Case series: Nocardiosis of the brain and lungs

Abstract Localized and multisystem nocardiosis is an opportunistic disease that occurs commonly in immunocompromised patients. Rarely, it is also seen in immunocompetent individuals. The lungs and brain are commonly involved. Typical, but nonspecific, findings are often seen on imaging and the presence of concomitant lesions in these two systems often suggests this diagnosis. We report two cases of cerebral and pulmonary involvement by nocardiosis.

The laboratory investigations revealed a raised white blood cell count and reduced platelet count, total proteins, and albumin. The blood urea nitrogen, creatinine, random blood sugar, serum electrolytes, and liver function tests were within normal limits. The chest radiograph was also normal.
MRI of the brain revealed well-deÞ ned ring-enhancing lesions in both the frontal subcortical regions [ Figure 1A], with signiÞ cant surrounding edema. The lesions displayed central hyperintensity, with a peripheral low signal intensity rim on the T2W images [ Figure 1B]. On T1W images, the central contents were hypointense and the walls showed intermediate signal intensity. Similar lesions were also seen in both occipital regions and the right temporal region. Minimal meningeal enhancement was also seen in the right occipital and high frontoparietal regions bilaterally. A diagnosis of multiple brain abscesses was made.
CT scan of the thorax [ Figure 2] revealed consolidation and collapse with necrosis in the superior segment of the right upper lobe. Small areas of alveolar opaciÞ cation were seen in both the lungs. A small left pleural eff usion and a pericardial eff usion were also seen.
CT scan of the abdomen [ Figure 3] revealed multiple loculated abscesses within the muscular planes of the thoracoabdominal wall as well as in the left parapsoas region and the right iliacus, left obturator externus, and posterior spinal muscles. Collections were also seen within the mesentry and in the right paracardiac region.
CT-guided aspiration from the abscess in the posterior spinal muscle was performed; the aspirate grew Nocardia on culture. Pus aspirated from the intracranial lesion under local anesthesia also grew nocardia. The organism was identiÞ ed upto the genus level; however, speciation could not be done.
The patient was put on appropriate treatment.

Case 2
A 60-year-old man who had had diabetes and hypertension for the last 15 years presented with a history of right-sided weakness which progressed slowly over a period of 2 weeks. A week before presentation he had also developed headache, vomiting, and diffi culty in swallowing. At presentation, he had loss of speech followed by altered sensorium. The patient had also been having cough with minimal sputum off and on and loss of weight for the last 3 months.
The patient had a focal lesion on a brain MRI, along with a left pleural eff usion on a chest radiograph, and had been treated for tuberculosis. When he was Þ nally seen in our hospital, the patient was afebrile and had a raised pulse rate and blood pressure. The liver was mildly enlarged. Respiratory and cardiovascular system examination was unremarkable. He had altered sensorium, with aphasia and complete weakness of the right side of the body (grade 0/5 power in both upper and lower limbs). The plantar was upgoing on the right side and there was gaze preference to the left . Fundus examination revealed a few microaneurysms at the macula, a few cott on wool spots, and mild arteriolar att enuation in both the eyes.
The laboratory examination revealed normal red blood cell and renal function parameters, with normal sodium and potassium levels. The total leucocyte count was reduced.
Contrast-enhanced CT of the head [ Figure 4A] revealed conglomerate, ring-enhancing lesions in the left parietal lobe, with signiÞ cant surrounding edema and contralateral shift . There was no abnormal meningeal enhancement. Contrast-enhanced MRI of the brain revealed perpherally enhancing conglomerate lesions in the left parietal lobe [ Figure 4B], with signiÞ cant surrounding edema. These lesions showed central hyperintense signals with low signal intensity walls on the T2W images [ Figure 4C]. On T1W images, the central contents appeared hypointense. USG of the abdomen showed a small right pleural eff usion and hepatomegaly. Contrast-enhanced CT scan of the chest revealed necrotic lymph nodes in the left hilar region [ Figure 5A] and consolidation in the left lung [ Figure 5B]. Bronchial aspiration did not reveal the presence of acid fast bacilli (AFB). Fungal smear was also negative.
The patient showed no response to antituberculous treatment. A temporoparietal craniotomy was performed and the cyst was excised. The histopathology revealed nocardiosis, which was identiÞ ed up to the genus level; speciation was not done.

Discussion
Nocardiosis, caused by gram-positive, weakly acid-fast, Þ lamentous aerobic actinomycetes, is an opportunistic infection. Nocardia asteroides is the most common species that infects humans. An increasing number of cases are being reported in immunocompetent individuals without predisposing factors. [1] Involvement of the lungs (75-80%) and the skin is the most common form of presentation, but virtually any organ system may be involved. [2][3][4] The central nervous system is involved in approximately 44% of cases, usually in patients having pulmonary infection, as seen in both our cases. [5] When there is involvement of two or more noncontiguous organs, with or without CNS involvement, the disease is said to be disseminated. The incidence of disseminated disease is 25-40%. [6] Both our cases had disseminated disease.
Imaging Þ ndings are not speciÞ c for nocardiosis. In the chest, it can present as pulmonary consolidation, irregular nodular parenchymal densities, solitary irregular lung mass, interstitial reticular pattern, pleural effusion, or lymphadenopathy, but none of these is a speciÞ c diagnostic feature. [7] Both our patients had areas of consolidation in the lungs.
The imaging Þ ndings in the brain depend on the stage of the infectious process at the time of imaging; this may vary from cerebritis to frank abscess formation. In the abscess stage, the necrotic debris accumulates centrally, while the collagenous capsule is being formed. The proteinaceous, necrotic debris has signal intensity higher than that of the CSF on T1W and turbo inversion-recovery (IR) images, with a moderate degree of brain edema. On T1W images, the abscess capsule stands out against the necrotic center and surrounding edema as an isointense to slightly hyperintense ring. On T2W images, the ring is consistently hypointense. This hyperintense capsule may either be due to capsular hemorrhage, the paramagnetic methemoglobin causing increased signal on T1W images, or due to an abundance of free radicals in the macrophages in the abscess capsule. The activity of the macrophages is highest in the late cerebritis and early abscess phases, at which time the capsule shows marked hypointensity on T2W images. [8] Ring enhancement is seen, which may persist for upto 8 months aft er treatment and therefore should not be interpreted as failure of treatment. Reliable signs of good response to treatment are shrinkage of the necrotic center and decrease in capsular hypointensity on T2W images. appropriate clinical sett ings, especially in patients not responding to antituberulous treatment. Skin involvement occurring along with lung and brain lesions is commonly seen in nocardiosis and is a pointer towards the diagnosis. Multidrug medical therapy, with surgical drainage of the abscess, is advocated in the disseminated form of the disease.
The diagnosis is confirmed by direct microscopy and culture. [1] Demonstration of marked polymorphonuclear leukocytes in the pus, in the absence of regional lymph node enlargement, is said to be characteristic. [6] There are only three previous reports on disseminated nocardiosis in patients with chronic ITP. [2,3,6] These patients need to be treated with intensive combination chemotherapy, along with slow tapering off of the steroids. [9][10][11] The drug of choice is co-trimoxazole [3,4] which can be given alone or in combination with one or more of the following antimicrobial drugs: imipenim, amikacin, ampicillin, third-generation cephalosporins, fluoroquinolones, or minocylines; cotrimoxazole is usually given by the intravenous route in cerebral nocardiosis. The usually recommended duration of therapy is for 6-12 months. [3] The decision to intervene surgically varies from case to case. [3] Our first patient, who was a known case of ITP and had disseminated nocardial infection, showed initial improvement in the skin and lung lesions, but there was no improvement in the cerebral lesions. As there was subsequent neurological deterioration, the right frontal brain abscess was surgically excised under intensive platelet cover. He initially showed improvement but eventually succumbed to his illness aft er 4 months of treatment.
Our second patient underwent a temporoparietal craniotomy, with excision of the abscess, as he was not responding to antituberculous treatment and a histopathological diagnosis had to be obtained. He did well postoperatively for a period of one month, but then he too succumbed to his illness.
An overall mortality of 40-60% has been reported in disseminated nocardiosis. [4] When patients present with comcomitant brain and lung lesions, the differential diagnoses to be kept in mind include tuberculosis, neoplastic disease, and nocardiosis. Nocardiosis can aff ect both immunocompromised and immunocompetent individuals. As the imaging Þ ndings are nonspeciÞ c and the causative organism is diffi cult to isolate, diagnosis is often a problem and hence it is important to have a high index of suspicion in the Source of Support: Nil, Confl ict of Interest: None declared.