Replications of fundamental research models in ultra high dilutions 1994 and 2015 – update on a bibliometric study

Peter Christian Endler; Paolo Bellavite; Leoni Bonamin; Tim Jäger; Sintia Mazon

doi:10.1016/j.homp.2015.10.003

Homeopathy, Table of Contents

Homeopathy 2015; 104(04): 234-245
DOI: 10.1016/j.homp.2015.10.003

Original Paper

Replications of fundamental research models in ultra high dilutions 1994 and 2015 – update on a bibliometric study

Peter Christian Endler

¹Interuniversity College for Health and Development Graz/Castle of Seggau, Austria

,

Paolo Bellavite

²University of Verona, Italy

,

Leoni Bonamin

³Universidade Paulista, Sao Paulo, Brazil

,

Tim Jäger

⁴University of Witten/Herdecke, Germany

⁵Institute of Complementary Medicine IKOM, University of Bern, Switzerland

⁶University of Freiburg, Germany

,

Sintia Mazon

¹Interuniversity College for Health and Development Graz/Castle of Seggau, Austria

› Author Affiliations

Abstract

Introduction: This paper focuses exclusively on experimental models with ultra high dilutions (i.e. beyond 10⁻²³) that have been submitted to replication scrutiny. It updates previous surveys, considers suggestions made by the research community and compares the state of replication in 1994 with that in 2015.

Methods: Following literature research, biochemical, immunological, botanical, cell biological and zoological studies on ultra high dilutions (potencies) were included. Reports were grouped into initial studies, laboratory-internal, multicentre and external replications. Repetition could yield either comparable, or zero, or opposite results. The null-hypothesis was that test and control groups would not be distinguishable (zero effect).

Results: A total of 126 studies were found. From these, 28 were initial studies. When all 98 replicative studies were considered, 70.4% (i.e. 69) reported a result comparable to that of the initial study, 20.4% (20) zero effect and 9.2% (9) an opposite result. Both for the studies until 1994 and the studies 1995–2015 the null-hypothesis (dominance of zero results) should be rejected. Furthermore, the odds of finding a comparable result are generally higher than of finding an opposite result. Although this is true for all three types of replication studies, the fraction of comparable studies diminishes from laboratory-internal (total 82.9%) to multicentre (total 75%) to external (total 48.3%), while the fraction of opposite results was 4.9%, 10.7% and 13.8%. Furthermore, it became obvious that the probability of an external replication producing comparable results is bigger for models that had already been further scrutinized by the initial researchers.

Conclusions: We found 28 experimental models which underwent replication. In total, 24 models were replicated with comparable results, 12 models with zero effect, and 6 models with opposite results. Five models were externally reproduced with comparable results. We encourage further replications of studies in order to learn more about the model systems used.

Keywords

Review - Basic research - Homeopathy - Ultra high dilution - Replication

Full Text

References

References
1 Persson W.M., Ginsberg A.S. Die Einwirkung von Mikrodosen homöopathischer Arzneimittel (chemischer oder pflanzlicher) auf die Fermente Urease und Diastase. The action of microdoses of homeopathic remedies (of chemicals and plants) on the enzymes urease and diastase Dt Z Hom 1932; 11: 97-106.
2 Bluth M. In-vitro-Forschung mit homöopathischen Potenzen. Ein systematischer Review und eigene Versuche mit zellfreien Systemen. In-vitro-research on homeopathic potencies. A systematic review and own experiments with cell-free systems. Thesis Berlin: Institut für Sozialmedizin, Epidemiologieund Gesundheitsökonomie; Charité-Universitätsmedizin.; 2005.
3 Boyd W.E. Biochemical and biological evidence of the activity of high potencies. Br Hom J 1954; 44: 7-44.
4 Harisch G., Dittmann J. Untersuchungen zur Wirkung von Ubichinon Injeel und Injeel forte mit zellfreien Systemen. Investigations of the result of Ubichinon Injeel and Injeel forte with cell-free systems Biol Med 1997; 26: 99-104.
5 Harisch G., Dittmann J. Aktivitätsbestimmungen der sauren Phosphatase in Gegenwart von Ubichinon-Einzelpotenzen und Ubichinon-Potenzmischungen. Determination of activity of acid phosphatase in presence of single potencies and potency mixtures of ubiquinone Biol Med 1999; 28: 188-194.
6 Harisch G., Dittmann J. Unterschiedlicher Einfluß von cAMP-Potenzen und cAMP-Verdünnungen am Beispiel verschiedener Enzymsysteme. Different influence of potencies and dilutions of cAMP exemplified on several enzyme systems Biol Med 1998; 27: 55-62.
7 Harisch G., Dittmann J. Aktivitätsbestimmung der sauren Phosphatase in Gegenwart von cAMP-Einzelpotenzen und cAMP-Potenzmischungen. Determination of acitivity of acid phosphatase in presence of single potencies and potency mistures of cAMP Biol Med 1999; 28: 4-8.
8 Sukul N.C., Sukul A., Sinhababu S.P. Potentized mercuric chloride and mercuric iodide enhance alpha-amylase activity in vitro . Homeopathy 2002; 91: 217-220.
9 Carmine T.C. Results of high potencies of tumour necrosis factor alpha on H₂O₂ production in cultured neuroblastoma cells by enhanced luminol-dependent chemiluminescence (ECL): a possible system for investigating the biological significance of homeopathic high potencies. Br Hom J 1997; 86: 67-72.
10 Herberth G., Pison U. Homöopathische Arzneimittel in zellbiologischen Systemen. [Homeopathic remedies in cell-biological systems] In: Albrecht H., Frühwald M. (eds). Jahrbuch der Karl und Veronika Carstens-Stiftung. 1999. Essen: Band 5 (1998); 77-95.
11 Graviou E., Biron M.A. Action d’une 15e CH de sulphate de cuivre sur la culture des Chlorella vulgaris . Ann Hom Fr 1971; 7: 539-548.
12 Moss V.A., Roberts J.A., Simpson H.K.L. The result of copper sulphate on the growth of the alga Chlorella. Br Hom J 1977; LXVI/3: 169-176.
13 Kolisko L. Physiologischer und physikalischer Nachweis der Wirksamkeit kleinster Entitäten bei sieben Metallen. Dornach: Goetheanum Verlag.; 1926.
14 Pongratz W., Endler P.C. Reappraisal of a classical botanical experiment in ultra high dilution research. In: Endler P.C., Schulte J. (eds). Ultra High Dilution: Physiology and Physics. 1994. Dordrecht: Kluwer Academic Publishers; 121-128.
15 Pongratz W., Nograsek A., Endler P.C. Highly diluted agitated silver nitrate and wheat seedling development. Result kinetics of a process of successive agitation phases. In: Schulte J., Endler P.C. (eds). Fundamental Research in Ultra High Dilution and Homeopathy. 1998. Dordrecht: Kluwer Academic Publishers; 155-187.
16 Betti L., Brizzi M., Nani D., Peruzzi M. Result of high dilutions of Arsenicum album on wheat seedlings from seed poisoned with the same substance. Br Hom J 1997; 86: 86-89.
17 Brizzi M., Nani D., Peruzzi M., Betti L. Statistical analysis of the result of high dilutions of arsenic in a large dataset from a wheat germination model. Br Hom J 2000; 89: 63-67.
18 Brizzi M., Lazzarato L., Nani D., Borghini F., Peruzzi M., Betti L. A biostatistical insight into the As₂O₃ high dilution results on the rate and variability of wheat seedling growth. Res Complement Med – Forsch Komplementärmed 2005; 12: 277-283.
19 Nani D., Brizzi M., Lazzarato L., Betti L. The role of variability in evaluating ultra high dilution results: considerations based on plant model experiments. Forsch Komplementärmed 2007; 14 (05) 301-305.
20 Brizzi M., Elia V., Trebbi G., Nani D., Peruzzi M., Betti L. The efficacy of ultramolecular aqueous dilutions on a wheat germination model as a function of heat and aging-time. Evid Based Complement Alternat Med 2011; Article ID 696298.
21 Lahnstein L., Binder M., Thurneysen A. et al. Isopathic treatment results of arsenicum album 45x on wheat seedling growth – further reproduction trials. Homeopathy 2009; 98: 198-207.
22 Binder M., Baumgartner S., Thurneysen A. The results of a 45x potency of arsenicum album on wheat seedling growth – a reproduction trial. Res Complement Med – Forsch Komplementärmed 2005; 12: 284-291.
23 Pfleger A., Hofäcker J., Scherer-Pongratz W., Lothaller H., Reich C., Endler P.C. The result of extremely diluted agitated gibberellic acid (10e-30) on wheat stalk growth – a two researcher pilot study. Complement Ther Med 2011; 19: 164-169.
24 Endler P.C., Matzer W., Reich C. et al. Seasonal variation of the result of extremely diluted agitated gibberellic acid (10e-30) on wheat stalk growth: a multiresearcher study. ScientificWorldJournal 2011; 11: 1167-1678.
25 Hribar-Marko S., Graunke H., Scherer-Pongratz W., Lothaller H., Endler P.C. Prestimulation of wheat seedlings with gibberellic acid followed by application of an agitated high dilution of the same hormone. Int J High Dilution Res 2013; 12 (42) 26-39.
26 Hartung H., Schiestl S., Matzer W., Endler P.C. Wheat germination (20 hrs) and extremely diluted gibberellic acid (10e-30): explorative experiments on a fundamental homoeopathy research model. Eur J Integr Med 2010; 2: 224 [Abstract].
27 Kiefer P., Matzer W., Schiestl S. et al. Wheat germination and highly diluted agitated gibberellic acid (10⁻³⁰) – a multi researcher study. Int J High Dilution Res 2012; 11 (39) 45-59.
28 Scherr C., Simon M., Spranger J., Baumgartner S. Results of potentized substances on growth rate of the water plant L. Complement Ther Med 2009; 17 (02) 63-70.
29 Majewsky V., Scherr C., Arlt S.P. et al. Reproducibility of results of homeopathically potentized gibberellic acid on the growth of Lemna gibba L. in a randomized and blinded bioassay. Homeopathy 2014; 103: 113-126.
30 Baumgartner S., Doesburg P., Scherr C., Andersen J.O. Development of a biocrystallisation assay for examining results of homeopathic preparations using cress seedlings. Evid Based Complement Alternat Med 2012; 1-14.
31 Doesburg P., Andersen J.O., Scherr C., Baumgartner S. Replication of specific results of a stannium metallicum 30x preparation in a cress seedling/biocrystallisation test system. Proceedings 2nd HRI conference, Rome 2015; 32.
32 Davenas E., Beauvais F., Amara J. et al. Human basophil degranulation triggered by very dilute antiserum against IgE. Nature 1988; 333: 816-818.
33 Benveniste J., Davenas E., Ducot B., Cornillet B., Poitevin B., Spira A. L’agitation de solutions hautement diluées n′indit pas d’activité biologique spécifique. Agitating highly diluted solutions does not induce specific biological activity C R l’Acad Paris 1991; 312: 461-466.
34 Ovelgönne J.H., Bol A.W.J.M., Hop W.C.J., van Wijk R. Mechanical agitation of very dilute antiserum against IgE has no result on basophil staining properties. Experientia 1992; 48: 504-508.
35 Hirst S.J., Hayes N.A., Burridge J., Pearce F.L., Foreman J.C. Human basophil degranulation is not triggered by very dilute antiserum against human IgE. Nature 1993; 366: 525-527.
36 Poitevin B., Aubin M., Benveniste J. Approche d’une analyse quantitative de l’effet d’apis mellifica sur la dégranulation des basophiles humains in vitro . Innov Technol Biol Med 1986; 7 (01) 64-68.
37 Poitevin B., Davenas E., Benveniste J. In vitro immunological degranulation of human basophils is modulated by lung histamine and Apis mellifica . Br J Clin Pharmacol 1988; 25 (04) 439-444.
38 Sainte-Laudy J., Belon P. Biological activity of ultra low doses. II. Result of ultra low doses of histamine on human basophil degranulation triggered by anti-IgE. In: Doutremepuich C. (eds). Ultra Low Doses. 1991. London, Washington: Taylor & Francis; 139-144.
39 Sainte-Laudy J., Belon P. Inhibition of human basophil activation by high dilutions of histamine. Agents Actions 1993; 38: 525-527.
40 Sainte-Laudy J., Belon P. Analysis of immunosuppressive activity of serial dilutions of histamine on human basophil activation by flow cytometry. Inflamm Res 1996; 45: 33-34.
41 Sainte-Laudy J., Belon P. Application of flow cytometry to the analysis of the immunosuppressive result of histamine dilutions on human basophil activation: result of cimetidine. Inflamm Res 1997; 46: 27-28.
42 Sainte-Laudy J., Belon P. Improvement of flow cytometric analysis of basophil activation inhibition by high histamine dilutions. A novel basophil specific marker: CD 203c. Homeopathy 2006; 95 (01) 3-8.
43 Sainte-Laudy J., Boujenaini N., Belon P. Confirmation of biological results of high dilutions. Results of submolecular concentrations of histamine and 1-, 3- and 4-methylhistamines on human basophil activation. Inflamm Res 2008; 57 (01) S01-S02.
44 Belon P., Cumps J., Ennis M. et al. Histamine dilutions modulate basophile activation. Inflamm Res 2004; 53: 181-188.
45 Brown V., Ennis M. Flow-cytometric analysis of basophil activation: inhibition by histamine at conventional and homeopathic concentrations. Inflamm Res 2001; 50: 47-48.
46 Lorenz I., Schneider E.M., Stolz P., Brack A., Strube J. Sensitive flow cytometric method to test basophil activation influenced by homeopathic histamine dilutions. Forsch Komplementärmed Klass Naturheilkd 2003; 10: 316-324.
47 Chirumbolo S., Brizzi M., Ortolani R., Vella A., Bellavite P. Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study. Inflamm Res 2009; 58: 755-764.
48 Guggisberg A.G., Baumgartner S., Tschopp C.M., Heusser P. Replication study concerning the results of homeopathic dilutions of histamine on human basophil degranulation in vitro . Complement Ther Med 2005; 13: 91-100.
49 Wälchli C., Baumgartner S. Flow cytometric analysis of the inhibition of human basophil activation by histamine high dilutions – a replication study. Int J High Dilution Res 2012; 11 (40) 172-173.
50 Sainte-Laudy J. Stimulatory result of high dilutions of histamine on activation of human basophils induced by anti-IgE. Inflamm Res 2001; 50: 63-64.
51 Lorenz I., Schneider E.M., Stolz P., Brack A., Strube J. Influence of the diluent on the result of highly diluted histamine on basophil activation. Homeopathy 2003; 92: 11-18.
52 Colas H., Aubin M., Picard P., Lebecq J.C., Bastide J.M. Inhibition du test de transformation lymphoblastique (TTL) à la phytohémagglutinine (PHA) par Phytolacca americana en dilutions homéopathiques. Inhibition of lymphoblast transformation test (LTT) by phythemagglutinine (PHA) through Phytolacca americana in homeopathic dilutions Hom Franc 1984; 72: 219-224.
53 Bildet J., Dupont H., Aubin M. et al. Action in vitro de dilutions infinitesimales de Phytolacca americana sur la transformation lymphoblastiques (TTL) à la phytohémagglutininec. In vitro action of infinitesimale dilutions of Phytolacca americana on the transformation of lymphoblasts by phytohemagglutinine Hom Franc 1984; 72: 225-230.
54 Francis A.J., Anderson D., Fisher P. Further studies of the ‘adaptive’ repair response in human lymphocytes after treatment with MNNG. Environ Meloc Mutagen 1990; 15 (17) 69 [Abstract].
55 Anderson D., Edwards A.J., Fisher P., Lovell D.P. Statistical analysis of adaptive response in sister chromatid exchanges in human lymphocytes after treatment with very low and extremely low doses of N-methyl-N′-nitro-N-nitrosoguanidine using a study design to control variability. Br Hom J 1999; 88 (01) 7-16.
56 Cristea A., Nicula S., Darie V. Pharmacodynamic results of very high dilutions of belladonna on the isolated rat duodenum. In: Bastide M. (eds). Signals and Images. 1997. Dordrecht: Kluwer Academic Publishers; 161-170.
57 Schmidt F., Süss W.G., Nieber K. In-vitro Testung von homöopathischen Verduennungen. Biol Med 2004; 1: 32-37.
58 Radau K. Materialwissenschaftliche Untersuchungen an pharmazeutischen Hilfsstoffen und ihre Bedeutung für die Herstellung homöopathischer Arzneimittel. Thesis Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig.; 2004.
59 Michael S. Untersuchungen zur Wirkung von homöopathischen Verdünnungen am isolierten Ileum der Ratte. Thesis Institut für Pharmazie, Universität Leipzig.; 2004.
60 Endler P.C., Pongratz W., van Wijk R., Kastberger G., Haidvogl M. Results of highly diluted succussed thyroxin on metamorphosis of highland frogs. Berl J Res Hom 1991; 1: 151-160.
61 Zausner C., Lassnig H., Endler P.C. et al. Die Wirkung von homöopathisch zubereitetem Thyroxin auf die Metamorphose von Hochlandamphibien. Ergebnisse einer multizentrischen Kontrollstudie. Perfusion 2002; 17: 268-276.
62 Welles S.U., Suanjak-Traidl E., Weber S. et al. Pretreatment with thyroxine (10e-8) and the result of homeopathically prepared thyroxin (10e-30) on highland frogs – a multi-researcher study. Res Complement Med – Forsch Komplementärmed 2007; 14: 353-357.
63 Guedes J.R.P., Ferreira C.M., Guimaraes H.M.B., Saldiva P.H.N., Capelozzi V.L. Homeopathically prepared dilution of Rana catesbeiana thyroid glands modifies its rate of metamorphosis. Homeopathy 2004; 93: 132-137.
64 Guedes J.R., Carrasco S., Ferreira C.M. et al. Ultra high dilution of triiodothyronine modifies cellular apoptosis in Rana catesbeiana tadpole tail in vitro . Homeopathy 2011; Oct 100 (04) 220-227.
65 Harrer B. Replication of an experiment on extremely diluted thyroxine and highland amphibians. Homeopathy 2013; Jan 102 (01) 25-30 http://10.1016/j.homp.2012.09.003.
66 Harrer B., Endler P.C. Highland amphibians and extremely diluted thyroxine – pre-experiment storage at low temperature (4°C). 2013. HAR/A, 1–2 www.ResProceedingsIUC.net.
67 Endler P.C., Pongratz W., Smith C.W., Schulte J. Non-molecular information transfer from thyroxin to frogs with regard to ‘homoeopathic’ toxicology. Vet Hum Toxicol 1995; 37 (03) 259-260.
68 Hermann B. Zur Wirkung von ‘homöopathisch’ zubereitetem Thyroxin (10e-30) in Glasphiolen auf die Metamorphose vorstimulierter Rana temporaria-Larven. Graz: Interuniversitäres Kolleg.; 2005.
69 Endler P.C., Heckmann C., Lauppert E. et al. The metamorphosis of amphibian and information of thyroxin storage via the bipolar fluid water. In: Schulte J., Endler P.C. (eds). Fundamental Research in Ultra High Dilution and Homoeopathy. Dordrecht: Kluwer Academic Publishers.; 1998.
70 Endler P.C., Pongratz W., Kastberger G., Wiegant F.A.C., Haidvogel M. Climbing activity in frogs and the result of highly diluted succussed thyroxine. Br Hom J 1991; 80: 194-200.
71 Endler P.C., Pongratz W., Kastberger G., Wiegant F.A.C., Schulte J. The result of highly diluted agitated thyroxin on the climbing activity of frogs. J Vet Hum Toxicol 1994; 36: 56-59.
72 Junior R.P., Vargas L., Valentim-Zabott M., Ribeiro R.P., da Silva A.V., Otutumi L.K. Morphometry of white muscle fibers and performance of Nile tilapia (Oreochromis niloticus) fingerlings treated with methyltestosterone or a homeopathic complex. Homeopathy 2012; 101: 154-158.
73 Merlini L.S., Vargas L., Piau R., Ribeiro R.P., Merlini N.B. Results of a homeopathic complex on the performance and cortisol levels in Nile tilapia (Oreochromis niloticus). Homeopathy 2014; 103: 139-142.
74 Braccini G.L., Natali M.R., Ribeiro R.P. et al. Morpho-functional response of Nile tilapia (Oreochromis niloticus) to a homeopathic complex. Homeopathy 2013; 102: 233-241.
75 Mitra K., Kundu S.N., Khuda-Bukhsh A.R. Efficacy of a potentized homeopathic drug (Arsenicum Album-30) in reducing toxic results produced by arsenic trioxide in mice: I. On rate of accumulation of arsenic in certain organs. Complement Ther Med 1998; 6: 178-184.
76 Mitra K., Kundu S.N., Khuda-Bukhsh A.R. Efficacy of a potentized homeopathic drug (Arsenicum Album-30) in reducing toxic results produced by arsenic trioxide in mice: II. On alterations of body weight, tissue weight and total protein. Complement Ther Med 1999; 7: 24-34.
77 Datta S., Mallick P., Bukhsh A.R. Efficacy of a potentized homoeopathic drug (Arsenicum Album-30) in reducing genotoxic results produced by arsenic trioxide in mice. Complement Ther Med 1999; 7 (02) 62-75 and 7(3): 156–163.
78 Kundu S.N., Mitra K., Khuda Bukhsh A.R. Efficacy of a potentized homoeopathic drug (Arsenicum-album-30) in reducing cytotoxic results produced by arsenic trioxide in mice. Complement Ther Med 2000; 8 (02) 76-81 and 8 (3): 157–165.
79 Mallick P., Chakrabarti Mallick J., Guha B., Khuda-Bukhsh A.R. Ameliorating result of microdoses of a potentized homeopathic drug, arsenicum album, on arsenic-induced toxicity in mice. BMC Complement Alternat Med 2003; 3: 7.
80 Banerjee P., Biswas S.J., Belon P., Khuda-Bukhsh A.R. A potentized homeopathic drug, Arsenicum Album 200, can ameliorate genotoxicity induced by repeated injections of arsenic trioxide in mice. J Vet Med A Physiol Pathol Clin Med Sep 2007; 54 (07) 370-376.
81 Banerjee P., Bhattacharyya S.S., Pathak S., Naoual B., Belon P., Khuda-Bukhsh A.R. Comparative efficacy of two microdoses of a potentized homeopathic drug, arsenicum album, to ameliorate toxicity included by repeated sublethal injections of arsenic trioxide in mice. Pathobiology 2008; 75: 156-170.
82 Banerjee P., Bhattacharyya S.S., Pathak S., Boujedaini N., Belon P., Khuda-Bukhsh A.R. Evidences of protective potentials of microdoses of ultra-high diluted arsenic trioxide in mice receiving repeated injections of arsenic trioxide. Evid Based Complement Alternat Med 2009; [in press].
83 Larue F., Cal J.C., Tetau M., Buisard A.M., Guillemain J., Cambar J. Mise en evidence de l’effet Protecteur de differentes dilutions de mercurius corrosivus. Cah Biothér 1985; 88: 71-74.
84 Cal J.C., Larue F., Guillemain J., Cambar J. Chronobiological approach of protective result of mercurius corrosivus against mercury-induced nephrotoxicity. Annu Rev Chronopharmacol 1986; 3: 99-103.
85 Larue F., Cal J.C., Guillemain J., Cambar J. Influence de la durée de prétraitement sur l’effet de mercurius corrosivus vis-a-vis de la toxicité induite par le chlorure mercurique chez la souris. Homéopathie Française 1986; 74 (05) 275-281.
86 Larue F., Cal J.C., Guillemain J., Cambar J. Variations saisonnières et circadiennes de l’efficacité du prétraitement par mercurius corrosivur 15CH vis-à-vis de la toxicité induite par le chlorure mercurique. Bull du Groupe d’Etude des Rhythm Biol 1986; 18 1–2 8-9.
87 Bildet J. Etude de l’action de différentes dilutions homéopathiques de phosphore blanc (Phosphorus) sur l’hépatite toxique du rat. Thèse Université de Bordeaux II.; 1975.
88 Andresen M. Zytosolische und mitochondriale Effekte einer Intoxikation mit CCl4 am Beispiel des Lebergewebes der Ratte. Einfluss von Phosphorus D6 und Phosphorus D30. Thesis Tierärztliche Hochschule Hannover.; 1985.
89 Fisher P. The treatment of experimental lead intoxication in rats by penicillinamine and plumbum met. Proceedings of the 35th Congress of the Liga Medicorum Homeopathica Internationalis, Brighton 1982; 320-332.
90 Fisher P., House I., Belon P., Turner P. The influence of the homeopathic remedy Plumbum metallicum on the excretion kinetics of lead in rats. Hum Toxicol 1987; 6: 321-324.
91 Doutremepuich C., Aguejouf O., Pintigny D., Sertillanges M.N., De Seze O. Thrombogenic properties of ulta-low-dose of acetylsalicylic acid in a vessel model of laser-induced thrombus formation. Thromb Res 1994; 76: 225-229.
92 Belougne-Malfatti E., Aguejouf O., Doutremepuich F., Belon P., Doutrempuich C. Combination of two doses of acetyl salcylic acid: experimental study of arterial thrombosis. Thromb Res 1998; 90: 215-221.
93 Aguejouf O., Malfatti E., Belon P., Doutremepuich C. Time related neutralization of two doses acetyl salicylic acid. Thromb Res 2000; 100 (04) 317-323.
94 Eizayaga F.X., Aguejouf O., Belon P., Doutremepuich C. Platelet aggregation in portal hypertension and its modification by ultra-low doses of aspirin. Pathophysiol Haemost Thromb 2005; 34 (01) 29-34.
95 Doutremepuich C., Aguejouf O., Eizayaga F.X., Desplat V. Reverse result of aspirin: is the prothrombotic result after aspirin discontinuation mediated by cyclooxygenase 2 inhibition?. Pathophysiol Haemost Thromb 2007; 36 (01) 40-44.
96 Magnani P., Conforti A., Zanolin E., Marzotto M., Bellavite P. Dose-result study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice. Psychopharmacol Berl 2010; Jul 210 (04) 533-545.
97 Bellavite P., Magnani P., Zanolin E., Conforti A. Homeopathic doses of Gelsemium sempervirens improve the behavior of mice in response to novel environments. Evid Based Complement Alternat Med 2011; 2011: 362517.
98 Zausner-Lukitsch C. Auswirkungen von homöopathisch zubereitetem Thyroxin auf die Metamorphosegeschwindigkeit von Rana temporaria unter besonderer Berücksichtigung der Einzelhaltung und unterschiedlicher Methoden der Applikation. Thesis Vienna University.; 2001.
99 Endler P.C., Schulte J. (eds). Ultra High Dilution: Physiology and Physics. Dordrecht: Kluwer Academic Publishers.; 1994.
100 Dieterle D. Überprüfung einer Hypothese zum indirekten Einfluß potenzierter Thyroxinlösungen auf die Metamorphosegeschwindigkeit von Rana temporaria . Thesis University of Tubingen.; 1999.
101 Bellavite P., Conforti A., Marzotto M. et al. Testing homeopathy in mouse emotional response models: pooled data analysis of two series of studies. Evid Based Complement Alternat Med 2012; 2012: 954374.
102 Endler P.C., Thieves K., Reich C. et al. Replications of fundamental research models for homeopathically prepared dilutions beyond 10e-23: a bibliometric study. Homeopathy 2010; 99: 25-36.
103 Forschung im Dienste der Gesundheit (Projektträgerschaft). Unkonventionelle Medizinische Richtungen. Bestandsaufnahme zur Forschungssituation. Bonn: Wirtschaftsverlag, Verlag für neue Wissenschaft.; 1992.
104 Linde K., Jonas W.B., Melchart D., Worku F., Wagner H., Eitel F. Critical review and meta-analysis of serial agitated dilutions in experimental toxicology. Hum Exp Toxicol 1994; 13: 481-482.
105 Endler P.C. Homeopathy research – an expedition report. Edition Interuniversity College, Graz 2003/Expedition Homöopathieforschung – Ein altes Heilsystem wird plausibel Vienna: Maudrich Verlag.; 1998.
106 Endler P.C. Fundamental research into high dilution results. A classification of non-clinical research topics. In: Schulte J., Endler P.C. (eds). Fundamental Research in Ultra High Dilution and Homoeopathy. Dordrecht: Kluwer Academic Publishers.; 1998.
107 Vickers A.J. External replication of pre-clinical research in homoeopathy: a systematic review. Res Complement Med – Forsch Komplementärmed 1999; 6: 311-320.
108 Albrecht H., van Wijk R., Dittloff S. A new database on basic research in homeopathy. Homeopathy 2002; 91: 162-165.
109 Baumgartner S. Reproductions and reproducibility in homeopathy: dogma or tool?. J Altern Complement Med 2005; 11 (05) 771-772.
110 Walach H., Jones W.B., Ives J., Wijk R.V., Weingartner O. Research on homeopathy: state of the art. J Altern Complement Med 2005; 11: 813-829.
111 Göldner C. Review und Evaluierung von neuen, mit homöopathischen Zubereitungen durchgeführten toxikologischen Experimenten. Thesis University of Graz.; 2006.
112 Witt C.M., Bluth M., Albrecht H., Weisshuhn T.E., Baumgartner S., Willich S.N. The in vitro evidence for an result of high homeopathic potencies-a system review of the literature. Complement Ther Med 2007; 15: 128-138.
113 Baumgartner S. The state of basic research on homeopathy. In: Albrecht H., Witt C. (eds). New Directions in Homeopathy Research – Advice from an Interdisciplinary Conference. 2009. Essen: KVC-Verlag; 107-130.
114 Stock-Schröer B., Albrecht H., Betti L. et al. Reporting experiments in homeopathic basic research (REHBaR) – description of the checklist development. eCAM 2009; http://dx.doi.org/10.1093/ecam/nep170.
115 Stock-Schröer B., Albrecht H., Betti L. et al. Reporting experiments in homeopathic basic research (REHBaR) – a guideline for authors. Homeopathy 2009; 98: 287-298.
116 Bell I.R., Baldwin C.M., Schwartz G.E. Translating a nonlinear systems theory model for homeopathy into empirical tests. Altern Ther Health Med 2002; 8 (03) 58-66.
117 Bellavite P. Complexity science and homeopathy. A synthetic overview. Homeopathy 2003; 92: 203-212.
118 Mannaioni P.F., Mastroianni R., Mastrangelo D. Adrenaline inhibits the immunological activation of human basophils at pharmacological and ultra-low doses. Med Sci Monit 2010; 16: BR227-BR232.
119 Bellavite P., Signorini A., Marzotto M., Moratti E., Bonafini C., Olioso D. Cell sensitivity, non-linearity and inverse effects. Homeopathy 2015; 104: 139-160.
120 Bellavite P., Magnani P., Marzotto M., Conforti A. Assays of homeopathic remedies in rodent behavioural and psychopathological models. Homeopathy 2009; 98: 208-227.
121 Venard C., Boujedaini N., Mensah-Nyagan A.G., Patte-Mensah C. Comparative analysis of gelsemine and Gelsemium sempervirens activity on neurosteroid allopregnanolone formation in the spinal cord and limbic system. Evid Based Complement Alternat Med 2011; 2011: 407617.
122 Meyer L., Boujedaini N., Patte-Mensah C., Mensah-Nyagan A.G. Pharmacological effect of gelsemine on anxiety-like behavior in rat. Behav Brain Res 2013; 253: 90-94.
123 Olioso D., Marzotto M., Moratti E., Brizzi M., Bellavite P. Effects of Gelsemium sempervirens L. on pathway-focused gene expression profiling in neuronal cells. J Ethnopharmacol 2014; 153: 535-539.
124 Marzotto M., Olioso D., Brizzi M., Tononi P., Cristofoletti M., Bellavite P. Extreme sensitivity of gene expression in human SH-SY5Y neurocytes to ultra-low doses of Gelsemium sempervirens . BMC Complement Alternat Med 2014; 14: 104.
125 Bonamin L.V., Bellavite P. Immunological models in high dilution research following M Bastide. Homeopathy 2015; 104: 263-268.