Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596921
Abstracts
Georg Thieme Verlag KG Stuttgart · New York

Dietary supplementation with sulfated galactans from Gracilaria fisheri enhances immunity and protects against Vibrio parahaemolyticus infection in shrimp

Authors

  • T Rudtanatip

    1   Department of Anatomy

  • N Boonsri

    1   Department of Anatomy

  • B Withyachumnarnkul

    1   Department of Anatomy
    2   Centex Shrimp, Faculty of Science, Mahidol University, Rama 6th Road, 10400 Bangkok, Thailand
    3   The Shrimp Genetic Improvement Center, Chaiya District, 84100 Surat Thani, Thailand

  • K Wongprasert

    1   Department of Anatomy
Further Information

Publication History

Publication Date:
14 December 2016 (online)

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Natural immunostimulant agents have recently received much attention due to their benefits in disease control. Our previous studies demonstrated that sulfated galactans (SG) from Gracilaria fisheri (G. fisheri) exhibited immunostimulant and antiviral activities in shrimp [1]. Moreover, we have reported that SG from G. fisheri stimulated expression of immune-related genes in shrimp haemocyte culture through the LGBP, and IMD-NF-κB pathway [2]. The present study further investigated the immunostimulating and protective effects of SG supplemented diets against Vibrio parahaemolyticus (V. parahaemolyticus) infection in shrimp. Shrimp were fed with SG supplemented diets for 7 days, and the immune-related genes were analyzed. The results revealed that SG supplemented diet enhanced expression of immune-related genes including IMD, IKKβ, IKKε, p-NF-κB, PEN-4, Dicer, proPO-I and -II in dose-dependent fashion. After feeding with SG supplemented diet for 7 days, shrimp were challenged with V. parahaemolyticus, and the cumulative mortality rate was observed for 14 days. The result revealed that SG supplemented diet reduced the shrimp mortality from V. parahaemolyticus infection compared to control. Furthermore, signs of V. parahaemolyticus infection including histopathological feature, number of bacterial colonies and expression of the bacterial toxin (AP3) mRNA transcript of V. parahaemolyticus significantly decreased from the control. This finding suggested that SG supplemented diet successfully enhanced expression of immune-related genes and protected the shrimp from V. parahaemolyticus infection.

Acknowledgements: This research was supported by the Thailand Research Fund (TRF) through the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0148/2554).

Keywords: Sulfated galactans, SG supplemented diet, immune-related genes, antibacterial activity, Vibrio parahaemolyticus.

References:

[1] Wongprasert K, Rudtanatip T, Praiboon J. Immunostimmulatory activity of sulfated galactans isolated from the red seaweed Gracilaria fisheri and development of resistance against white spot syndrome virus (WSSV) in shrimp. Fish Shellfish Immunol 2014; 36: 52 – 60

[2] Rudtanatip T, Withyachumnarnkul B, Wongprasert W. Sulfated galactans from Gracilaria fisheri bind to shrimp haemocyte membrane proteins and stimulate the expression of immune genes. Fish Shellfish Immunol 2015; 47: 231 – 238


No conflict of interest has been declared by the author(s).