Regulation der Thrombinaktivität an der Interphase zwischen Blut und Gefäßwand Interaktion der Komponenten Protein C, Protein S und Thrombomodulin

G. Müller-Berghaus; K. T. Preissner

doi:10.1055/s-0038-1660367

Hämostaseologie, Inhaltsverzeichnis

Hamostaseologie 1987; 07(01): 14-23
DOI: 10.1055/s-0038-1660367

Originalarbeit

Schattauer GmbH

Regulation der Thrombinaktivität an der Interphase zwischen Blut und Gefäßwand Interaktion der Komponenten Protein C, Protein S und Thrombomodulin

Authors

G. Müller-Berghaus

¹Klinische Forschungsgruppe für Blutgerinnung und Thrombose der Max-Planck-Gesellschaft an der Justus-Liebig-Universität, Gießen
K. T. Preissner

¹Klinische Forschungsgruppe für Blutgerinnung und Thrombose der Max-Planck-Gesellschaft an der Justus-Liebig-Universität, Gießen

Abstract

Volltext

Referenzen

LITERATUR
1 Annamalai A E, Stewart G J, Memoli M, Chio H-C, Manuel D W, Doshi K, Colman R W. Expression of factor V on human umbilical vein endothelial cells is modulated by cell injury. Arteriosclerosis 1986; 06: 196-202.
2 Awbrey B J, Hoak J C, Owen W G. Binding of human thrombin to cultured human endothelial cells. J Biol Chem 1979; 254: 4092-5.
3 Barrett A J. α₂-Macroglobulin. Meth Enzymol 1981; 80: 737-54.
4 Bauer P I, Machovich R, Arânyl P, Kalman G B, Csonka E, Horvath I. Mechanism of thrombin binding to endothelial cells. Blood 1983; 61: 368-72.
5 Bertina R M, Cupers R, Van Wijngaarden A. Factor IXa protects activated factor VIII against inactivation by activated protein C. Biochem Biophys Res Commun 1984; 125: 177-83.
6 Blackburn M N, Sibley C C. The heparin binding site of antithrombin III. J Biol Chem 1980; 255: 824-6.
7 Broekmans A W, Bertina R M. Protein C. In: Poller L. (ed) Recent Advances in Blood Coagulation. Edinburgh: Churchill Livingstone; 1985: 117-37.
8 Brox J H, Østerud B, BjØrklid E, Fenton J W. Production and availability of thromboplastin in endothelial cells: The effects of thrombin, endotoxin and platelets. Br J Haematol 1984; 57: 239-46.
9 Buonassisi V. Sulfated mucopolysaccharide synthesis and secretion in endothelial cell cultures. Exp Cell Res 1973; 76: 363-8.
10 Busch C, Owen W G. Identification in vitro of an endothelial cell surface cofactor for antithrombin III. J Clin Invest 1982; 69: 726-9.
11 Cerveny T J, Fass D N, Mann K G. Synthesis of coagulation factor V by cultured aortic endothelium. Blood 1984; 63: 1467-74.
12 Clouse L H, Comp P C. The regulation of hemostasis: the protein C system. N Engl J Med 1986; 314: 1298-1304.
13 Colburn P, Buonassisi V. Anti-clotting activity of endothelial cell cultures and heparin sulfate proteoglycans. Biochem Biophys Res Commun 1982; 104: 220-7.
14 Coldwell PRB, Seegal B C, Hsu K C. Angiotensin-converting enzyme: vascular endothelial localization. Science 1976; 191: 1050-1.
15 Colucci M, Balconi G, Lorenzet R, Pietra A, Locati D, Donati M B, Semararo N. Cultured human endothelial cells generate tissue factor in response to endotoxin. J Clin Invest 1983; 71: 1893-6.
16 Colucci M, Stassen J M, Collen D. Influence of protein C activation on blood coagulation and fibrinolysis in squirrel monkeys. J Biol Chem 1984; 74: 200-4.
17 Comp P C. Animal studies of protein C physiology. Sem Thromb Hemostas 1984; 10: 149-3.
18 Comp P C, Esmon C T. Generation of fibrinolytic activity by infusion of activated protein C into dogs. J Clin Invest 1981; 68: 1221-8.
19 Conard J, Horellou M H, Teger-Nilsson A C, De Carbonnières C, Samama M. The fibrinolytic system in 15 patients with congenital protein C deficiency. (Abstract). Haemostasis 1984; 14: 25.
20 Dahlbäck B. Purification of human vitamin K-dependent protein S and its limited proteolysis by thrombin. Biochem J 1983; 209: 837-46.
21 Dahlbäck B. Purification of human C4b-binding protein and formation of its complex with vitamin K-dependent protein S. Biochem J 1983; 209: 847-56.
22 Dahlbäck B. Interaction between vitamin K-dependent protein S and the complement protein, C4b-binding protein. A link between coagulation and the complement system. Sem Thromb Hemostas 1984; 10: 139-48.
23 Dahlbäck B, Stenflo J. Inhibitory effect of activated protein C on activation of prothrombin by platelet-bound factor Xa. Eur J Biochem 1980; 107: 331-5.
24 Dahlbäck B, Stenflo J. High molecular weight complex in human plasma between vitamin K-dependent protein S and complement component C4b-binding protein. Proc Natl Acad Sci USA 1981; 78: 2512-6.
25 DeBault L E, Esmon N L, Olson J R, Esmon C T. Distribution of the thrombomodulin antigen in rabbit vasculature. Lab Invest 1986; 54: 172-8.
26 DeBault L E, Esmon N L, Smith G P, Esmon C T. Localization of thrombomodulin antigen in rabbit endothelial cells in culture. Lab Invest 1986; 54: 179-87.
27 Delvos U, Meusel P, Preissner K T, Müller-Berghaus G. Formation of activated protein C and inactivation of cell-bound thrombin by antithrombin III at the surface of cultured vascular endothelial cells. A comparative study of two anticoagulant mechanisms. (Eingereicht zur Veröffentlichung).
28 Delvos U, Meusel P, Preissner K T, Müller-Berghaus G. Modulation of protein C activation by thrombin/antithrombin III interactions at the endothelial cell surface. Blood 1985; 66: 355A
29 DiScipio R G, Davie E W. Characterization of protein S, a γ-carboxyglutamic acid containing protein from bovine and human plasma. Biochemistry 1979; 18: 899-904.
30 Downing M R, Bloom J W, Mann K G. Comparison of the inhibition of thrombin by three plasma protease inhibitors. Biochemistry 1978; 17: 2649-53.
31 Drakenberg T, Fernlund P, Roepstorff P, Stenflo J. ß-Hydroxyaspartic acid in vitamin K-dependent protein C. Proc Natl Acad Sci USA 1983; 80: 1802-6.
32 Esmon C T. Protein C: biochemistry, physiology, and clinical implications. Blood 1983; 62: 1155-8.
33 Esmon C T, Owen W G. Identification of an endothelial cell cofactor for thrombin catalyzed activation of protein C. Proc Natl Acad Sci USA 1981; 78: 2249-52.
34 Esmon N L, Carroll R C, Esmon C T. Thrombomodulin blocks the ability of thrombin to activate platelets. J Biol Chem 1983; 258: 12238-42.
35 Esmon C T, Esmon N L, Harris K W. Complex formation between thrombin and thrombomodulin inhibits both thrombincatalyzed fibrin formation and factor V activation. J Biol Chem 1982; 257: 7944-7.
36 Esmon N L, Owen W G, Esmon C T. Isolation of a membrane-bound cofactor for thrombin-catalyzed activation of protein C. J Biol Chem 1982; 257: 859-64.
37 Fair D S, Marlar R A. Biosynthesis and secretion of factor VII, protein C, protein S, and the protein C inhibitor from a human hepatoma cell line. Blood 1986; 67: 64-70.
38 Fair D S, Marlar R A, Levin E G. Human endothelial cells synthesize protein S. Blood 1986; 67: 1168-71.
39 Fernlund P, Stenflo J. ß-Hydroxyaspartic acid in vitamin K-dependent proteins. J Biol Chem 1983; 258: 125509-12.
40 Foster D, Davie E W. Characterization of cDNA coding for human protein C. Proc Natl Acad Sci USA 1984; 81: 4766-70.
41 de Fouw N J, Haverkate F, Bertina R M, Koopman J, van Wijngaarden A, van Hinsbergh V W M. The cofactor role of protein S in the acceleration of whole blood clot lysis by activated protein C in vitro. Blood 1986; 67: 1189-92.
42 Fulcher C A, Gardiner J E, Griffin J H, Zimmerman T S. Proteolytic inactivation of human factor VIII procoagulant protein by activated human protein C and its analogy with factor V. Blood 1984; 63: 486-9.
43 Gaidai K S, Evensen S A, Brosstad F. Effects of thrombin on the integrity of monolayers of cultured human endothelial cells. Thromb Res 1982; 27: 575-84.
44 Gallagher J T, Lyon M, Steward W P. Structure and function of heparan sulphate proteoglycans. Biochem J 1986; 236: 313-25.
45 Ganguly P, Gould N L. Thrombin receptors on human platelets : thrombin binding and antithrombin properties of glycoprotein I. Br J Haematol 1979; 42: 137-45.
46 Gelehrter T D, Sznycer-Laszuk R. Thrombin induction of plasminogen activator-inhibitor in cultured human endothelial cells. J Clin Invest 1986; 77: 165-9.
47 Griffin J H, Evatt B, Zimmerman T S, Kleiss A J, Wideman C. Deficiency of protein C in congenital thrombotic disease. J Clin Invest 1981; 68: 1370-3.
48 Guinto E R, Esmon C T. Loss of prothrombin and of factor Xa - factor Va interactions upon inactivation of factor V by activated protein C. J Biol Chem 1984; 259: 13986-92.
49 Harpel P C. Blood proteolytic enzyme inhibitors: Their role in modulating blood coagulation and fibrinolytic enzyme pathways. In: Colman R W, Hirsh J, Marder V J, Salzman E W. (eds). Hemostasis and Thrombosis, Basic Principles and Clinical Practice. Philadelphia: Lippincott; 1982: 738-47.
50 Hatton M W C, Dejana E, Cazenave J P, Regoeczi E, Mustard J F. Heparin inhibits thrombin binding to rabbit thoracic aorta endothelium. J Lab Clin Med 1980; 96: 861-70.
51 Heimburger N. Proteinase inhibitors of human plasma. Their properties and control functions. In: Reich E, Rifkin D B, Shaw E. (eds). Proteases and Biological Control. Cold Spring Harbor Conferences on Cell Proliferation. 1975: 367-86.
52 Heimburger N, Haupt H, Kranz T, Baudner S. Humanserumproteine mit hoher Affinität zu Carboxymethylcellulose, II. Hoppe Seyler’s Z Physiol Chem 1972; 353: 1133-40.
53 van Hinsbergh V W M, Bertina R M, van Wijngaarden A, van Tilburg N H, Emeis J J, Haverkate F. Activated protein C decreases plasminogen activator-inhibitor activity endothelial cell-conditioned medium. Blood 1985; 65: 444-51.
54 Hofsteenge J, Taguchi H, Stone S R. Effect of thrombomodulin on the kinetics of the interaction of thrombin with substrates and inhibitors. Biochem J 1986; 237: 243-51.
55 Isaacs J D, Savion N, Gospodarowicz D, Fenton J W, Shuman M A. Covalent binding of thrombin to specific sites on corneal endothelial cells. Biochemistry 1981; 20: 398-403.
56 Ishii H, Majerus P W. Thrombomodulin is present in human plasma and urine. J Clin Invest 1985; 76: 2178-81.
57 Ishii H, Salem H H, Bell C E, Laposata E A, Majerus P W. Thrombomodulin, an endothelial anticoagulant protein, is absent from the human brain. Blood 1986; 67: 362-5.
58 Jaffe E A, Nachman R L, Becker C G, Minick C R. Culture of human endothelial cells derived from umbilical cord veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973; 52: 2745-56.
59 Jakubowski H V, Kline M D, Owen W G. The effect of bovine thrombomodulin on the specificity of bovine thrombin. J Biol Chem 1986; 261: 3876-82.
60 Jordan R E, Favreau L V, Braswell E H, Rosenberg R D. Heparin with two binding sites for antithrombin or platelet factor 4. J Biol Chem 1982; 257: 400-6.
61 Kisiel W. Human plasma protein C. Isolation, characterization, and mechanism of activation by α-thrombin. J Clin Invest 1979; 64: 761-9.
62 Kisiel W, Canfield W M, Ericsson L H, Davie E W. Anticoagulant properties of bovine plasma protein C following activation by thrombin. Biochemistry 1977; 16: 5824-31.
63 Kisiel W, Ericsson L H, Davie E W. Proteolytic activation of protein C from bovine plasma. Biochemistry 1976; 15: 4893-900.
64 Kurosawa S, Aoki N. Preparation of thrombomodulin from human placenta. Thromb Res 1985; 37: 353-64.
65 Levin E G, Marzek U, Anderson J, Harker L A. Thrombin stimulates tissue plasminogen activator release from cultured human endothelial cells. J Clin Invest 1984; 74: 1988-95.
66 Levine J D, Harlan J M, Harker L A, Joseph M L, Counts R B. Thrombinmediated release of factor VIII antigen from human umbilical vein endothelial cells in culture. Blood 1982; 60: 531-4.
67 Lieberman G E, Lewis G P, Peters T J. A membrane-bound enzyme in rabbit aorta capable of inhibiting adenosine-diphosphate induced platelet aggregation. Lancet 1977; II: 330-2.
68 Lijnen H R, Hoylaerts M, Collen D. Heparin binding properties of human histidine-rich glycoprotein. Mechanism and role in the neutralization of heparin in plasma. J Biol Chem 1983; 258: 3803-8.
69 Liu C Y, Nossel H L, Kaplan K L. The binding of thrombin by fibrin. J Biol Chem 1979; 254: 10421-5.
70 Lockhart M S, Taylor Jr F B, Vigano SD'Angelo, D’Angelo A. Neutralization of plasminogen activator inhibitor by activated protein C: The role of protein S and phospholipids. (Abstract). Fibrinolysis 1986; Suppl1: 38.
71 Lollar P, Owen W G. Clearance of thrombin from circulation in rabbit by highaffinity binding sites on endothelium. J Clin Invest 1980; 66: 1222-30.
72 Lollar P, Hoak J C, Owen W G. Binding of thrombin to cultured human endothelial cells. J Biol Chem 1980; 255: 10279-83.
73 Lollar P, Macintosh S C, Owen W G. Interaction of thrombin with the vascular endothelium in a recirculating Langendorff rabbit heart preparation. Ann NY Acad Sci 1982; 401: 234-40.
74 Lollar P, Macintosh S C, Owen W G. Reaction of antithrombin III with thrombin bound to the vascular endothelium. J Biol Chem 1984; 259: 4335-8.
75 Long G L, Belagaje R M, MacGillivray R T A. Cloning and sequencing of liver cDNA coding for bovine protein C. Proc Natl Acad. Sci USA 1984; 81: 5653-6.
76 Longas M O, Finlay T H. The covalent nature of the human antithrombin III-thrombin bond. Biochem J 1980; 189: 481-9.
77 Polgar J, Lérânt I, Muszbek L, Machouich R. Thrombomodulin inhibits the activation of factor XIII by thrombin. Thromb Res 1986; 43: 585-90.
78 Mammen E F. Protein C und S. Hämostaseol 1984; 04: 138-47.
79 Mammen E F, Thomas W R, Seegers W H. Activation of purified prothrombin to autoprothrombin I or autoprothrombin II (platelet cofactor II) or autoprothrombin II-A. Thromb Diath Haemorrh 1960; 05: 218-50.
80 Marciniak E. Coagulation inhibitor elicited by thrombin. Science 1970; 170: 452-3.
81 Marcum J A, Rosenberg R D. Anticoagulantly active heparin-like molecules from vascular tissue. Biochemistry 1984; 23: 1730-7.
82 Marcum J A, Rosenberg R D. Heparinlike molecules with anticoagulant activity are synthesized by cultured endothelial cells. Biochem Biophys Res Com 1985; 126: 365-72.
83 Marcum J A, McKenney J B, Rosenberg R D. Acceleration of thrombin-antithrombin complex formation in rat hindquaters via heparin-like molecules bound to the endothelium. J Clin Invest 1984; 74: 341-50.
84 Marlar R A. Single chain protein C is enzymatically similar to the two chain form of protein C. Biochim Biophys Acta. (Im Druck).
85 Marlar R A, Kleiss A J, Griffin J H. Mechanism of action of human activated protein C, a thrombin-dependent anticoagulant enzyme. Blood 1982; 59: 1067-72.
86 Maruyama I, Majerus P W. The turnover of thrombin-thrombomodulin complex in cultured human umbilical vein endothelial cells and A549 lung cancer cells. J Biol Chem 1985; 260: 15432-8.
87 Maruyama I, Bell C E, Majerus P W. Thrombomodulin is found on endothelium of arteries, veins, capillaries, and lymphatics, and on syncytiotrophoblast of human placenta. J Cell Biol 1985; 101: 363-71.
88 Maruyama I, Salem H H, Majerus P W. Coagulation factor Va binds to human umbilical vein endothelial cells and accelerates protein C activation. J Clin Invest 1984; 74: 224-30.
89 Messmore Jr H L. Natural inhibitors of the coagulation system. Sem Thromb Hemostas 1982; 08: 267-75.
90 Meusel P, Preissner K T, Delvos U, Müller-Berghaus G. Aktivierung von Protein C an der Oberfläche kultivierter Endothelzellen: Die Rolle des Antithrombin III. Verh Deutsch Ges Inn Med. 1986 (Im Druck).
91 Moncada S, Vane J R. Arachidonic acid metabolites and the interactions between platelets and blood-vessel walls. N Engl J Med 1979; 300: 1142-7.
92 Müller-Berghaus G, Lasch H-G. Microcirculatory Disturbances Induced by Generalized Intravascular Coagulation. Handbuch exp Pharmakol 1975; XVI/3: 429-514.
93 Owen W G, Esmon C T. Functional properties of an endothelial cell cofactor for thrombin-catalyzed activation of protein C. J Biol Chem 1981; 256: 5532-5.
94 Owen W G, Brennan S O, Lewis J H, Carrell R W. Mutation of antitrypsin to antithrombin. N Engl J Med 1983; 309: 694-8.
95 Owen W G, Penick G D, Yoder E, Poole B L. Evidence for an esterbond between thrombin and heparin cofactor. Thromb Haemostas 1976; 35: 87-95.
96 Pearson J D, Gordon J L. Vascular endothelial and smooth muscle cells in culture selectively release adenine nucleotides. Nature 1979; 281: 384-6.
97 Preissner K T, Müller-Berghaus G. Molekulare Wechselwirkungen zwischen Komplement-, Gerinnungs- und Fibrinolysesystem. Hämostaseol 1986; 06: 67-81.
98 Preissner K T, Müller-Berghaus G. S protein modulates the heparin-catalyzed inhibition of thrombin by antithrombin III. Evidence for a direct interaction of S protein with heparin. Eur J Biochem 1986; 156: 645-50.
99 Preissner K T, Delvos U, Müller-Berghaus G. Binding of thrombin to thrombomodulin accelerates the inhibition of the enyzme by antithrombin III. Evidence for a heparin-independent mechanism. (Eingereicht zur Veröffentlichung).
100 Preissner K T, Wassmuth R, Müller-Berghaus G. Physicochemical characterization of human S-protein and its function in blood coagulation. Biochem J 1985; 231: 349-55.
101 Prescott S M, Zimmerman G A, McIntyre T M. Human endothelial cells in culture produce platelet-activating factor (1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) when stimulated with thrombin. Proc Natl Acad Sci USA 1984; 81: 3534-8.
102 Rosenberg R D. Heparin, antithrombin, and abnormal clotting. Ann Rev Med 1978; 29: 367-78.
103 Rosenberg R D. Role of heparin and heparinlike molecules in thrombosis and atherosclerosis. Fed Proc 44 1985; 404-9.
104 Rosenberg R D, Damus P S. The purification and mechanism of action of human antithrombin-heparin cofactor. J Biol Chem 1973; 248: 6490-5.
105 Sakata Y, Curriden S, Lawrence D, Griffin J H, Loskutoff D J. Activated protein C stimulates the fibrinolytic activity of cultured endothelial cells and decreases antiactivator activity. Proc Natl Acad Sci USA 1985; 82: 1121-5.
106 Salem H H, Broze G J, Miletich J P, Majerus P W. The light chain of factor Va contains the activity of factor Va that accelerates protein C activation by thrombin. J Biol Chem 1983; 258: 8531-4.
107 Salem H H, Broze G J, Miletich J P, Majerus P W. Human coagulation factor Va is a cofactor for the activation of protein C. Proc Natl Acad Sci USA 1983; 80: 1584-8.
108 Salem H H, Esmon N L, Esmon C T, Majerus P W. Effects of thrombomodulin and coagulation factor Va-light chain on protein C activation in vitro. J Clin Invest 1984; 73: 968-72.
109 Salem H H, Maruyama I, Ishii H, Majerus P W. Isolation and characterization of thrombomodulin from human placenta. J Biol Chem 1984; 259: 12246-51.
110 Scully M F, Kakkar V V. Identification of heparin cofactor II as the principal plasma cofactor for the antithrombin activity of pentosan polysulphate (SP54). Thromb Res 1984; 36: 187-94.
111 Scuttie J W. Mechanism of action of vitamin K: Synthesis of gamma-carboxy glutamic acid. CRC Crit Rev Biochem 1980; 08: 191-223.
112 Seegers W H, McCoy L E, Groben H D, Sakuragawa N, Agrawal B L. Purification and some properties of autoprothrombin II-A: An anticoagulant perhaps also related to fibrinogen. Thromb Res 1972; 01: 443-60.
113 Shimada K, Ozawa T. Evidence that cell surface heparan sulfate is involved in the high affinity thrombin binding to cultured porcine aortic endothelial cells. J Clin Invest 1985; 75: 1308-16.
114 Stenflo J. A new vitamin K-dependent protein. J Biol Chem 1976; 251: 355-63.
115 Stenflo J. Vitamin K, prothrombin, and γ-carboxyglutamic acid. Adv Enzymol 1981; 46: 1-31.
116 Stenflo J. Structure and function of protein C. Sem Thromb Hemostas 1984; 10: 109-21.
117 Stenflo J, Fernlund P. Amino acid sequence of the heavy chain of bovine protein C. J Biol Chem 1982; 257: 12180-90.
118 Stern D, Brett J, Harris K, Nawroth P. Participation of endothelial cells in the protein C-protein S anticoagulant pathway: the synthesis and release of protein S. J Cell biol 1986; 102: 1971-8.
119 Stern D, Nawroth P, Marcum J, Handley D, Kisiel W, Rosenberg R, Stern K. Interaction of antithrombin III with bovine aortic segments. J Clin Invest 1985; 75: 272-9.
120 Stocker K, Fischer H, Meier J, Brogli N, Svendson N. Protein C activator in snake venoms. Behring Inst Mitt 1986; 79: 37-47.
121 Suzuki K. Activated protein C inhibitor. Sem Thromb Hemostas 1984; 10: 154-61.
122 Suzuki K, Nishioka J, Hashimoto S. Protein C inhibitor. Purification from human plasma and characterization. J Biol Chem 1983; 258: 163-8.
123 Suzuki K, Nishioka J, Kusumoto H, Hashimoto S. Mechanism of inhibition of activated protein C by protein C inhibitor. J Biochem 1984; 95: 187-95.
124 Suzuki K, Stenflo J, Dahlbäck B, Teodorsson B. Inactivation of human coagulation factor V by activated protein C. J Biol Chem 1983; 258: 1914-20.
125 Thompson E A, Salem H H. Inhibition by human thrombomodulin of factor Xa-mediated cleavage of prothrombin. J Clin Invest 1986; 78: 13-7.
126 Tollefsen D M, Blank M K. Detection of a new heparin-dependent inhibitor of thrombin in human plasma. J Clin Invest 1981; 68: 589-96.
127 Tollefsen D M, Pestka C A. Modulation of heparin cofactor II activity by histidinerich glycoprotein and platelet factor 4. J Clin Invest 1985; 75: 496-501.
128 Tollefsen D M, Majerus D W, Blank M K. Heparin cofactor II. Purification and properties of a heparin-dependent inhibitor of thrombin in human plasma. J Biol Chem 1982; 257: 2162-9.
129 Tollefsen D M, Pestka C A, Monafo W J. Activation of heparin cofactor II by dermatan sulfate. J Biol Chem 1983; 258: 6713-6.
130 Van de Waart P, Bruis H, Hemker H C, Lindhout T. Functional properties of factor Va subunits after proteolytic alterations by activated protein C. Biochim Biophys Acta 1984; 799: 38-44.
131 Walker J F. Regulation of bovine activated protein C by protein S: The role of the cofactor protein in species specificity. Thromb Res 1981; 22: 321-7.
132 Walker F J. Regulation of activated protein C by protein S. The role of phospholipid in factor Va inactivation. J Biol Chem 1981; 256: 11128-31.
133 Walker F J. Protein S and the regulation of activated protein S. Sem Thromb Hemostas 1984; 10: 131-8.
134 Walker J F, Sexton P W, Esmon C T. The inhibition of blood coagulation by activated protein C through the selective inactivation of activated factor V. Biochim Biophys Acta 1979; 571: 333-42.
135 Wang D, Yuan A I, Feinman R D. Structure of a₂-macroglobulin-protease complexes. Ann NY Acad Sci 1983; 421: 90-97.
136 Weimar B, Delvos U. The mechanism of fibrin-induced disorganization of cultured human endothelial cell monolayers. Arteriosclerosis 1986; 06: 139-45.
137 Weksler B B, Marcus A J, Jaffe E A. Synthesis of prostaglandin I₂ (prostacyclin) by cultured human and bovine endothelial cells. Proc Natl Acad Sci USA 1977; 74: 3922-6.
138 Wolinsky M. A proposal linking clearance of circulating lipoproteins to tissue metabolic activity as a basis for understanding atherogenesis. Circ Res 1980; 47: 301-11.