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Horm Metab Res 1975; 7(4): 309-314
DOI: 10.1055/s-0028-1093720
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© Georg Thieme Verlag KG Stuttgart · New York

Existence in Fetal and Adult Rat of Several Forms of Material Reacting with Anti-Insulin Antibody (IRI)[*]

J. M. Felix , M.-Th.  Sutter-Dub , C.  Legrele , B.  Billaudel
  • Laboratoire de Physiologie animale et Centre de Biologie et de Biochimie du Développement, Faculté des Sciences, Reims, France
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Publikationsdatum:
23. Dezember 2008 (online)

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Abstract

The double antibody procedure detects 3 peaks in the elution fractions of adult or fetal rat sera, after passage on Sephadex G50 or G100 columns. Peak A (apparent MW 6000) contains insulin monomer; peak B (apparent MW 10-12000) is tentatively attributed to pro insulin (or proinsulin like substances); peak C (apparent MW 50-100000) is similar to the so called "big big" insulin. During intravenously induced hyperglycemia, the 3 peaks show parallel increases, but, after the disappearance of peaks A and B in streptozotocin treated rats, peak C remains unaltered. Pancreatic extracts and secreta present a very minor and inconstant peak C, the bulk of their immunoreactive material belonging to peaks A and B. A companion paper further discusses the nature of peaks B and C materials.

Key words

Circulating Insulin - IRI Big Insulin - Fetus - Adult Rat

1 Supported by ATP n° 429912 and by D.G.R.S.T. grant n° 71-7-3162

1 Supported by ATP n° 429912 and by D.G.R.S.T. grant n° 71-7-3162

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