Lymphocyte proliferation stimulated by activated Cebus apella macrophages treated with a complex homeopathic immune response modifiers

Carlos Onete Coelho Moreira; Joana de Fátima Ferreira Borges da Costa; Mariana Ferreira Leal; Edilson Ferreira de Andrade; Alexandre Pingarilho Rezende; Aline Amaral Imbeloni; José Augusto Pereira Carneiro Muniz; Marília de Arruda Cardoso Smith; Rommel Rodríguez Burbano; Paulo Pimentel de Assumpção

doi:10.1016/j.homp.2011.09.001

Homeopathy, Inhaltsverzeichnis

Homeopathy 2012; 101(01): 74-79
DOI: 10.1016/j.homp.2011.09.001

Original Paper

Lymphocyte proliferation stimulated by activated Cebus apella macrophages treated with a complex homeopathic immune response modifiers

Carlos Onete Coelho Moreira

¹Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil

²Serviço de Cirurgia, Hospital Universitário João de Barros Barreto, Universidade Federal do Pará, Belém, PA 66073-000, Brazil

,

Joana de Fátima Ferreira Borges da Costa

¹Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil

,

Mariana Ferreira Leal

³Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, SP 04023-900, Brazil

,

Edilson Ferreira de Andrade

¹Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil

,

Alexandre Pingarilho Rezende

¹Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil

,

Aline Amaral Imbeloni

⁴Centro Nacional de Primatas, Ministério da Saúde, Ananindeua, PA 77030-000, Brazil

,

José Augusto Pereira Carneiro Muniz

⁴Centro Nacional de Primatas, Ministério da Saúde, Ananindeua, PA 77030-000, Brazil

,

Marília de Arruda Cardoso Smith

³Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo, SP 04023-900, Brazil

,

Rommel Rodríguez Burbano

¹Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil

,

Paulo Pimentel de Assumpção

²Serviço de Cirurgia, Hospital Universitário João de Barros Barreto, Universidade Federal do Pará, Belém, PA 66073-000, Brazil

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Verantwortlicher Herausgeber dieser Rubrik:

Abstract

Introduction: Canova is a complex homeopathic medicine that enhances a specific immunologic responses against several exogenous and endogenous conditions. Canova activates macrophages both in vivo and in vitro.

Aim and method: We evaluated the effects of macrophages activated by Canova in vivo and ex vitro in the proliferation of lymphocytes. Canova was used to activate Cebus apella macrophages in vivo or ex vitro with Canova. Lymphocytes were cultured with the macrophage culture medium. The analysis of Canova effects in cultured lymphocytes was performed according to the cell cycle phase using flow cytometry. The Interferon gamma and Interleukin-5 cytokines quantification in these lymphocyte culture media was performed by Enzyme-linked immunosorbent assay (ELISA).

Results: We observed that Canova actives macrophages in vivo and ex vitro. The lymphocytes cultured in a supplemented medium with macrophages activated by Canova treatment presented a higher number of proliferation cells than lymphocytes not exposed to macrophages activated by Canova. The Interferon gamma and Interleukin-5 cytokines were only observed in the medium of lymphocytes exposed to macrophages activated by Canova. Thus, Canova has potential as a new adjuvant therapy.

Keywords

Canova - Immune response modification - Cell cycle - Cytokines - Non-human primates - Cebus apella

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Referenzen

References
1 Da Rocha Piemonte M., De Freitas Buchi D. Analysis of IL-2, IFN-gamma and TNF-alpha production, alpha5 beta1 integrins and actin filaments distribution in peritoneal mouse macrophages treated with homeopathic medicament. J Submicrosc Cytol Pathol 2002; 34: 255-263.
2 Cheever M.A., Schlom J., Weiner L.M. et al. Translational Research Working Group developmental pathway for immune response modifiers. Clin Cancer Res 2008; 14: 5692-5699.
3 Varin A., Gordon S. Alternative activation of macrophages: immune function and cellular biology. Immunobiology 2009; 214: 630-641.
4 Hu X., Chakravarty S.D., Ivashkiv L.B. Regulation of interferon and Toll-like receptor signaling during macrophage activation by opposing feedforward and feedback inhibition mechanisms. Immunol Rev 2008; 226: 41-56.
5 Roitt I.B.J., Male D. Immunology. New York: Mosby; 2001.
6 Cesar B., Abud A.P., de Oliveira C.C. et al. Activation of mononuclear bone marrow cells treated in vitro with a complex homeopathic medication. Micron 2008; 39: 461-470.
7 Burbano R.R., Leal M.F., da Costa J.B. et al. Lymphocyte proliferation stimulated by activated human macrophages treated with Canova. Homeopathy 2009; 98: 45-48.
8 Pereira W.K., Lonardoni M.V., Grespan R., Caparroz-Assef S.M., Cuman R.K., Bersani-Amado C.A. Immunomodulatory effect of Canova medication on experimental Leishmania amazonensis infection. J Infect 2005; 51: 157-164.
9 de Oliveira C.C., de Oliveira S.M., Godoy L.M., Gabardo J., Buchi Dde F. Canova, a Brazilian medical formulation, alters oxidative metabolism of mice macrophages. J Infect 2006; 52: 420-432.
10 Lopes L., Godoy L.M., de Oliveira C.C., Gabardo J., Schadeck R.J., de Freitas Buchi D. Phagocytosis, endosomal/lysosomal system and other cellular aspects of macrophage activation by Canova medication. Micron 2006; 37: 277-287.
11 Abud A.P., Cesar B., Cavazzani L.F., de Oliveira C.C., Gabardo J., Buchi D.F. Activation of bone marrow cells treated with Canova in vitro. Cell Biol Int 2006; 30: 808-816.
12 Seligmann I.C., Lima P.D., Cardoso P.C. et al. The anticancer homeopathic composite “Canova Method” is not genotoxic for human lymphocytes in vitro. Genet Mol Res 2003; 2: 223-228.
13 Sharma N., Trikha P., Athar M., Raisuddin S. Protective effect of Cassia occidentalis extract on chemical-induced chromosomal aberrations in mice. Drug Chem Toxicol 1999; 22: 643-653.
14 Preston R.J., San Sebastian J.R., McFee A.F. The in vitro human lymphocyte assay for assessing the clastogenicity of chemical agents. Mutat Res 1987; 189: 175-183.
15 Nicoletti I., Migliorati G., Pagliacci M.C., Grignani F., Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 1991; 139: 271-279.
16 Garcez L.M., Goto H., Ramos P.K. et al. Leishmania (Leishmania) amazonensis-induced cutaneous leishmaniasis in the primate Cebus apella: a model for vaccine trials. Int J Parasitol 2002; 32: 1755-1764.
17 Fuchs E., Tumbar T., Guasch G. Socializing with the neighbors: stem cells and their niche. Cell 2004; 116: 769-778.
18 Li D., Wang G.Y., Liu Z.F., Shi Y.X., Zhang H., Bai Z.L. Macrophage-associated erythropoiesis and lymphocytopoiesis in mouse fetal liver: ultrastructural and ISH analysis. Cell Biol Int 2004; 28: 457-461.
19 Guimaraes F.S., Abud A.P., Oliveira S.M. et al. Stimulation of lymphocyte anti-melanoma activity by co-cultured macrophages activated by complex homeopathic medication. BMC Cancer 2009; 9: 293.
20 Sato D.Y., Wal R., de Oliveira C.C. et al. Histopathological and immunophenotyping studies on normal and sarcoma 180-bearing mice treated with a complex homeopathic medication. Homeopathy 2005; 94: 26-32.
21 Chikanza I.C., Grossman A.B. Reciprocal interactions between the neuroendocrine and immune systems during inflammation. Rheum Dis Clin North Am 2000; 26: 693-711.
22 Guthridge M.A., Stomski F.C., Thomas D. et al. Mechanism of activation of the GM-CSF, IL-3, and IL-5 family of receptors. Stem Cells 1998; 16: 301-313.
23 Stumpo R., Kauer M., Martin S., Kolb H. IL-10 induces gene expression in macrophages: partial overlap with IL-5 but not with IL-4 induced genes. Cytokine 2003; 24: 46-56.