Pneumologie 2019; 73(S 01)
DOI: 10.1055/s-0039-1678077
Posterbegehung (P07) – Sektion Pneumologische Onkologie
SCLC, Mesotheliom, Grundlagen
Georg Thieme Verlag KG Stuttgart · New York

Non-small cell lung cancer cells induce the expression of adipokines in brown adipose tissue in the context of cancer cachexia

A Frille
1   Abteilung für Pneumologie, Universitätsklinikum Leipzig, Integriertes Forschungs- und Behandlungszentrum (Ifb) Adipositaserkrankungen, Universitätsmedizin Leipzig
,
H Kuhn
2   Universitätsklinikum Leipzig, Dept. für Innere Medizin, Abt. Pneumologie
,
T Ebert
3   University of Leipzig, Dep. of Endocrinology & Nephrology, IFB Adipositaserkrankungen
,
HJ Seyfarth
2   Universitätsklinikum Leipzig, Dept. für Innere Medizin, Abt. Pneumologie
,
H Wirtz
2   Universitätsklinikum Leipzig, Dept. für Innere Medizin, Abt. Pneumologie
› Author Affiliations
Further Information

Publication History

Publication Date:
19 February 2019 (online)

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    Introduction Brown adipose tissue (BAT) increases energy expenditure and might be involved in the development of cancer cachexia. Non-small cell lung cancer (NSCLC) cells might induce BAT activation via an adipokine signaling that promotes cancer cachexia.

    We aimed to find out whether NSCLC cells influence the expression of adipokines in both undifferentiated preadipocytes and differentiated BAT that are involved in the differentiation of BAT.

    Methods Preadipocytes and BAT were cultured for six days with conditioned media derived from six NSCLC cell lines (A549, H322, PC9, H1650, H1299, H460), respectively. Media was changed every three days. Protein levels of the five key-adipokines adiponectin, fibroblast growth factor 21 (FGF21), irisin betatrophin, and neuregulin 4 (NRG4) were investigated in both supernatants and cell lysates of preadipocytes and BAT by enzyme-linked immunosorbent assay.

    Results Undifferentiated preadipocytes and differentiated BAT released higher FGF21 protein levels into the supernatants when cultured with conditioned medium derived from PC9 cells as compared to control medium. Higher irisin levels were observed in the supernatants of both preadipocytes and BAT when cultured with conditioned media derived from H322 cells. Lung cancer cell-derived media induced a decreased release of betatrophin protein level into preadipocytesʼ supernatants. Adiponectin and NRG4 protein expressions were only found in cell lysates: Adiponectin remained undetectable preadipocytes, while it was upregulated in BAT when cultured with conditioned media, regardless of NSCLC cell line. NRG4 protein expression was remarkably increased in undifferentiated and differentiated BAT when cultured with H322 conditioned medium.

    Conclusions PC-9 and H322 cells media may potentially induce preadipocyte differentiation towards differentiated BAT through specific FGF21 and irisin signaling, respectively. Therefore, NSCLC cells might play a role in the differentiation of BAT, which in turn might be involved in the development of cancer cachexia.


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