Ultra High Dilution of triiodothyronine modifies cellular apoptosis in Rana catesbeiana tadpole tail in vitro

J.R.P. Guedes; S. Carrasco; C.M. Ferreira; L.V. Bonamin; W. Souza; C. Goldenstein-Schainberg; E.R. Parra; V.L. Capelozzi

doi:10.1016/j.homp.2011.05.007

Homeopathy, Table of Contents

Homeopathy 2011; 100(04): 220-227
DOI: 10.1016/j.homp.2011.05.007

Original Paper

Ultra High Dilution of triiodothyronine modifies cellular apoptosis in Rana catesbeiana tadpole tail in vitro

J.R.P. Guedes

¹Laboratory of Molecular Pathology, Department of Pathology, University of São Paulo School of Medicine, SP, Brazil

,

S. Carrasco

²Discipline of Rheumatology, School of Medicine, Clinics Hospital, University of São Paulo, Brazil

,

C.M. Ferreira

³Agricultural Department of São Paulo State, Aquaculture Research Center, Fishery Institute, Brazil

,

L.V. Bonamin

⁴Research Center, University Paulista, Brazil

⁵Laboratory of Veterinary Pathology, University of Santo Amaro, Brazil

,

W. Souza

¹Laboratory of Molecular Pathology, Department of Pathology, University of São Paulo School of Medicine, SP, Brazil

,

C. Goldenstein-Schainberg

²Discipline of Rheumatology, School of Medicine, Clinics Hospital, University of São Paulo, Brazil

,

E.R. Parra

¹Laboratory of Molecular Pathology, Department of Pathology, University of São Paulo School of Medicine, SP, Brazil

,

V.L. Capelozzi

¹Laboratory of Molecular Pathology, Department of Pathology, University of São Paulo School of Medicine, SP, Brazil

› Author Affiliations
Subject Editor:

Abstract

Background: Ultra High Dilutions (UHD) are diluted beyond the Avogadro limit with dynamization (dilution with succussion). The process of anuran amphibian metamorphosis is controlled by thyroid hormones, including the resorption of the tadpole tail.

Methods: A randomized and blinded study was performed to investigate the influence of triiodothyronine (T3) 5 · 10⁻²⁴ M (10cH) on apoptosis induced by T3 100 nM in Rana catesbeiana tadpoles’ tail tips, in vitro. Explants were randomized to three groups: control: no T3 in pharmacological or UHD dose; test: T3 100 nM and challenged with T3 10cH (UHD); positive control: T3 100 nM, treated with unsuccussed ethanol. The apoptotic index and the area of explants of test and control groups at the first and final day of the experiment were compared by t-test.

Results: There was no difference in tail tip area between test and control groups, but a significantly higher (p < 0.01) index of apoptosis in explants of the test group.

Conclusion: This data suggest that T3 10cH modifies the effect of T3 at pharmacological dose, opening new perspectives for further studies and investigation of the dose–effect curve.

Keywords

Ultra High Dilution - Triiodothyronine - Apoptosis - Rana catesbeiana - Organ culture - Metamorphosis - Endo-isopathy - Homeopathy

Full Text

References

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