Suppressive Effects of Hot-water Extract of Magnolia obovata on Clostridium perfringens Enterotoxin-induced Cytotoxicity in Human Intestinal Caco-2 Cells

Takeshi Kawahara; Kotomi Fujii; Kouki Nakajima; Rikuri Fujii; Shuichiro Inagaki; Kunio Hara; Hisako Yasui

doi:10.1055/a-1078-7860

Planta Medica, Table of Contents

Planta Med 2020; 86(03): 198-204
DOI: 10.1055/a-1078-7860

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Suppressive Effects of Hot-water Extract of Magnolia obovata on Clostridium perfringens Enterotoxin-induced Cytotoxicity in Human Intestinal Caco-2 Cells

Authors

Takeshi Kawahara

¹Academic Assembly School of Science and Technology, Institute of Agriculture, Shinshu University
Kotomi Fujii

¹Academic Assembly School of Science and Technology, Institute of Agriculture, Shinshu University
Kouki Nakajima

¹Academic Assembly School of Science and Technology, Institute of Agriculture, Shinshu University
Rikuri Fujii

¹Academic Assembly School of Science and Technology, Institute of Agriculture, Shinshu University
Shuichiro Inagaki

²Department of Health and Nutrition, Faculty of Health and Nutrition, Osaka Shoin Womenʼs University
Kunio Hara

³Kiso Town Resources Institute
Hisako Yasui

³Kiso Town Resources Institute

Abstract

The physiological functions of the leaves of Japanese big-leaf magnolia (Magnolia obovata) against enterotoxins produced by foodborne pathogens remain unclear. In this study, we evaluated the protective effects of M. obovata leaf extract (MLE) against the cytotoxicity of Clostridium perfringens enterotoxin (CPE), which causes the symptoms of C. perfringens type A food poisoning. The protective effects of MLE against CPE-induced cytotoxicity were evaluated in human intestinal epithelial Caco-2 cells. Pre-treatment with MLE significantly suppressed the cytotoxicity induced by CPE in undifferentiated and differentiated human intestinal Caco-2 cells at a pH range of 4.0 – 7.0. This CPE-suppressive effect was due to a hydrophilic sugar-containing compound without phenolic and protein structures but not the hydrophobic biologically active neolignans, honokiol and magnolol. MLE had a protective effect against cytotoxicity caused by type A C. perfringens. Our results provide novel insight regarding the usage of M. obovata in managing food poisoning.

Key words

Magnolia obovata - Magnoliaceae - Clostridium perfringens - enterotoxin - food poisoning - claudin

Full Text

References

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