Geburtshilfe Frauenheilkd 2021; 81(06): e10
DOI: 10.1055/s-0041-1730470
Endokrinologie & Reproduktionsmedizin

The fate of SUSD2+ endometrial mesenchymal stem cells during decidualization

T Gorsek Sparovec
1   Department of Obstetrics and Gynaecology, Medical University of Graz, Austria, URL: tina.gorsek@medunigraz.at
,
U Markert
2   University Hospital Jena, Department of Obstetrics, Germany
,
P Reif
1   Department of Obstetrics and Gynaecology, Medical University of Graz, Austria, URL: tina.gorsek@medunigraz.at
,
W Schoell
1   Department of Obstetrics and Gynaecology, Medical University of Graz, Austria, URL: tina.gorsek@medunigraz.at
,
C Gargett
3   Monash University, Department of Obstetrics and Gynaecology, Australia
,
D Gold
1   Department of Obstetrics and Gynaecology, Medical University of Graz, Austria, URL: tina.gorsek@medunigraz.at
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Einleitung Regeneration of endometrium in premenopausal women is maintained by endometrial mesenchymal stem cells (eMSC) upon exposure to estrogen and progesterone. eMSC located in the perivascular niche of endometrium express so called Sushi Domaine Containing 2 (SUSD2) [1]. The role of these eMSCs in decidualization and implantation is unknown. Our aim was to determine the effect of progesterone on the stemness of SUSD2+ eMSC isolated from non-pregnant uterine samples. A secondary objective was to characterize the functional capacity of SUSD2+ eMSC isolated from uterus at term pregnancy and compare it with the capacity of those isolated from non-pregnant uterine samples.

Material und Methodik First, SUSD2+ eMSCs from non-pregnant uterine samples were treated with progesterone to induce decidualization. Changes in membrane markers and gene expression profile of various genes involved in the decidualization were determined. In addition, the abundance of SUD2+ eMSC was determined by immunohistochemistry in the non-pregnant and both 1st trimester and full-term uterine samples. To characterize functional capacity, SUSD2+ cells were differentiated and a CFU assay was performed.

Ergebnisse Decidualization induced changes in the gene expression profile of several genes involved in the decidualization process suggesting the importance of SUSD2+ eMSC in the embryo implantation. Second, results show that SUSD2+ eMSC persist located in the perivascular niche throughout pregnancy. Histological analysis revealed statistically significant lower abundance of eMSC in 1st trimester and term samples compared to non-pregnant samples (p= 0,0296 and 0,005, respectively). The ability to differentiate into mesenchymal lineages and form colonies was not significantly different between the cells isolated from non-pregnant and pregnant uterine samples.

Zusammenfassung Our results suggest that eMSCs play an important role in the course of endometrial decidualization and later in embryo implantation.

Interessenskonflikt I hereby declare that there is no conflict of interest


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  • Literatur

  • 1 Masuda H. et al., A novel marker of human endometrial mesenchymal stem-like cells. Cell Transpalnt, 20212. 21 (10) :p. 2201-14
    CrossrefPubMedGoogle Scholar

Publication History

Article published online:
02 June 2021

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  • Literatur

  • 1 Masuda H. et al., A novel marker of human endometrial mesenchymal stem-like cells. Cell Transpalnt, 20212. 21 (10) :p. 2201-14
    CrossrefPubMedGoogle Scholar