicum. The structural determinations were based on physical and spectral analyses. Biological evaluation revealed that ergosterol peroxide inhibited the relaxation of supercoiled DNA (pBR322) induced by DNA topoisomerase I, and also showed marginal, selective cytotoxic activity against human colon tumor cells [COLO-205 (ED50) = 8.56 μg/mL].
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