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Analytical Methods
Original Paper | Planta Med 2007; 73: 1225-1229
DOI: 10.1055/s-2007-981590 |
© Georg Thieme Verlag KG Stuttgart · New York |
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Investigation of the Hydrolysis of Ginsenosides by High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry |
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| Xu Zhang1, 2, Fengrui Song1, Meng Cui1, Zhiqiang Liu1, Shuying Liu1 |
1 Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun Center of Mass Spectrometry, Changchun, P. R. China
2 Graduate University of the Chinese Academy of Sciences, Beijing, P. R. China |
Abstract
The hydrolysis of ginsenoside standards and the crude extracts of ginseng has been investigated at different pH values (2.4 - 11.2) using high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS). The experimental results indicated that the pH value of aqueous solutions is an important factor in changing the composition of ginsenosides. For (20S)-protopanaxadiol ginsenosides, ginsenosides with a large mass hydrolyzed to form hydrolysates (20S)-Rg3 and (20R)-Rg3 at pH 4.3. There were more hydrolyzed products observed at pH 3.3: (20S)-F2, C-25,26 hydrated ginsenoside ”C-Y1” and ”C-Y2” (MW = 802 Da) accompanied with (20S)-Rg3, (20R)-Rg3. At pH 2.4, only (20R)-Rg3, (20S)-F2, a small quantity of (20S)-Rg3 and three C-25,26 hydrated ginsenosides were obtained. For (20S)-protopanaxatriol Re, no hydrolysates were observed at pH 4.3; it was hydrolyzed at pH 3.3 to form hydrolysates (20S)-Rg2, (20R)-Rg2 and hydrated C-25,26 (MW = 802 Da) and at pH 2.4 only C-25,26 hydrated ginsenosides ”C-Y1” and ”C-Y2” (MW = 802 Da) were left in the solution. Similar hydrolysis reactions could be also observed for the crude extracts of ginseng. It showed that HPLC/ESI-MS is a fast and convenient method to study the hydrolysis of ginseng.
Key words
Hydrolysis - ginseng - Panax ginseng - Panax quinquefolius - Araliaceae - ginsenoside - HPLC-ESI-MS
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