Impact of Thoracic Endovascular Repair on Pulsatile Aortic Strain in Acute Type B Aortic Dissection

Abstract Background: The impact of thoracic endovascular aortic repair (TEVAR) on pulsatile aortic strain remains undetermined in patients with Type B aortic dissection (TBAD). Therefore, we quantified pulsatile aortic strain in TBAD patients and control subjects. Methods: We retrospectively analyzed two TBAD patients from our database with cardiac-gated computed tomography angiography imaging available before and after TEVAR and two control subjects (67- and 76-year-old males). Patient 1 (54-year-old female) presented with acute TBAD, and Patient 2 (55-year-old male) had Marfan syndrome and ruptured acute TBAD. Custom-developed software was used to compute aortic length, diameter, and area during the cardiac cycle. Pulsatile strain was calculated as systolic increments of length and circumference divided by corresponding diastolic values. Results: Before TEVAR, pulsatile longitudinal strain of the thoracic aorta was lower in TBAD patients (1.4–1.7%) than in control subjects (2.1–4.5%). After TEVAR, pulsatile longitudinal strain increased proximal to the stent-graft by 65% in the arch of Patient 1 and by 70% in the ascending aorta of Patient 2. Pulsatile circumferential strain was elevated in false lumen patency (4.4–6.2%) compared with thrombosed false lumen (1.4–2.1%) or control subjects (0.9–3.3%). Following TEVAR, circumferential measurements within stented segments were deemed unreliable due to artifacts. Conclusions: TEVAR led to a considerable increase of pulsatile longitudinal strain proximal to the stent-grafts, and TBAD was associated with longitudinally stiffer aortas, which may be part of the pathophysiology of TEVAR-related complications such as retrograde dissection and aneurysmal dilatation. These preliminary data call for larger prospective studies.


Introduction
Thoracic endovascular aortic repair (TEVAR) has been successfully adopted to treat complicated Type B aortic dissection (TBAD) [1]. However, major TEVAR-related complications are widely reported with retrograde or antegrade dissection, typically originating at the proximal or distal end of the device, with rupture being the most lethal [2][3][4]. Unfortunately, the mechanisms of these TEVAR-related complications are mostly unknown. Changes in aortic dynamics during the cardiac cycle, induced by a mismatch between the stent-graft and the aorta, are suggested to play a key role [2,5]. However, little is known about deformations of the dissected aortic wall, and few studies have reported strain after endovascular repair [6].
Biomechanical studies show that current thoracic stent-grafts are about 125 times stiffer than the adult thoracic aorta (55.2 MPa vs. 0.44 MPa) [7,8]. Implantation of such stiff devices may underlie reported cases of TEVAR-induced acute hypertension and cardiac remodeling [9,10]. However, the exact nature of TEVAR-induced local changes in aortic wall strain remains to be determined. While most dynamic imaging studies have focused on aortic area or diameter changes during the cardiac cycle [11][12][13], the importance of longitudinal strain should be not underestimated, as it is predominantly the longitudinal axis that fails in the event of aortic dissection [14][15][16]. During each cardiac contraction, the heart pulls the aortic root downward, resulting in longitudinal strain of about 7-9% in the ascending aorta [15,17]. Implantation of a rigid stent-graft in the proximal descending aorta might stiffen that segment, potentially forcing the ascending aorta and aortic arch to overstretch during each heartbeat, as we observed in a study of eight aneurysm patients managed with TEVAR (unpublished data). Such elevated wall stress has been associated with increased risk of aortic dissection [15] and aneurysm growth [5].
These considerations led us to quantify pulsatile aortic strain in both longitudinal and circumferential directions in acute TBAD patients before and after TEVAR as well as control subjects. For this purpose, we used a semi-automatic technique to post-process cardiac-gated computed tomography angiography (CTA) image data.

Materials and Methods
For this retrospective study, we included patients with acute TBAD enrolled in our imaging database who underwent cardiac-gated CTA before and after TEVAR at the University of Utrecht, The Netherlands, which resulted in a limited number of patients (n = 2). Two patients without thoracic aortic disease served as control subjects ( Table 1). The local ethical review board evaluated the study protocol; formal approval was given, and informed consent was waived.

Patient Population
Patient 1 was a 54-year-old female with acute TBAD and thrombosed false lumen ( Figure 1A). This patient underwent TEVAR using two Bolton Relay (Bolton Medical Inc, Sunrise, FL) stent-grafts ( Figure 1B). The proximal landing zone was just distal to the left common carotid artery, covering the left subclavian artery (LSA), with distal extension 10 cm above the celiac bifurcation. Stent-graft oversizing was 8%. A postoperative cardiac-gated CTA was conducted 3 weeks later, which was used for this study. During 5 years of follow-up, no endoleaks or other stent-graft-related Two Medtronic Captivia (Medtronic Vascular, Santa Rosa, CA) stent-grafts were implanted, with extension distal from the LSA to just proximal to the celiac bifurcation ( Figure 1D). The stent-graft diameter was oversized by 40% considering the ruptured aorta and hypotension [18] found during examination as well as the unavailability of smaller devices in the acute setting in our hospital. The cardiac-gated CTA conducted at the second presentation and 3 days postoperatively were used for this study. After 1 month, CTA imaging revealed a pseudoaneurysm caused by aortic perforation of a proximal bare stent strut, as previously reported [19]. A proximal stent-graft extension was implanted (32-32-164 mm), landing just distal to the left common carotid artery and covering the origin of the LSA. No endoleaks or other stent-graft-related complications occurred during 5 years of follow-up, with complete thrombosis of the false lumen and subsequent positive remodeling of the aorta. Patients 3 and 4 were both males (67-and 76-yearsold) with no visible evidence of thoracic aortic disease and no medical history of aortic surgery or connective tissue disorder.

Workflow from Data Collection to Strain Quantification
The workflow for this study is illustrated in Figure 2 and described stepwise below.
1. Image acquisition and data collection: Basic input data was a dicom set of cardiac-gated CTA images obtained during eight phases of the cardiac cycle. These dynamic images were obtained with a 256-row multislice CT system (Philips Medical System, Best, The Netherlands) and imported into 3Mensio software (3Mensio Medical Imaging, Bilthoven, The Netherlands) for analysis. A three-dimensional (3-D) scan volume was acquired during all eight phases, for which maximum (end-systolic) and minimum (end-diastolic) measurements were acquired. Patients were included in the study only if image acquisition was accomplished successfully and image quality was considered adequate. Pixel spacing and slice thickness were similar for all patients and are described in Supplemental Table 1 (see supplemental Table 1 at http://dx.doi. complications were noted. Patient 2 was a 55-year-old male with Marfan syndrome with acute TBAD and patent false lumen from the mid-descending aorta to the iliac arteries. This patient was initially managed with medical therapy. Six days after the first acute event, he developed a contained rupture of the proximal descending aorta and was managed with emergency TEVAR ( Figure 1C).  The thoracic aorta was segmented at each phase, and three-dimensional reconstructed models were computed. Panel C. Center lumen lines were computed. Panels D and E. Longitudinal and circumferential segments were identified using side branch origins as anatomical landmarks, resulting into (Panel D) longitudinal segments (ascending aorta (purple), aortic arch (green), and descending aorta (red)) and (Panel E) circumferential levels (sinotubular junction (STJ), just before the origin of the brachiocephalic trunk (BCT), left subclavian artery (LSA), 10 cm (LSA + 10 cm) and 20 cm (LSA + 20 cm) distal to the left subclavian artery, and celiac trunk (CT)). Lengths, areas, and diameters were then calculated by a custom-developed script [21].
LSA, 10 and 20 cm distal to the LSA, and celiac trunk ( Figure 2E). Diameters were measured in 360° by calculating the distance between all points constituting the section of interest perpendicular to the center lumen line and then extracting the end-diastolic and end-systolic values, as previously described [12,13]. Aortic areas were measured by triangulating the particular section and summing the area of all obtained triangles. Pulsatile circumferential strain was computed as the difference between end-systolic and end-diastolic circumference divided by end-diastolic circumference.

Intra-and Inter-Observer Variability
Length and area measurements were acquired twice by the same investigator (C.T.) and blindly repeated by another investigator (F.N.) to allow for intra-and inter-observer variability.

Statistical Analysis
Data were analyzed with SPSS 22.0 (SPSS, Chicago, IL). Continuous data are presented as mean ± standard deviation (SD) unless stated otherwise, and categorical data are given as counts (percentage). Intra-and inter-observer variability were calculated according to Bland and Altman. Data were considered normally distributed based on skewness and kurtosis Z-values between -1.96 and 1.96, a Shapiro-Wilk test p > 0.05, and visualization of approximately normally distributed data with histograms. p < 0.05 were considered statistically significant.

Results
Patient and procedural characteristics are shown in Table 1. The median interval from TEVAR to postoperative cardiac-gated CTA imaging was 0.4 months (range, 0.1-0.7).

Aortic Length and Pulsatile Longitudinal Strain
Aortic length and pulsatile longitudinal strain before and after TEVAR are shown in Table 2. In Patient 1, the end-systolic length of the descending aorta decreased following TEVAR, while the ascending aorta org/10.12945/j.aorta.2017. 16.042.sup.02). For enhanced vessel contrast, each patient received 90-150 mL non-ionic contrast medium (Iopromide, Schering, Berlin, Germany) followed by a 60-mL saline chaser bolus. 2. Image segmentation: An imaging workstation was created to post-process the four-dimensional (4-D) image sets. Dedicated ITK-Snap software [20] was used to segment the thoracic aortic geometries of each phase both pre-and post-TEVAR. This software implements a 3-D active contour segmentation method, called snake evolution, which works on the closed surface of the aortic lumen. In addition, 3-D reconstruction of the different levels of interest was conducted for each phase ( Figure 2B).  Figure 2D). For each patient, pulsatile longitudinal strain was calculated as the difference between systolic and diastolic lengths divided by diastolic length. 5. Pulsatile circumferential strain: Aortic diameters and areas were measured at six different levels: the sinotubular junction, brachiocephalic trunk,

Aortic Diameter and Pulsatile Circumferential Strain
End-systolic diameter and circumferential strain are shown in Table 3. Overall, smaller diameters of the true lumen were observed in TBAD patients than in control subjects (from 10 cm distal to the LSA to the celiac trunk). Figure 4 illustrates pulsatile circumferential strain before TEVAR, which ranged from 0.9% to 3.3% and was comparable among Patient 1 and the two control subjects. However, Patient 2, who presented with acute TBAD and patent false lumen, showed considerably higher circumferential strain at sections of the patent false lumen (4.4% at 20 cm distal to the LSA and 6.2% at the celiac trunk).
After TEVAR, the true lumen expanded in sections adjacent to the stent-graft (brachiocephalic trunk and celiac trunk). Pulsatile circumferential strain also increased in sections adjacent to the stent-grafts, except for the distal adjacent section in Patient 2 (celiac trunk with patent false lumen), which decreased considerably (6.2% before TEVAR vs. 2.5% after TEVAR). Diameter and area measurements within stented sections were deemed unreliable due to stent artifacts in the segmentations and were therefore excluded from this analysis. and arch elongated. Patient 2 showed similar shortening of the descending aorta with elongation of the ascending aorta and arch after TEVAR.
Pulsatile longitudinal strain before and after TE-VAR in the two acute TBAD patients are illustrated in Figure 3. Before TEVAR, pulsatile longitudinal strain throughout the thoracic aorta ranged from 1.5% to 9.9% in the two TBAD patients and from 1.7% to 14.0% in the two control subjects, with mean pulsatile longitudinal strain of the total thoracic aorta of 1.9 ± 0.5% and 3.3 ± 1.2%, respectively. In three of the four patients, pulsatile longitudinal strain was highest in the ascending aorta (9.9-14.0%), decreasing downstream along the arch (3.9-9.6%) and descending aorta (1.7-4.9%).
After TEVAR, an increase of pulsatile longitudinal strain was observed proximal to the stent-graft in both TBAD patients (65% increase in the arch of Patient 1, and 70% increase in the ascending aorta of Patient 2). In the descending aorta, Patient 1, with a stent-graft length of 174 mm, showed increased longitudinal strain (+60%) following TEVAR. By contrast, Patient 2, with a longer stent-graft length of 235 mm, showed decreased longitudinal strain (-65%) in the descending aorta after TEVAR.   Values are shown as mean ± SD, where appropriate. BCT = brachiocephalic trunk; CT = celiac trunk; LSA = left subclavian artery; STJ = sinotubular junction; TEVAR = thoracic endovascular aortic repair.

Intra-and Inter-Observer Variability
For area changes, the intra-observer repeatability coefficient (RC) was 22.85 mm 2 . Mean differences between pre-and post-TEVAR area changes were smaller than the RCs, and linear regression analysis was non-significant (p = 0.31). Inter-observer repeatability showed an RC of 17.88 mm 2 , and mean differences between pre-and post-TEVAR area changes were smaller than the RCs. Linear regression analysis was non-significant (p = 0.46). These results indicate good intra-and inter-observer agreement.
For length changes, the RC for intra-observer repeatability was 2.78 mm, and mean differences between pre-and post-TEVAR length changes were smaller than the RCs. Linear regression analysis was non-significant (p = 0.70). The RC for interobserver repeatability was 2.84 mm. Mean differences between pre-and post-TEVAR areas were smaller than the RCs, and linear regression analysis was non-significant (p = 0.64). Again, these results indicate good intra-and inter-observer agreement.

Discussion
In both TBAD patients, TEVAR was followed by elevated pulsatile longitudinal strain proximal to the stent-graft. Moreover, TBAD patients showed lower pulsatile longitudinal strain of the total thoracic aorta before TEVAR compared with control subjects. Regarding pulsatile circumferential strain, Patient 1, who presented with patent false lumen, showed higher strain of the true lumen when compared with Patient 2, who had a thrombosed false lumen. Pulsatile circumferential strain in control subjects ranged from 0.9% to 3.3% and was consistent along the thoracic aorta, which agrees with previous literature [22].
After TEVAR, we found increased pulsatile longitudinal wall strain proximal to the stent-grafts in both TBAD patients. Other authors suggest that increased pulsatile wall stress is associated with aortic wall fatigue, resulting in aneurysmal dilatation and increased risk of new entry tears at the proximal or distal end of the stent-graft or rupture [2][3][4][5]. Patients with fragile aortic walls, such as those with aortic dissection, are at particular risk for such sudden strain changes. In our study, Patient 2 developed aortic perforation due to a stent strut. In this patient, we observed a considerable increase in circumferential strain (1.9% vs. 3.6%) at the LSA after the initial TEVAR procedure. This elevated strain may have triggered the strut perforation. To better understand the pathophysiology of such complications, further studies are warranted to investigate pulsatile strain changes following TEVAR.
Pulsatile longitudinal strain was lower in TBAD patients than in older-aged control subjects (1.4-1.7% vs. 2.1-4.5%), even though aging is associated with aortic stiffening [8,22]. This observation suggests that aortic stiffening is a risk factor for aortic dissection, although this remains to be confirmed by larger studies. Moreover, in Patient 2, we observed that stent-grafting of a longer segment was associated with a decrease in longitudinal strain, stiffening the descending aorta and potentially exerting adverse effects on cardiac function and geometry [10].
We noticed markedly high pulsatile circumferential strain at the level of patent false lumen in Patient 1. This was in contrast to the control subjects and Patient 2, who had a thrombosed false lumen. This observation emphasizes the potential role of variables such as dissection flap motion and thrombosis on prognosis. It also sheds light on differences between acute and chronic TBAD and underscores the importance of dynamic imaging to assess these risk factors and correctly size stent-grafts. Moreover, a substantial pulsatile circumferential strain may have implications for future device selection, as patients with more compliant aortas may benefit from more compliant stent-grafts to minimize mismatch.
Aortic stiffness is reportedly higher in Marfan patients [23]. We did not observe this in our study, most likely because our Marfan patient (Patient 2) presented with acute TBAD complicated by rupture. The movement of the dissection flap elevated the pulsatile circumferential strain before TEVAR. In addition, this patient was younger than the two control subjects, and younger age is associated with higher strain [22].
To continuously improve treatment outcomes of TBAD, it is important to obtain data on the biomechanical behavior of dissected aortas. In particular, promising advancements in computational fluid dynamics offer unique insights into complex vascular pathologies [8]. However, such computational techniques depend on clinically measured data to serve References as boundary conditions, which are only sparsely available for TBAD. Thus, this study provides preliminary data on dissection flap strain.
The main limitation of this study is the small cohort, as we were only able to include two TBAD patients and two control subjects. Therefore, our results cannot be generalized and should be considered descriptive. Moreover, we were unable to report circumferential strain within stented segments due to artifacts. More systematic studies are needed to elucidate the effects of aortic stent-grafts on the physiologic function of the aorta. Finally, with advancements in medical imaging, pixel spacing and the number of equidistant time steps during the cardiac cycle could be improved and should be further investigated.
In conclusion, this study represents an initial investigation on pulsatile aortic strain in patients with acute TBAD before and after TEVAR. We observed considerably higher pulsatile circumferential strain in a case of patent false lumen compared with thrombosed false lumen. After TEVAR, both TBAD patients showed increased pulsatile longitudinal strain proximal to the stent-graft, which is known to be associated with an increased risk of aortic dissection. These observations may motivate future research to assess the biomechanical impact of TEVAR in order to continuously improve treatment outcomes for patients with TBAD.