Original Paper Analytical Methods	Planta Med 2005; 71: 1152-1156 DOI: 10.1055/s-2005-873151
© Georg Thieme Verlag KG Stuttgart · New York
Determination of Five Coumarins in Cnidii Fructus by Micellar Electrokinetic Capillary Chromatography
Meng Hua Wu1, Lu Hua Zhao1, Yue Song1, Wei Zhang2, Bing Ren Xiang1, Ling Hua Mei3
1 Key Laboratory of Modern Chinese Medicine, Ministry of Education, Nanjing, P.R. China 2 Guangzhou Institute for Drug Control, Guangzhou, P.R. China 3 Jiangxi Huiren Pharmaceutical Co., Ltd., Nanchang, P.R. China

Abstract

A micellar electrokinetic capillary chromatography (MEKC) method for the separation and quantification of the coumarins in Cnidii Fructus was developed for the first time. Within 25 minutes, 5 major coumarins were separated using 18 mM borate, 12 mM phosphate and 50 mM SDS (pH 9.2) containing 20 % methanol. The relative standard deviations of migration times and peak areas were less than 5 %. The total contents of the five coumarins in plants from different habitats ranged from 13.6 to 44.6 mg/g, and recoveries ranged from 96.0 to 102.8 %. The effects of buffer concentration, SDS, pH value and organic modifier on the separation were investigated.

Key words

Capillary electrophoresis - micellar electrokinetic capillary chromatography - coumarins - Cnidium monnieri - Umbelliferae