Expression and function of histone deacetylase 7 in hepatocellular carcinoma

Introduction An imbalance between the acetylation and deacetylation of histone proteins regulated by histone deacetylases (HDACs) has been shown in different types of cancer including hepatocellular carcinoma (HCC). HDAC inhibition appears as promising therapeutic strategy for HCC. However, mostly unspecific HDAC inhibitiors have been applied and the knowledge about the expression and role of individual HDACs in HCC is very limited.

The aim of the study was to unveil the expression and function of HDAC7 in HCC.

Methods and Results HDAC7 expression was significantly increased in 4 human HCC cell lines (Hep3B, HepG2, PLC, Huh7) compared to primary human hepatocytes (PHH). Furthermore, HDAC7 expression in a spontaneous murine HCC model and human HCC specimens compared to non-tumorous liver tissue were significantly increased. TCGA (The Cancer Genome Atlas) data set analysis revealed a significant correlation between high HDAC7 expression and poor patients’ survival. Interestingly, in silico analysis applying the GEPIA-platform showed that HDAC7 expression in human HCC tissues correlates significantly with the expression of genes regulated by hypoxia such as GLUT1 and VEGF. In line with this, hypoxia significantly increased HDAC7 expression in human HCC cells in vitro. Further in vitro studies showed that siRNA mediated depletion of HDAC7 significantly reduced NFkB-activity in HCC cells. Furthermore, we found increased p62 and LC3 II protein levels in HDAC7-depleted cells indicating an impairment of the autophagic flux.

Summary and Conclusion Our study indicates HDAC7 as strong pro-tumorigenic factor in HCC and newly identifies hypoxia, a condition frequently occurring in HCC, as promotor of HDAC7 expression in HCC. These data indicate HDAC7 expression as prognostic marker and suggest specific HDAC7 inhibition as promising therapeutic strategy in HCC.

Publikationsverlauf

Artikel online veröffentlicht:
04. Januar 2021