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Horm Metab Res 1989; 21(9): 489-493
DOI: 10.1055/s-2007-1009269
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© Georg Thieme Verlag, Stuttgart · New York

Actinomycin D Inhibits the Rapid Increase in Translatable Calcitonin mRNA Provoked by Acute Calcium Stimulation

N. Segond, M. C. Delehaye, A. Jullienne, J. Taboulet, S. Minvielle, G. Milhaud, M. S. Moukhtar
  • Laboratoire d'Endocrinologie Moléculaire, U. 113 INSERM et UA 163 CNRS, CHU St. Antoine, Paris, France
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Publikationsverlauf

1986

1988

Publikationsdatum:
14. März 2008 (online)

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Summary

Calcium, injected to rats, elicits a rapid increase in translatable calcitonin mRNA, by acting probably at the post-transcriptional level, as no change in calcitonin mRNA could be detected by hybridization assay. In this study we have measured calcitonin mRNA extracted from rats subjected or not to acute hypercalcemia and pretreated or not with actinomycin D. Calcitonin mRNA was quantified by its ability to direct the synthesis of calcitonin (CT) precursors in a cell free system and by hybridization to a 32P cDNA probe specific for CT mRNA. Actinomycin D, injected 5 hours before calcium administration, decreased the incorporation of 3H adenine in liver and thyroid, but did not inhibit the rise in plasma levels of calcium and CT (measured by radioimmunoassay). The antibiotic was able to inhibit the eightfold increase in translatable mRNA elicited by calcium administration in the control animals. Hybridizable CT mRNA levels were not modified by the treatments. Thus the increase in translatable CT mRNA after calcium stimulation is independent of CT secretion and is probably due to post-transcriptional modifications involving the expression of other gene(s).

Key-Words

Regulation - Post-Transcriptional - Transcription - Gene(s) - Secretion

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