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pferde spiegel 2019; 22(02): 75-88
DOI: 10.1055/a-0858-4508
CVE-Fortbildung
Georg Thieme Verlag KG Stuttgart · New York

Aktuelle Empfehlungen zur Diagnostik und Therapie der Endometritis bei der Stute

Jutta Sielhorst
,
Martin Köhne
,
Kim-Carolin Spilker
,
Anne Kahler
,
Christiane Brekle
,
Anna Tönissen
,
Rebekka Käser
,
Dominik Burger
,
Harald Sieme
Weitere Informationen

Publikationsverlauf

Publikationsdatum:
21. Juni 2019 (online)

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Die Endometritis stellt die Hauptursache für eine reduzierte Fruchtbarkeit der Stute dar und wird bei 25 – 60% der güsten Stuten beobachtet. Die Identifizierung der Endometritisursache, das Bedeckungs-/Besamungsmanagement und die gezielte Therapie sind essentiell für den Trächtigkeitserfolg dieser Problemstuten.
 

  • Literatur

  • 1 Christoffersen M, Troedsson M. Inflammation and fertility in the mare. Reprod Domest Anim 2017; 52 (03) 14-20 doi:10.1111/rda.13013
    CrossrefPubMedGoogle Scholar
  • 2 Woodward EM, Troedsson MH. Inflammatory mechanisms of endometritis. Equine Vet J 2015; 47 (04) 384-389 doi:10.1111/evj.12403
    CrossrefPubMedGoogle Scholar
  • 3 Riddle W, LeBlanc M, Stromberg A. Relationships between uterine culture, cytology and pregnancy rates in a Thoroughbred practice. Theriogenol 2007; 68: 395-402
    CrossrefPubMedGoogle Scholar
  • 4 Troedsson MHT, Demoraes M, Liu I. Correlations between histologic endometrial lesions in mares and clinical response to intrauterine exposure with Streptococcus zooepidemicus. Am J Vet Res 1993; 54: 570-572
    PubMedGoogle Scholar
  • 5 Woodward EM, Christoffersen M, Campos J, Squires W, Troedsson MHT. Susceptibility to persistent breeding-induced endometritis in the mare: relationship to endometrial biopsy score and age, and variations between seasons. Theriogenol 1978; 495-501
    Google Scholar
  • 6 Quetin M. Klinisch-gynäkologische und histopathologische Untersuchungen zur Beurteilung der endometrialen Reaktion auf besamungsrelevante Medien (Samenverdünner, Seminalplasma, Spermien) bei Warmblutstuten [Dissertation]. TiHo Hannover; 2001
    Google Scholar
  • 7 Christoffersen M, Baagoe CD, Jacobsen S. et al. Evaluation of the systemic acute phase response and endometrial gene ex-pression of serum amyloid A and pro- and antiinflammatory cytokines in mares with experimentally induced endometritis. Vet Immunol Immunopathol 2010; 138: 95-105
    CrossrefPubMedGoogle Scholar
  • 8 Woodward EM, Christoffersen M, Campos J, Betancourt A, Horohov D, Scoggin KE, Squires EL, Troedsson MH. Endometrial inflammatory markers of the early immune response in mares susceptible or resistant to persistent breeding-induced endometritis. Reproduction 2013; 145 (03) 289-296
    CrossrefPubMedGoogle Scholar
  • 9 Robertson SA, Sharkey DJ. Seminal fluid and fertility in women. Fertil Steril 2016; 106 (03) 511-519 doi:10.1016/j.fertnstert.2016.07.1101
    CrossrefPubMedGoogle Scholar
  • 10 Troedsson MH. et al. Equine seminal plasma derived lactoferrin regulates binding of polymorphonuclear neutrophils (PMNs) to spermatozoa. J Equine Vet Sci 2014; 34 (01) 1
    CrossrefGoogle Scholar
  • 11 Fedorka CE. et al. The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating-induced endometritis. Reprod Domest Anim 2017; 52 (01) 89e-96
    CrossrefPubMedGoogle Scholar
  • 12 Fedorka CE, Scoggin KE, Boakari YL, Hoppe NE, Squires EL, Ball BA, Troedsson MHT. The anti-inflammatory effect of exogenous lactoferrin on breeding-induced endometritis when administered post-breeding in susceptible mares. Theriogenol 2018; 114: 63-69 doi:10.1016/j.Theriogenol.2018.03.017
    CrossrefPubMedGoogle Scholar
  • 13 Ammons MC, Copie V. Mini-review: lactoferrin: a bioinspired, anti-biofilm therapeutic. Biofouling 2013; 29 (04) 443e-455
    CrossrefPubMedGoogle Scholar
  • 14 Carnevale EM, Ginther OJ. Relationships of age to uterine function and reproductive efficiency in mares. Theriogenol 1992; 37: 1101-1115
    CrossrefPubMedGoogle Scholar
  • 15 McKinnon AO, Squires EL, Carnevale EM. et al. Diagnostic ultrasonography of uterine pathology in the mare. Proc Am Assoc Equine Pract 1987; 33: 605-622
    Google Scholar
  • 16 Reilas T, Ristiniemi M, Katila T. Influence of hormone replacement therapy and bacterial inoculation on proteins and enzymes in uterine lavage fluid of ovariectomized mares. Reprod Domest Anim 1998; 33: 11-19
    CrossrefGoogle Scholar
  • 17 Brinsko SP, Rigby SL, Varner DD. et al. A practical method for recognizing mares susceptible to post-breeding endometritis. Proc Am Assoc Equine Pract 2003; 49: 363-365
    Google Scholar
  • 18 Rasmussen CD, Petersen MR, Bojesen HG. et al. Equine infectious endometritis–clinical and subclinical cases. J Equine Vet Sci 2015; 35: 95-104
    CrossrefGoogle Scholar
  • 19 Zent WW, Troedsson MH, Xue J. Postbreeding uterine fluid accumulation in a normal population of Thoroughbred mares: a field study. Proc Am Assoc Equine Pract 1998; 44: 64-65
    Google Scholar
  • 20 Troedsson MH, Madill S. Clinical examination of the reproductive tract of the mare. Proceedings of the Annual Conference, The Society for Theriogenol. Nashville, TN: 1999
    Google Scholar
  • 21 Dimock W, Edwards P. Bacteria of the genital tract of mares and the semen of stallions and their relation to breeding efficiency. J Am Vet Med Assoc 1923; 64: 288-298
    Google Scholar
  • 22 Riddle WT, LeBlanc MM, Stromberg AJ. Relationships between uterine culture, cytology and pregnancy rates in a Thoroughbred practice. Theriogenol 2007; 68: 395-402
    CrossrefPubMedGoogle Scholar
  • 23 Blanchard TL, Cummings MR, Garcia MC. et al. Comparison between two techniques for endometrial swab culture and between biopsy and culture in barren mares. Theriogenol 1981; 16: 541-552
    CrossrefPubMedGoogle Scholar
  • 24 Aguilar J, Hanks M, Shaw DJ. Importance of using guarded techniques for the preparation of endometrial cytology smears in mares. Theriogenol 2006; 66: 423-430
    CrossrefPubMedGoogle Scholar
  • 25 Spilker KC, Sielhorst J, Martinsson G. et al. Accuracy of different endometrial swabbing techniques in the mare. Pferdeheilkunde 2017; 2: 172-178
    Google Scholar
  • 26 Sielhorst J, Spilker K-C, Martinsson G, Hassler W, Burger D, Sieme H. Sensitivity and specificity of different endometrial swabbing techniques and their invasiveness and practicability in studfarm practice. Abstract 87, 51st Annual Conference on Physiology and Pathology of Reproduction Hannover 2018.
    Google Scholar
  • 27 LeBlanc MM, Causey RC. Clinical and subclinical endometritis in the mare: both threats to fertility. Reprod Domest Anim 2009; 44: 10-22
    CrossrefPubMedGoogle Scholar
  • 28 Christoffersen M, Brandis L, Samuelsson J, Bojesen AM, Troedsson MH, Petersen MR. Diagnostic double-guarded low-volume uterine lavage in mares. Theriogenol 2015; 83 (02) 222-227 doi:10.1016/j.Theriogenol.2014.09.008
    CrossrefPubMedGoogle Scholar
  • 29 Nielsen JM. Endometritis in the mare: a diagnostic study comparing cultures from swab and biopsy. Theriogenol 2005; 64: 510-518
    CrossrefPubMedGoogle Scholar
  • 30 Neuberg KP. Einsatz der exfoliativen Endometriumszytologie bei Zuchtstuten unter Praxisbedingungen: Vergleich von verschiedenen Entnahmemethoden [Dissertation]. Gießen: JLU Gießen; 2009
    Google Scholar
  • 31 Cochia N, Paciello O, Auletta L. et al. Comparison of the cytobrush, cottonswab, and low-volume uterine flush techniques to evaluate endometrial cytology for diagnosing endometritis in chronically infertile mares. Theriogenol 2012; 77: 89-98
    CrossrefPubMedGoogle Scholar
  • 32 Dascanio J. Endometrial cytology.In: Robinson NE, Hrsg. Current therapy in equine medicine. Philadelphia, USA: WB Saunders Co; 2003: 226-228
    Google Scholar
  • 33 Ferris RA, Bohn AA, McCue PM. Equine endometrial cytology: Collection techniques and interpretation. Equine Vet Educ 2015; 27: 316-322
    CrossrefGoogle Scholar
  • 34 LeBlanc M. Uterine cytology. In: McKinnon AO, Squires E, Vaala WE, Varner DD. Hrsg. . Equine Reproduction Ames, IA, USA: Wiley-Blackwell 2011; 1922.  –  1928
    Google Scholar
  • 35 Kozdrowski R, Sikora M, Buczkowska J. et al. Effects of cycle stage and sampling procedure on interpretation of endometrial cytology in mares. Anim Reprod Sci 2015; 154: 56-62
    CrossrefPubMedGoogle Scholar
  • 36 Nielsen JM, Troedsson MH, Pedersen MR. et al. Diagnosis of endometritis in the mare based on bacteriological and cytological examinations of the endometrium: comparison of results obtained by swabs and biopsies. J Equine Vet Sci 2010; 30: 27-30
    Google Scholar
  • 37 Burleson MD, LeBlanc MM, Riddle WT. et al. Endometrial microbial isolates are associated with different ultrasonographic and endometrial cytology findings in Thoroughbred mares. Proc Am Assoc Equine Pract 2010; 56: 317
    Google Scholar
  • 38 Katila T. Evaluation of diagnostic methods in equine endometritis. Reproductive Biology 2016; 16: 189-196
    CrossrefPubMedGoogle Scholar
  • 39 Schoon HD, Schoon D, Klug E. Die Endometriumbiopsie bei der Stute im klinisch-gynäkologischen Kontext. Pferdeheilkunde 1997; 13: 453-464
    Google Scholar
  • 40 Kenney RM. Prognostic value of endometrial biopsy of the mare. J Reprod Fertil Suppl 1975; 23: 347-348
    Google Scholar
  • 41 Ricketts SW, Alonso S. Assessment of the breeding prognosis of mares using paired endometrial biopsy techniques. Equine Vet J 1991; 23: 185-188
    CrossrefPubMedGoogle Scholar
  • 42 Otzen H, Sieme H, Oldenhof H, Ertmer F, Kehr A, Rode K, Klose K, Rohn K, Schoon HA, Meinecke B. Identification of vessel degeneration and endometrosis in the equine endometrium, using narrow-band imaging hysteroscopy. Theriogenol 2016; 86 (06) 1445-1452 doi:10.1016/j.Theriogenol.2016.05.002
    CrossrefPubMedGoogle Scholar
  • 43 Bartmann CP, Schöning A, Brickwedel B, Ohnesorge B, Klug E. Hysteroskopie und minimal invasive endouterine Chirurgie bei der Stute. Pferdeheilkunde 1997; 13: 474-482
    Google Scholar
  • 44 Inoue Y, Sekiguchi M. Clinical application of hysteroscopic hydrotubation for unexplained infertility in the mare. Equine Vet J 2018; 50 (04) 470-473 doi:10.1111/evj.12781
    CrossrefPubMedGoogle Scholar
  • 45 Tönissen A, Müller-Stephan L, Tiedemann D, Rau J, Sieme H. An approach to hysteroscopic catheterization of the equine oviduct. 1st ECAR Symposium Wien. 2019
    Google Scholar
  • 46 Vanderwall DK, Woods GL. Effect on fertility of uterine lavage performed immediately prior to insemination in mares. J Am Vet Med Assoc 2003; 222: 1108-1110
    CrossrefPubMedGoogle Scholar
  • 47 Campbell MLH, England GCW. A comparison of the ecbolic efficacy of intravenous and intrauterine oxytocin treatments. Theriogenol 2002; 58: 473-477
    CrossrefGoogle Scholar
  • 48 Combs GB, LeBlanc MM, Neuwirth L. et al. Effects of prostaglandin F-2α cloprostenol and fenprostalene on uterine clearance of radiocolloid in the mare. Theriogenol 1996; 45: 1449-1455
    CrossrefGoogle Scholar
  • 49 Käser R, Bruckmaier RM, Thomas S, Sieme H, Wedekind C, Burger D. High interindividual and intraindividual variation of oxytocin secretion in estrous mares exposed to stallions, but no significant link to mate preferences. Theriogenol 2016; 86 (09) 2222-2229
    CrossrefPubMedGoogle Scholar
  • 50 Klewitz J, Großheim B, Schwenzer K, Pansegrau U. et al. Effects of topical medications on the dimensions of the cervix in estrous mares. Abstract 2014. XIth International Symposium on Equine Reproduction, Hamilton, New Zealand.
    Google Scholar
  • 51 Pycock JF, Newcombe JR. Assessment of the effect of three treatments to remove intrauterine fluid on pregnancy rate in the mare. Vet Rec 1996; 138: 320-323
    CrossrefPubMedGoogle Scholar
  • 52 Brinsko SP. Common procedures in broodmare practice: what is the evidence?. Vet Clin Equine 2007; 23: 385-402
    CrossrefPubMedGoogle Scholar
  • 53 Rose BV, Firth M, Morris B, Roach JM, Wathes DC, Verheyen KLP, de Mestre AM. Descriptive study of current therapeutic practices, clinical reproductive findings and incidence of pregnancy loss in intensively managed thoroughbred mares. Anim Reprod Sci 2018; 188: 74-84 doi:10.1016/j.anireprosci.2017.11.011
    CrossrefPubMedGoogle Scholar
  • 54 Cooke CD. Prophylactic intra-uterine antibacterial therapy. Equine Vet Educ 2015; 27: 554-555
    CrossrefGoogle Scholar
  • 55 Dascanio JJ. How and when to treat endometritis with systemic or local antibiotics. Proc Am Assoc Equine Pract 2011; 57: 24-31
    Google Scholar
  • 56 Davolli GM, Beavers KN, Medina V. et al. Concentrations of Sulfadiazine and Trimethoprim in Blood and Endometrium of Mares After Administration of an Oral Suspension. J Equine Vet Sci 2018; 67: 27-30
    CrossrefGoogle Scholar
  • 57 Murchie TA, Macpherson ML, LeBlanc MM, Luznar S, Vickroy TW. Continuous monitoring of penicillin G and gentamicin in allantoic fluid of pregnant pony mares by in vivo microdialysis. Equine Vet J 2006; 38 (06) 520-525
    CrossrefPubMedGoogle Scholar
  • 58 Witte TS, Hahn K, Duerr S. Concentrations of Gentamicin in serum, intrauterine fluid and endometrial tissue after intravenous administration in healthy mares. J Equine Vet Sci 2018; 66: 115
    CrossrefGoogle Scholar
  • 59 LeBlanc MM, McKinnon AO. Breeding the problem mare. In: McKinnon AO, Squires EL, Vaala WE. et al., Hrsg. Equine Reproduction. West Sussex: Wiley-Blackwell; 2011
    Google Scholar
  • 60 Ferris RA, McCue P. Comparison of Ceftiofur, tris-EDTA or Combination in Treatment of Biofilm Associated Pseudomonas Infections. AAEP 2018; 64
    Google Scholar
  • 61 Ferris RA, McCue P, Borlee GI, Loncar KD, Hennet ML, Borlee BR. In vitro efficacy of Nonantibiotic treatments of biofilm disruption of gram-negative pathogens and an in vivo model of infectious endometritis utilizing isolates from the equine uterus. J Clin Microb 2016; 54: 631-639
    CrossrefPubMedGoogle Scholar
  • 62 Troedsson MHT, Nielsen JM. Non-antibiotic treatment of equine endometritis. Pferdeheilkunde 2018; 34 (01) 17-22
    Google Scholar
  • 63 Emmerich I. Arzneimittelanwendung bei Pferden. Vetidata; 2018
    Google Scholar
  • 64 Melkus E, Witte T, Walter I, Heuwieser W, Aurich C. Investigations on the Endometrial Response to Intrauterine Administration of N-Acetylcysteine in Oestrous Mares. Reprod Dom Anim 2013; 48: 591-597
    CrossrefPubMedGoogle Scholar
  • 65 Petersen MR, Skive B, Christoffersen M, Lu K, Nielsen JM, Troedsson MHT, Bojesen AM. Activation of persistent Streptococcus equi subspecies zooepidemicus in mares with subclinical endometritis. Vet Microbiol 2015; 179: 119-125.2015.06.006 doi:10.1016/jvetmic
    CrossrefPubMedGoogle Scholar
  • 66 Woodward EM, Christoffersen M, Horohov D, Squires EL, Troedsson MH. The effect of treatment with immune modulators on endometrial cytokine expression in mares susceptible to persistent breeding-induced endometritis. Equine Vet J 2015; 47 (02) 235-239 doi:10.1111/evj.12266
    CrossrefPubMedGoogle Scholar
  • 67 Bucca S, Carli A, Buckley T, Dolci G, Fogarty U. The use of dexamethasone administered to mares at breeding time in the modulation of persistent mating induced endometritis. Theriogenol 2008; 6: 1093-1100
    Google Scholar
  • 68 Ferris RA, Frisbie DD, McCue PM. Use of mesenchymal stem cells or autologous conditioned serum to modulate the inflammatory response to spermatozoa in mares. Theriogenol 2014; 82 (01) 36-42 doi:10.1016/j.Theriogenol.2014.02.015
    CrossrefPubMedGoogle Scholar
  • 69 Metcalf ES. The effect of platelet-rich plasma (PRP) on intraluminal fluid and pregnancy rates in mares susceptible to persistent mating-induced endometritis. J Equine Vet Sci 2014; 34: 128
    CrossrefGoogle Scholar