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Thromb Haemost 1984; 52(03): 256-262
DOI: 10.1055/s-0038-1661191
Original Article
Schattauer GmbH Stuttgart

Studies on Prothrombin Complex Concentrates Contact Factors, Complement Components and Proteinase Inhibitors

M Steinbuch
The Laboratoire de Biochimie, C.N.T.S., Paris, France
,
L Péjaudier
The Laboratoire de Biochimie, C.N.T.S., Paris, France
,
V Kichenin
The Laboratoire de Biochimie, C.N.T.S., Paris, France
,
M C Boffa
*   The les Ulis et Laboratoire de Recherche en Hémostase et Thrombose, C.N.T.S., Paris, France
› Author Affiliations
Further Information

Publication History

Received 02 July 1984

Accepted 17 August 1984

Publication Date:
19 July 2018 (online)

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Summary

The behaviour of contact factors, complement components and antiproteases during the preparation of prothrombin complex concentrates by adsorption of the clotting components on DEAE-Sephadex has been studied.

The pro-enzymes: factors XII, XI and prekallikrein were removed by pre-elution in function of the salt concentration. In contrast, high molecular weight kininogen was considerably enriched in PCC preparations. C4 of the complement system displayed an analogous behaviour. Cls reached a 4-5 fold plasma concentration but C3 only 30% of the normal plasma level.

The prothrombin complex concentrate contained no antithrombin III nor α2M nor α2 antiplasmin but a three fold plasma concentration of Cl-inactivator and a 15 fold increase of inter-α- trypsin inhibitor.

NAPTT (Non Activated Partial Thromboplastin Time) ratios did not seem to be in accordance with either the presence or the absence of contact enzymes. Moreover 0.20 M NaCl appeared as the minimal pre-elution molarity necessary to ensure a NAPTT ratio above thrombogenic values.

Molecular alteration of high molecular weight kininogen and C4 was observed and its significance discussed. Complex formation between C1-inactivator and proteases was shown to be another sign of undesirable proteolytic events.

Key words

Prothrombin complex - Contact factors - Complement components - Proteinase inhibitors
 

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