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Horm Metab Res 1993; 25(7): 344-347
DOI: 10.1055/s-2007-1002116
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© Georg Thieme Verlag, Stuttgart · New York

Induction of Islet Cell Surface and Insulin Antibodies in Balb/c Mice by Application of Porcine Insulin and Freund's Adjuvant is not Associated with Insulitis

Petra Augstein, K.-D. Kohnert, Brigitte Ziegler, Birgitt Fürll, P. Heinke, M. Ziegler
  • Institute of Diabetes Research “Gerhardt Katsch”, Karlsburg, University of Greifswald, Greifswald, Germany
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Publikationsverlauf

1992

1993

Publikationsdatum:
14. März 2008 (online)

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Summary

To investigate whether insulin antibody (IAB) formation is associated with the appearance of islet cell cytoplasmic antibodies (ICA), islet cell surface antibodies (ICSA) and insulitis Balb/c Bln mice were immunized with porcine insulin in combination with or without Freund's adjuvant. The animals received 8 i.p. injections and were followed up to 150 days for the development of antibodies and insulitis. Mice immunized with insulin in CFA developed IAB as well as ICSA. Mice only receiving Freund's adjuvant emulsified in saline also developed ICSA. ICA were not detectable. Inflammatory infiltrates were found in the exocrine pancreatic parenchyma but not in islets. The results show that nonspecific stimulation of the immune system and the application of insulin as antigen leads to both the formation of ICSA and IAB, while insulitis was not detectable.

Key words

ICSA - ICA - Insulitis - Porcine Insulin - Balb/c Mice

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