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Thromb Haemost 1988; 60(03): 463-467
DOI: 10.1055/s-0038-1646991
Original Article
Schattauer GmbH Stuttgart

Clot Lysis Mediated by Cultured Human Microvascular Endothelial Cells

Wolfgang Speiser
The Clinical Research Unit for Blood Coagulation and Thrombosis of the Max-Planck-Gesellschaft, Justus-Liebig-Universität, Giessen, FRG
,
Elisabeth Anders
The Clinical Research Unit for Blood Coagulation and Thrombosis of the Max-Planck-Gesellschaft, Justus-Liebig-Universität, Giessen, FRG
,
Bernd R Binder
*   The Laboratory for Clinical and Experimental Physiology, Department of Medical Physiology, University of Vienna, Austria
,
Gert Müller-Berghaus
The Clinical Research Unit for Blood Coagulation and Thrombosis of the Max-Planck-Gesellschaft, Justus-Liebig-Universität, Giessen, FRG
› Author Affiliations
Further Information

Publication History

Received 16 February 1988

Accepted after revision 05 August 1988

Publication Date:
30 June 2018 (online)

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Summary

The lysis of fibrin clots on the surface of cultured human omental tissue microvascular endothelial cells (HOTMEC) and cultured human umbilical vein endothelial cells (HUVEC) was studied. Fibrin clots were made by mixing fibrinogen, plasminogen and thrombin on the surface of both cell types. Clot lysis was seen only on the surface of HOTMEC, which were found to synthesize about 100-fold more tissue plasminogen activator (tPA) antigen than HUVEC. Clot lysis of HOTMEC could be blocked by anti-tPA IgG but was not affected by the incorporation of exogenous plasminogen activator (PAI) into the clot in concentrations (75 arbitrary units) exceeding the tPA activity (21 ± 2.5 IU) of the cells. Thus, it is likely that tPA secreted by HOTMEC is protected from inhibition by PAI in the presence of fibrin and endothelial cells. The stimulation of EC to release an excess of tPA over PAI, in contrast to the secretion of an excess of PAI over tPA found in unstimulated cells in the absence of fibrin, is obviously no prerequisite for the initiation of fibrinolysis on the surface of HOTMEC. As thrombin was used for clot formation, its influence on tPA and PAI synthesis of both cell types was investigated. In contrast to HOTMEC, which were not affected by Α-thrombin, HUVEC revealed a dose-dependent increase in tPA and PAI synthesis upon incubation with the enzyme. This increase in tPA production by HUVEC was not sufficient to lyse the clots within 48 hours. Furthermore, HUVEC. behaved differently towards thrombin as these cells in contrast to HOTMEC revealed the typical shape change reaction upon incubation with the enzyme

Keywords

Endothelial cells - Clot lysis - Tissue plasminogen activator - Plasminogen activator inhibitor
 

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