In vivo-testing of porcine decellularized extracellular cartilage matrix for septal reconstruction in a rabbit model

Introduction Septal cartilage defects after surgery or trauma are reconstructed using autologous cartilage. Harvest and preparation of cartilage are time-consuming and lead to donor site defects, therefore alternatives are needed: In this study we examine the reconstruction of cartilage defects using a decellularized porcine cartilage matrix (DECM). The matrix is loaded with growth factors (PDGF-BB) and implanted instead of the nasal septum. The aim of this study is to examine if DECM induces local progenitor cells and if the defect is filled with vital cartilage tissue.

Material/Methods In-vivo experiments were conducted with New Zealand White Rabbits (14-16 weeks, n=4 per group). The septum was removed under general anesthaesia and a DECM scaffold of the same size (group 1 without growth factor, group 2 with PDGF-BB) or the original septum (control) was reimplanted. 4 weeks later an MRI (evaluation of septal deviation and shrinkage) and histological examinations of septum and surrounding tissue were performed.

Results The MRI showed an acceptable form stability of the implanted scaffolds. No relevant shrinkage or increased septal defects were seen. Irrespective of the reimplanted septum (autologous or DECM) some rabbits showed septal deviations. Obstructed nasal breathing was not observed. The early histological examinations to assess the biocompatibility show no massive inflammatory reaction.

Conclusion Reconstruction of cartilage defects using a DECM in a rabbit model is possible, the biocompatibility needs to be further examined.

Poster-PDF A-1432.pdf

DFG-Projekt: Ro 2207/5-1

Conflict of interest

Der Erstautor gibt keinen Interessenskonflikt an.

Publication History

Article published online:
13 May 2021