CC BY-NC-ND 4.0 · Laryngorhinootologie 2019; 98(S 02): S200
DOI: 10.1055/s-0039-1686887
Abstracts
Tissue Engineering/Stem Cells

Effects of a combined static and dynamic cultivation on the matrix synthesis of chondrocytes

E Goldberg-Bockhorn
1   Universitäts-HNO-Klinik, Ulm
,
R Riepl
2   HNO Uniklinik Ulm, Ulm
,
U Wenzel
3   Institut für Medizintechnik und Mechatronik, Hochschule Ulm, Ulm
,
F Sommer
2   HNO Uniklinik Ulm, Ulm
,
M Wigand
2   HNO Uniklinik Ulm, Ulm
,
J Döscher
2   HNO Uniklinik Ulm, Ulm
,
L Körber
4   Lehrstuhl für Bioverfahrenstechnik Universität Erlangen-Nürneberg, Erlangen
,
M Heßling
3   Institut für Medizintechnik und Mechatronik, Hochschule Ulm, Ulm
,
TK Hoffmann
2   HNO Uniklinik Ulm, Ulm
› Author Affiliations
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    Background:

    Newly synthesized cartilage from decellularized porcine nasal septal cartilage (DECM) produced by tissue engineering could serve as an alternative material for the reconstruction of congenital and acquired cartilaginous defects in the future. Former studies showed that the migration of human nasal septal chondrocytes (hnCh) into the DECM can be accelerated in an automated bioreactor (BR), whereas the static culture provides a better cell differentiation and new synthesis of matrix (nMS). The current study investigates the effects of a combination of both culture techniques on the cell migration and the nMS.

    Methods:

    DECM were seeded with hnCh and cultivated in the BR. The constructs were transferred to the static culture after 14 and 28 days, followed by further cultivation for 14 or 28 days respectively. The sole static culture (sC) and sole dynamic culture (dC) served as control groups. The increase in cell number and the migration of the hnCh were examined histologically and with quantifluor assay. Immunohistochemistry, DMMB assay and rtPCR detected and quantified the nMS.

    Results:

    Initially, hnCh migrated more efficiently into the matrix in the BR compared to the sC. The transfer of the constructs from the dC to the sC resulted in an increase of nMS and a better differentiation compared to the sC alone. This effect was exceptionally obvious after 14 days inside the BR followed by sC.

    Conclusion:

    The positive effects of the single cultures of seeded DECM can be fostered by the combination of the dC and sC. Further studies should examine in detail which parameters cause the different behavior of the hnCh in both single culture techniques.


    #
    Dr. med. Eva Goldberg-Bockhorn
    Universitäts-HNO-Klinik,
    Frauensteige 12, 89075
    Ulm

    Publication History

    Publication Date:
    23 April 2019 (online)

    © 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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