Original Basic	Horm Metab Res 2006; 38: 631-638 DOI: 10.1055/s-2006-954580
© Georg Thieme Verlag KG Stuttgart · New York
Subcutaneous Fat Modulates Insulin Sensitivity in Mice by Regulating TNF-α Expression in Visceral Fat
K. Ishikawa1, K. Takahashi1, H. Bujo2, N. Hashimoto3, K. Yagui1, Y. Saito1
1Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan 2Department of Genome Research and Clinical Application, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan 3Division of Applied Translational Research, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan

Abstract

The distribution of fat in obese persons is related to the risk of developing various metabolic disorders, such as glucose intolerance, dyslipidemia and hypertension, and the combination of these conditions is known as the metabolic syndrome. The aim of this study was to investigate the role of subcutaneous fat in regulating insulin resistance and its influence on TNF-α expression in visceral fat, by using mice that were subjected to subcutaneous lipectomy with or without subsequent fat transplantation. After partial subcutaneous lipectomy, mice showed significantly greater accumulation of visceral fat compared with sham-operated control mice. Lipectomy led to higher plasma insulin and lower plasma glucose levels after loading with glucose and insulin, respectively, compared with the levels in control mice. Insulin-induced phosphorylation of IRS-1 was decreased in the skeletal muscles of lipectomized mice. Subcutaneous transplantation of fat pads into lipectomized mice reversed the above-mentioned changes indicating insulin resistance in these animals. The fat storage area of adipocytes and TNF- α expression by adipocytes in visceral fat were significantly higher in the lipectomized mice than in controls, while subcutaneous transplantation of fat reduced both the fat storage area and TNF-α expression. The insulin resistance of lipectomized mice was also ameliorated by systemic neutralization of TNF-α activity using a specific antibody. These findings obtained in mice subjected to subcutaneous lipectomy with/without subsequent fat transplantation indicate that subcutaneous fat regulates systemic insulin sensitivity, possibly through altering fat storage and the expression of TNF-α by adipocytes in visceral fat. The balance between accumulation of subcutaneous fat and visceral fat may be important with respect to the occurrence of systemic insulin resistance in the metabolic syndrome.

Key words

Lipectomy - TNF-α - subcutaneous - visceral - insulin resistance