Buchholzia coriacea Leaves Attenuated Dyslipidemia and Oxidative Stress in Hyperlipidemic Rats and Its Potential Targets In Silico

Abstract The study aimed to investigate how the solvent extract of Buchholzia coriacea (BCE), a widely known hypolipidemic agent, could contribute to hyperlipidemia treatment and identify the potential bioactive compounds. We studied Wistar albino rats, dividing them into seven groups: the normal control, normal rats treated with 400 mg/kg.b.wt of BCE (NRG group), the hyperlipidemic control (HPC group), hyperlipidemic rats treated with atorvastatin, a standard control drug (SC group), as well as 200, 400, and 800 mg/kg.b.wt of BCE extract respectively (T1, T2, T3 groups). The potential compounds that functioned in BCE extract were analyzed by in silico binding to acetyl-CoA carboxylase (ACC) and fatty acid synthase (FASN). The binding affinities and drug-like properties of the compounds were determined using virtual screening and absorption distribution metabolism excretion and toxicity prediction analysis. The gas chromatography-mass spectrometry analysis identified alkaloids, saponins, flavonoids, phenols, terpenoids, and 44 chemical compounds in the leaf extract of BCE. BCE significantly reduced the levels of triacylglycerol, total cholesterol, low-density lipoprotein, very low-density lipoprotein, atherogenic coefficient, atherogenic index, and coronary risk index, while enhancing the levels of high-density lipoprotein and cardioprotective index in comparison to the HPC group. The BCE reduced malondialdehyde quantities, which exhibit high levels in HPC. Superoxide dismutase and glutathione peroxidase activities as well as glutathione levels, which are otherwise reduced in HPC, were increased upon the BCE treatment. Among the identified BCE compounds, lupenone and 2,7-dimethylnaphthalene exhibited the highest binding affinities to ACC and FASN, suggesting that these two compounds might be the bioactive BCE components displaying hypolipidemic properties. BCE is found to be beneficial in blocking hyperlipidemia through the modulation of lipid profile, the protection of cardiovascular function, as well as the suppression of oxidative stress. BCE may be a natural source for exploring novel drugs for the treatment of dyslipidemia.


Introduction
There is an increasing reliance on medicinal plants in the case of various disease treatments both in Africa and worldwide, 1 due to ingested chemical deposits that might exhibit therapeutic benefits.Managing the cholesterol, especially low-density lipoprotein cholesterol (LDL-c), levels in the body is reportedly crucial to prevent cardiovascular disease-associated conditions. 2,3cetyl-coenzyme A (acetyl-CoA) is a rate-limiting enzyme in fatty acid metabolism. 4Malonyl-CoA, the product of acetyl-CoA carboxylase (ACC) catalysis, is essential for triggering fatty acid synthase spiral reactions, another essential enzyme for lipid biosynthesis. 5Abnormal fatty acid metabolism due to increased fatty acid synthesis by the fatty acid synthase activities initiated by the ACC activities is a hallmark of glycolipid metabolic diseases. 6Therefore, the inhibitors of these enzymes were the focus of the preceding search for therapies against various human diseases such as diabetes mellitus, cancer, obesity, and metabolic syndrome, aiming at the prevention of hyperlipidemia (i.e., increased blood lipid levels).As cells use glucose, pyruvate is the end product of the first process of glucose metabolism, known as the glycolytic pathway.Pyruvate undergoes decarboxylation in the mitochondria by an intermediate acetyl-CoA, then it becomes the substrate for the next step in the energy cycle, the tricarboxylic acid cycle, supplying a certain amount of adenosine triphosphate (ATP). 5The product ACC, along with malonyl-CoA, serves as a substrate for fatty acid synthase (FASN), an enzyme responsible for the biosynthetic process catalysis of the FASN spiral, in a nicotinamide-adenine-dinucleotide-phosphatereduced (NADPH)-dependent reaction.
Years of research effort in natural products, chemistry, and biochemistry resulted in advances in the design and discovery of numerous inhibitors of these enzymes.Buchholzia coriacea (BCE) is a forest tree of the Capparaceae family. 7An earlier study revealed that the anti-inflammatory, antiulcer, antihelminths, anti-inflammatory, antioxidant, antihyperlipidemic, antihypercholesterolemic, antiatherogenic, antitrypanosomal, antimodulatory, antispasmodic, antidiarrhea, and antifertility BCE plant properties have been scientifically validated. 7These BCE properties endowed this plant with significant advantages as a medicinal plant compared with other plants of the same class.Therefore, research into BCE and its derivatives as a natural plant source is necessary, as it could represent a further alternative in the search for hypolipidemic agents in the treatment of dyslipidemia.
Moreover, the seeds of the plant are edible with a peppery taste and are known for their high medicinal value, giving the plant its common name, wonderful kola. 7This plant has been used to treat various diseases in different parts of Africa over the years.Recent studies of this plant's seed extract demonstrated that it is a good source of energy and possesses antibacterial, antidiabetic, hypolipidemic, antioxidant, and antiulcer activities. 8This study thus aimed at investigating the hypolipidemic potential of BCE both in vivo and in silico against ACC and FASN, key proteins that are involved in the lipogenesis pathway, potentially providing opportunities to search for therapies against hyperlipidemia and associated disease complications.

Plant Collection
BCE plants were obtained from a farm in Abakaliki, Ebonyi State, Nigeria, and taken to Evangel University Biochemistry Laboratory for authentication by Dr. Bruno Ado at the Biology Division, Department of Biotechnology, then dried at room temperature between 23°C and 25°C.

Crude Extract Preparation
The leaves were cut into pieces and dried at room temperature (25-29°C).The dried leaves were ground and soaked in methanol at a sample-to-solvent ratio of 1:3.The suspension was stirred in an electric mixer and left to stand for approximately 48 hours at room temperature, then filtered. 9The filtrate was then concentrated as described previously. 10

Phytochemical Plant Extract Screening
2][13] Briefly, when testing for alkaloids, 0.1 g of the extract was supplemented with 10 mL of acidified alcohol, the mixture was then heated and filtered to investigate the potential alkaloid content.Next, 1 mL of filtrate was supplemented with 0.4 mL of diluted ammonia and 1 mL of malondialdehyde quantities, which exhibit high levels in HPC.Superoxide dismutase and glutathione peroxidase activities as well as glutathione levels, which are otherwise reduced in HPC, were increased upon the BCE treatment.Among the identified BCE compounds, lupenone and 2,7-dimethylnaphthalene exhibited the highest binding affinities to ACC and FASN, suggesting that these two compounds might be the bioactive BCE components displaying hypolipidemic properties.BCE is found to be beneficial in blocking hyperlipidemia through the modulation of lipid profile, the protection of cardiovascular function, as well as the suppression of oxidative stress.BCE may be a natural source for exploring novel drugs for the treatment of dyslipidemia.
chloroform, then the solution was gently shaken.The chloroform layer was extracted using 2 mL of acetic acid, then separated into two sections, with one being supplemented with Mayer's and the other with Dragendorff's reagent.For the alkaloid test, the appearance of a cream (when using Mayer's reagent) or a reddish-brown precipitate (when using Dragendorff's reagent) was considered positive.When testing for saponins, the production of an emulsion after adding three drops of olive oil to the froth created by mixing 0.1 g of extract with 1 mL of distilled water indicated saponin presence.To detect flavonoids, we performed an alkaline reagent test.The presence of flavonoids was indicated by the test solution developing a strong yellow color following its supplementation with a few drops of sodium hydroxide solution, which turns colorless upon the addition of a few drops of diluted acid.Using Salkowski's test, terpenoids were identified when strong sulfuric acid and 0.4 mL of chloroform were added to 0.1 g of extract, and a reddish-brown coloring formed on the interface, indicating terpenoid presence.When 50 mg of the extract was dissolved in 5 mL of distilled water and a few drops of a neutral 5% ferric chloride solution were added to it, the development of a dark green hue was considered a sign of phenolic compound presence.[13] Gas Chromatography-Mass Spectrometry Analysis of the Extracts Batch processing of the solvent-extracted sample was used in the process of sample preparation of gas chromatographymass spectrometry (GC-MS).Therefore, the chemicals were efficiently extracted from the sample within less time.The components of the sample were separated using the stationary phases of 5% diphenyl-95% dimethyl polysiloxane before loading.The chemical composition of the extract was then analyzed using the Agilent GC-MS (Model No. 19091S-933) equipped with an HP-1 millisecond capillary column (0.25 mm id.Â 30 m specification length Â 0.25 mL film thickness) and an autosampler as described previously. 14riefly, the conditions were maintained as follows: initial temperature and time, 70°C for 2 minutes, then raised to 350°C at a rate of 20°C/min and maintained for 20 minutes; 350°C for MSD transfer line heater; the carrier gas was helium at a flow rate, split ratio, and run time of 1.2 mL/min, 10:1, and 32.50 minutes, respectively.

Animals
We used 42 healthy adult male albino rats in this study, purchased from the farm of Daniel Okoro in Abakaliki, Ebonyi State, Nigeria.The animals were given a week to get used to the laboratory environment before the start of the experiment.The animals were kept in cages and given a regular pellet as diet and water ad libitum.

Experimental Design
A total of 42 male albino rats, weighing between 150 and 180 g, were divided into seven experimental groups (n ¼ 6).Hyperlipidemia induction in rats was established by three intraperitoneal administrations of poloxamer P-407 (BASF Corporation; Mount Olive, New Jersey, United States) as follows: initial induction at 1g/kg.b.wt and two additional doses (one at 0.5 g/kg of P-407 on day 7 and another on day 11 following the initial induction) for maintaining hyperlipidemia. 15Hyperlipidemia was confirmed 48 hours after the first hyperlipidemic induction.Blood was collected via cardiac puncture and used to ascertain the hyperlipidemic state of the rats using a Cardio Check device (Mission Cholesterol Meter, model number: CCM-111, Germany).Atorvastatin (Ceno Pharmacy Ltd., Ebonyi State, Nigeria) was used as the reference drug.Normal rats were treated with 0.5 mL normal saline (the normal control [NC] group) and 400 mg/kg.b.wt BCE (the normal rats treated [NRT] group).Hyperlipidemic rats were treated with 0.5 mL normal saline (the HPC group), 10 mg/kg.b.wt.atorvastatin (the standard control [SC] group), as well as 200, 400, and 800 mg/kg.b.wt of BCE (hyperlipidemic treated groups 1, 2, and 3 [T1, T2, and T3]), administered orally as earlier used daily for 21 days. 16The treatment started 48 hours after the initial induction and immediately upon the confirmation of the hyperlipidemic state, while lower doses of P-407 (0.5 g/kg.b. wt) for hyperlipidemia maintenance were repeated on days 7 and 11 following the initial induction) as earlier used. 15The animals in all groups were allowed free access to feed and water and the treatment lasted for 21 days, then the animals were sacrificed under chloroform inhalation anesthesia and the samples were obtained.Biochemical testing was then performed on the recovered supernatant (serum).This study was approved both by the Institutional Ethical Committee on Animal Rights and the Department of Biochemistry Ethical Committee on Research and Innovation (FUHSO/ET/BCH/ 22/002).

Serum Lipid Determination
Spectrophotometric techniques were used to measure the serum level of total cholesterol (TC), as explained by a reported study. 17The level of serum triacylglycerols (TAG) was assessed using the Randox kit (Randox Laboratories Limited, United Kingdom). 18Serum level of high-density lipoprotein cholesterol (HDL-C) was measured by the Warnick and colleague's method. 19The following equation was used to determine the serum level of LDL-C following Friedewald et al's protocol 20 : TG/5-HDL-c ¼ LDL-c (mg/dL).The value was given in milligrams per deciliter.The formulas for calculating the atherogenic coefficient (AC), atherogenic index (AI), and coronary risk index (CRI) were as previously described: AC ¼ [TC -HDL-C/HDL-C], AI ¼ Log[TG/HDL-C], and CRI ¼ [TC/HDL-C], respectively.The assessment of the cardioprotective index (CPI) was based on the HDL-C/LDL-C ratio. 21

Oxidative Stress Indicator Evaluation
Thiobarbituric acid reactive substances (TBARS) were measured as lipid peroxidation markers using the Buege and Aust's technique. 22The following antioxidant activity tests were performed.Superoxide dismutase (SOD) was measured using a previously described technique for SOD activity determination. 23The reduced glutathione test (GSH) procedure was based on the concept first proposed by Ellman 24 in 1959 and revised by Hu in 1994. 25The technique we applied to assess glutathione peroxidase (GPX) activity was the one Hu and Ellman adapted and described in the assessment of sulfhydryl groups. 24 Silico Studies

Target Preparation
The ACC (PDB-ID: 1UYS) and FASN (PDB-ID: 2PX6) structures were extracted from the Protein Data Bank (PDB) structure database (https://www.rcsb.org/).An open-source molecular editor was used to import the structures (Discovery Studio Visualizer 4.0).The structures were saved in PDB format after co-crystal ligand and heteroatom removal.Chimera UCSF was used to optimize the structure, consisting of 1,000 steps of the steepest descent followed by 1,000 steps of the conjugate gradient of the energy minimization method.The ligands were accessed from the PubChem database (https://pubchem.ncbi.nlm.nih.gov/), then their structures were loaded into the DS-Visualizer and saved as PDB files.We performed the virtual screening of 46 compounds based on the binding energy scores with targeted macromolecules ACC (1UYS) and FASN (2PX6) using the PyRx software. 26mong the 46 ligands, we discovered that lupenone (92158) and 2,7-dimethylnaphthalene (11396) displayed the maximum dock scores.ACC (1UYS)-lupenone and FASN (2PX6)-2,7-dimethylnaphthalene displayed binding affinity ratings of À8.8 and À7.7, respectively.Based on the binding affinities, two ligands were selected for verification using the AutoDock MGL tool 1.5.6.From each ligand, the nine best-docked poses with the highest to lowest binding energies were obtained, out of which the best-docked pose with the highest binding energies was considered for redocking and further analysis.

Molecular Docking Studies
The receptor protein for docking was produced in the Auto-Dock format. 27To construct the receptor lattice, we used residues around 1 of 1UYS and 2PX6 associated with the cocrystal of lupenone and 2,7-dimethylnaphthalene. Receptors and ligands were saved in a pdbqt format for later use with the MGL program.Vina was started from a command prompt on the command line.In the setup, the standard grid spacing was 0.425 and the completeness was set to 8. The output files were in a pdbqt format and were submitted for analysis with PyMol and the Discovery Studio Visualizer 2021.The 1UYS and 2PX6 structures were reduced using the steepest descent method (1,000 steps) followed by the inclusion of the AMBER ff4 force field before the docking research was performed with the relevant ligands.Before the interaction studies, the protonation states of the 1UYS and 2PX6 involved were tested for neutralization.Autodocking v 4.2.6 was used to perform the molecular docking experiments.Both the receptor and the ligands were created by combining polar hydrogen bonds with Kollman and Gasteiger charges.Finally, both receptor and ligand molecules were saved in a pdbqt format after the nonpolar hydrogen atoms were combined.A mesh box was produced with the values as follows: X ¼ 20, Y ¼ 18, and Z ¼ 32 with a distance of 0.3.The protein-ligand complex docking experiments were performed with the Lamarckian Genetic Algorithm to obtain the lowest free energy of binding (G).

Lupenone Lead Optimization
The most pronounced concern for the pharmaceutical industry in new drug molecule detection and development is the expected bioavailability, which is influenced by solubility. 28In this study, though lupenone exhibited strong ACC binding and inhibition potentials, representing a strong new drug development candidate for dyslipidemia management, the carbon skeleton of this compound presented a major concern in the future drug development process due to its low solubility features and complex carbon structure.Hence, lupenone (►Fig.1A) was optimized to a better lupenone derivative (►Fig.1B using the ADMETopt web server. 29►Fig.1C, D presents the optimized molecules (I, II, and III) and 2,7-dimethylnaphthalene, respectively.In this case "III" was used for further docking in the AutoDock MGL tool 1.5.6 with the ACC (1UYS).The lupenone derivative was drawn using the software Marvin Sketch from the Chemaxon Buchholzia coriacea and Dyslipidemia Uti et al. e144 web server (https://chemaxon.com/products/marvin) and saved in a PDB format before docking preparation.

Absorption Distribution Metabolism Excretion and Toxicity/Pharmacokinetics Prediction Analysis of Top-Posed Compounds
The absorption distribution metabolism excretion and toxicity (ADMET)/pharmacokinetics prediction analysis of the top-posed compounds was performed using the online web servers SwissADME (http://www.swissadme.ch/) 30nd admetSAR (http://lmmd.ecust.edu.cn).This Web site allows for calculating physicochemical descriptors and predicting ADME parameters, pharmacokinetic properties, drug-like nature, and medicinal chemistry friendliness of one or more small molecules in the drug discovery process using the canonical Simplified Molecular Input Line Entry Specification of the investigated compounds or potential drugs.

Statistical Analysis
The differences between the treated and control groups were statistically evaluated using a one-way analysis of variance coupled with a one-sample t-test.The results were expressed as the mean AE standard deviation.GraphPad Prism7 was used to complete all statistical calculations.p-Values of <0.05 were considered statistically significant.

Qualitative and Quantitative Phytochemical BCE Composition
The qualitative BCE leaf extract analysis indicated the presence of tannins, flavonoids, terpenoids, alkaloids, saponins, and phenols, while steroids, cardiac glycosides, and reducing sugars were not in a detectable amount (►Table 1).►Fig. 2 depicts the quantitative analysis of phytochemical constituents, indicating a high-level presence of flavonoids with a significant presence of saponins, alkaloids, terpenoids, phenols, and tannins.

GC-MS-Detected Methanolic BCE Extract Chemical Profiles
The chemical composition of the extract was analyzed using Agilent GC-MS (►Table 2).We detected 44 chemical compounds from the plant using the GC-MS technique, which we summarized here indicating the PubChem ID, molecular formula, and chemical quality.
Extract Treatment Effects on Lipid Profiles in P-407-Induced Hyperlipidemic Rats ►Fig. 3 presents treatment effects on P407-induced hyperlipidemic rats.Our data showed that the TAG level was significantly increased in the HPC group (p < 0.05) compared with the NC; however, the treatment groups (SC, NRT, and T1-T3) significantly reduced TAG leaves to those similar to NC.Similar observations were seen in TC, LDL, and very lowdensity lipoprotein (VLDL) in different groups.However, a reciprocal effect could be observed on serum HDL concentration, where reduced HDL in HPC compared with NC was increased upon the various treatments (SC, NRT, and T1-T3).

Treatment Effect on Cardiovascular Function Indices
Next, we assessed the effect of leaf extracts on expression levels of cardiovascular functional markers (AC, AI, CRI, and CPI).As shown in ►Fig.4, in comparison to atorvastatin, the gold standard medication, a high dose of BCE was more effective at defending the rats against cardiovascular risk markers.AC, AI, and CRI were significantly enhanced in the HPC group, and the changing trend was significantly reversed by additional treatment (SC, NRT, T1-T3).CPI levels, significantly reduced in the HPC group, was markedly increased upon different treatment (SC, NRT, T1-T3) (p < 0.05).

Treatment Effect on Lipid Peroxidation and Oxidative Stress Markers
The lipid peroxidation marker TBARS-malondialdehyde (MDA) levels in the HPC group significantly (p < 0.05) increased, and with additional treatment of leaf-extracts Note: þ: present; À: not detected.

Buchholzia coriacea and Dyslipidemia Uti et al. e146
(T1-T3) and the standard drug (SC), the changing trend was significantly reversed (p < 0.05) (►Fig.5).Similarly, SOD and the activities of GPX and GSH were significantly decreased in HPC rats relative to NC rats (►Fig. 5,p < 0.05).However, these SOD-and glutathione-related characteristics significantly and dose-dependently (p < 0.05) increased following the leaf extract and standard drug treatments.

Virtual Compound Screening
The ACC-lupenone and FASN-2,7-dimethylnaphthalene complexes (1UYS-92158 and 2PX6-11396, respectively) exhibited binding affinity scores of À8.8 and À7.7 binding affinity dock scores, which were better than those of the two standard inhibitors of these proteins (UYS -71528744 [firsocostat] with À6.7 and 2PX6-9881506 [C75 trans] with À5.7).These best two molecules were further re-docked at the 1UYS and 2PX6 binding cavities.Out of 46 ligands screened for both receptors, two ligands lupenone and 2,7-dimethyl naphthalene (Chem ID: 92158 and 11396, respectively in ►Table S1 [Supporting Information]) with the Rmsd score 2.507 with 1UYS-92158 and Rmsd score 0.329 with 2PX6-11396 showed the lowest binding energies, suggesting the highest binding affinities.This binding energy-based virtual screening gave us a vivid idea of the best ligand with the highest affinity to ACC (1UYS) and FASN (2PX6).The 3D representation of these interactions are as shown in ►Fig. 6.

Molecular Docking Studies
Molecular interaction in re-docking studies of the lupenone ligand with ACC (1UYS) highlighted a well-defined binding pocket constituted of leucine, proline, alanine, asparagine, tyrosine, leucine, and glutamine residues, where the ligand bound to the core of the pocket with a binding energy of (D) À12 kcal/mol and inhibitory concentration (K i ) of 1.59 μmol/L.Moreover, after re-docking with the 2,7-dimethylnaphthalene ligand with FASN (2PX6), it displayed a well-defined binding  pocket constituted of valine, leucine, glutamine, phenylalanine, glycine, isoleucine, and serine residues, where the ligand bound to the core of the pocket with a binding energy of (D) À6.99 kcal/mol and K i of 11.21 μmol/L.This is indicated in the Supporting Information (►Fig.S1 [available in the online version]).From ►Fig.S1 (available in the online version), the significant residues of Pro1817, Val1818, Pro1811, and Leu2130 are attached to the ligand via van der Walls inter-actions, while residues Lys2137, Glu2133, Tyr2134, Asn2131, Tyr2179, Glu2176, Arg2127, Ile1820, Ile1887, Gln1983, Gln1944, Mse1816, and Asn1815 are bound by alkyl interactions.From ►Fig.S1B (available in the online version), the significant residues of Ile2250, Lys2426, Ser2422, and Gln2374 are attached to the ligand through van der Walls interactions, while residues Phe2375, Ala2419, and Leu2222 are bound by alkyl interactions, and Phe2371 and Phe2423 are bound via  Pi-Pi interactions.The post-docking analysis revealed that the optimized lupenone derivative exhibited a higher binding affinity of À8.9 compared with parent lupenone with a binding energy of À8.8.Moreover, the two-dimensional binding interaction of the optimized derivative, as revealed by discovery studio visualizer 2020, unraveled the presence of hydrogen bond interactions with ACC (►Fig.S1C [available in the online version]), a more stable bond absent in the parent lupenone molecule.

ADMET/Pharmacokinetics Predictions of Top-Posed Compounds
The analysis of the lead compounds lupenone (Chem ID: 92158) with its optimized derivate and 2,7-dimethylnaphthalene (Chem ID: 11396) using Lipinski's rule of five, Ghose, and Veber rule is presented in the Supporting Information (►Table S2 [available in the online version]) for its druglikeness, while the absorption and metabolism profiles of these compounds are presented in the ►Tables S3 and S4 (Supporting Information) with the toxicity profile in ►Table S5 (Supporting Information).We observed different degrees of violation of the three rules for drug-like properties as indicated.It was only the optimized lupenone derivative that showed high human gastrointestinal tract absorption, while lupenone and 2,7-dimethylnaphthalene exhibited low GIT absorption profiles.These ligands also exhibited varying degrees of cytochrome-P variant inhibition profiles with different toxicological properties toward certain target organs and features.

Discussion
In this study, we investigated the potential effect of BCE leaf extracts on hyperlipidemia and oxidative stress indicators in Wistar rats.In addition, we examined the BCE leaf extract as a potential source of ACC and FASN inhibitors and potential hypolipidemic agents in silico.
Active ingredients are reportedly implicated in the negative correlation between plant intake and the risk of oxidative stress-related illnesses, including cardiovascular diseases. 31is study has identified earlier a significant presence of phytochemicals with an abundance of flavonoids.We detected approximately 44 active ingredients in the plant extract.The significant presence of flavonoids indicates that BCE would be a good source of antioxidants.According to a theory, flavonoids downregulate the expression of NADPH oxidase isoforms, thereby preventing NADPH oxidase from producing superoxide and hence averting oxidative stress. 32Saponins were also present in the extract in significant amounts, which are highly implicated in lipid metabolism modulation.A proposed mechanism would be that saponins reduce lipid accumulation via autophagy induction. 33his study also focused on how BCE affects lipid profiles in HPC rats.P-407 significantly increased TAG, TC, LDL, and VLDL while reducing HDL levels, suggesting sustained hyperlipidemia in the tested animals, which was consistent with previous findings. 34However, the P-407-induced changing trend in the lipid profiles was significantly reversed upon the BCE leaf extract treatment.This may be due to the presence of saponins in the plant extracts.Saponins reportedly up-regulate acyl-CoA dehydrogenase and carnitine palmitoyltransferase 1, which are involved in oxidation and FASN an ACC downregulation. 35We observed similar modulatory effects on cardiovascular function indices with positively modulated values such as increased CPI and reduced AC, AI, and CRI.
We also investigated the in vivo antioxidant activities of the BCE extract.MDA is one of the consequences of polyunsaturated fatty acid peroxidation in the cells and one of the main markers of lipid peroxidation in oxidative stress, inflammation, and various medical problems; it is thus an important oxidation product.We assessed oxidative stress biomarker levels and observed that the level of TBARS-MDA, an oxidative stress indicator, was significantly higher in the HPC group than in the NC group.However, its reduction was observed in BCE-treated groups, which is assumably caused by the potential of the extract to neutralize hydroxyl and peroxyl radicals through antioxidant activities, probably due to the significant presence of flavonoids in the extract.Moreover, the activities of SOD, GPX, and the concentration of GSH decreased in HPC and similarly increased upon extract administration.Several factors could be responsible for this but the most relevant is potentially the abundant presence of flavonoids in the plant extracts.The antioxidative effect of flavonoids has been documented earlier. 32CC and FASN are two major regulators in fatty acids biosynthesis and fatty acids play several important roles in the body, including their impact as building blocks for phospholipids and glycolipids, target proteins for membranes, an energy-rich fuel source, and fatty acid derivatives, and are used as hormones and intracellular messenger substances. 22Excessive fat storage could lead to serious health consequences; a condition simply known as hyperlipidemia. 23Hyperlipidemia cannot be considered a disease in its own right.However, this underlying pathology often leads to serious and ultimately even lethal illnesses.Fatty acids are usually synthesized from acetyl-CoA, a process that requires ATP, biotin, Mg 2þ , and Mn 2þ . 37The ACC used in this study is the rate-limiting enzyme in fatty acid biosynthesis, while the remaining reactions in fatty acid biosynthesis are executed by a multifunctional protein, fatty acid synthetase (FAS). 38The ACC (1UYS) and FASN (2PX6) interaction with lupenone and 2,7-dimethyl naphthalene, compounds featuring higher binding poses derived from BCE, have been previously reported to possess hypolipidemic potentials, as documented in earlier studies, 7 which is a validation of the present study.
This study aimed at identifying the best molecule screened from virtual screening of interacting 44 derived chemical compounds via GC-MS (►Table 2) with ACC (1UYS) and FASN (2PX6) and comparing their binding potentials to the standard inhibitors of these proteins' essential in fatty acid synthesis.The results were of particular interest as the two compounds lupenone and 2,7-dimethylnaphthalene (►Fig.1A, D) exhibited higher binding affinities compared with standard inhibitors.Lupenone is a triterpenoid 39 and it has a role as a metabolite, 39 deriving from a hydride of lupane while 2,7-dimethylnaphthalene is a dimethylnaphthalene. 40 Triterpenoids, the class of compounds to which lupenone belongs, reportedly exhibit diverse biological activities due to their structural diversity, such as anticarcinogenic, hepatoprotective, antidiabetic, anti-inflammatory, hypolipidemic, antioxidant, and antimicrobial effects, 41 which is in good agreement with the observations of this study.
The detailed drug-like properties of the lead compounds, as revealed by the ADMET analysis, are shown in the Supporting Information (►Table S2).Some of the results include strong compliance with Lipinski, Ghose, and Vebers rules, with low inhibition of some important drug-metabolizing enzymes (CYP1A2 CYP 2C19, CYP 2D6, CYP 3A4, and CYP 2C9) and low potential organ toxicity (►Table S3, S4 [Supporting Information]).The ADMET properties of these compounds indicated that they inhibit certain CYP enzymes and might interfere with the metabolism of other drugs.Their other drug-able properties and ADMET properties are shown in supplementary data (►Tables S3-S5 [Supporting Information]).Overall, the ADMET properties of these compounds indicated that the optimized lupenone derivate and 2,7dimethylnaphtalene had better octanol-water coefficient and better gastrointestinal tract permeability presenting these compounds as potential drug candidates.More so, the toxicity profile of these compounds showed they are relatively non-toxic to vital organs.
Therefore, this study presents lupenone and 2,7-dimethylnaphthalene, as two novel BCE-derived hypolipidemic compounds, as potential agents that take their effect via their stronger binding potentials to ACC and FASN in docking.This study thus provided potential therapeutic drug candidates for the research efforts to treat hyperlipidemia and its associated disease complications, especially since these compounds showed higher affinity for the studied proteins in silico than established standard inhibitors of the proteins firsocostat and C75 trans.One of the possible effector mechanisms of these potential drug agents against ACC and FASN could be based on the premise that lupenone and 2,7-dimethylnaphthalene inhibit ACC and FASN activities by interacting within the phosphopeptide-acceptor of bonded amino acids and dimerization site of these enzymes to prevent their dimerization, 42 since the enzymes are only active in a dimerized form.This interaction is exemplified by the structure of lupenone and 2, 7-dimethylnaphthalene, modeled into the crystal structure of the protein complexes.

Conclusion
To the best of our knowledge, this study was the first to reveal that BCE is a potential source of hypolipidemic agents and presented two new potential hypolipidemic BCE agents, lupenone and 2,7-dimethylnaphthalene, with potential usefulness in dyslipidemia management via ACC and FASN inhibition from natural plant sources.Moreover, these compounds also exhibited promising druggable properties in silico.However, further preclinical, and clinical wet laboratory procedures would be required to validate these outcomes in future studies.

Fig. 6
Fig. 6 Docking structure of different complexes.Analysis of docked pose of (A) acetyl-CoA carboxylase-lupenone complex (1UYS-92158), where the left panel displays the ligand bound at the pocket of the receptor 1UYS and the right panel exhibits the binding pocket residues interacted with the ligand.(B) Fatty acid synthase-2,7-dimethylnaphthalene complex (2PX6-11396), where the left panel displays the ligand bound at the pocket of the receptor 2PX6 and the right panel exhibits the binding pocket residues interacting with the ligand.

Table 1
Results of phytochemical screening for Buchholzia coriacea