Planta Med 2020; 86(04): 294-302
DOI: 10.1055/a-1088-8449
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

In Vitro and In Vivo Activity of Anogeissus leiocarpa Bark Extract and Isolated Metabolites against Toxoplasma gondii

Jérémy Spalenka#
1   Université de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France
2   Université de Reims Champagne Ardenne, ESCAPE EA 7510, Reims, France
,
Jane Hubert##
1   Université de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France
5   NatExplore SAS, Prouilly, France
,
Laurence Voutquenne-Nazabadioko
1   Université de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France
,
Sandie Escotte-Binet
2   Université de Reims Champagne Ardenne, ESCAPE EA 7510, Reims, France
,
Nicolas Borie
1   Université de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France
,
Frédéric Velard
3   Université de Reims Champagne Ardenne, BIOS, EA 4691, Reims, France
,
Isabelle Villena
2   Université de Reims Champagne Ardenne, ESCAPE EA 7510, Reims, France
4   Centre National de Référence de la Toxoplasmose, CHU Hôpital Maison Blanche, Reims, France
,
Dominique Aubert
2   Université de Reims Champagne Ardenne, ESCAPE EA 7510, Reims, France
4   Centre National de Référence de la Toxoplasmose, CHU Hôpital Maison Blanche, Reims, France
,
Jean-Hugues Renault
1   Université de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, Reims, France
› Author Affiliations
Supported by: CHU Robert Debré Reims Co-funding PhD thesis
Supported by: Conseil Régional Champagne Ardenne co-funding PhD thesis
Supported by: Société d'Accélération du Transfert de Technologie Nord
Further Information

Publication History

received 20 September 2019
revised 08 December 2019

accepted 31 December 2019

Publication Date:
28 January 2020 (online)

Abstract

Toxoplasma gondii, belonging to the Apicomplexa phylum, is a cosmopolitan protozoan parasite that affects at least 30% of the worldʼs population. In West Africa, the leaves and bark of the tree species Anogeissus leiocarpa (DC.) Guill. & Perr. are used against zoonosis in traditional medicine and play a key role in controlling diseases induced by Apicomplexans such as malaria. In this study, extracts, fractions, and pure compounds obtained from an ethanol extract of the bark of A. leiocarpa were evaluated against T. gondii infection in vitro and in vivo. The crude bark extract showed significant activity on tachyzoites from the T. gondii RH strain (IC50 = 59.30 µg/mL). The crude bark extract without tannins and pure trachelosperogenin E purified by centrifugal partition chromatography showed the highest activity (IC50s = 12.83 and 26.63 µg/mL, respectively) with satisfying selectivity indexes of 9.61 and 9.75, respectively. The crude bark extract without tannins and pure trachelosperogenin E were able to significantly inhibit host cell invasion by the parasite in vitro, while the crude bark extract without tannins was able to increase mice survival in our murine model of chronic toxoplasmosis. These results provide new biological data for natural compounds that could enhance the current panoply of treatments against toxoplasmosis.

# Current Address: Université de Reims Champagne Ardenne, CNRS, MEDyC UMR 7369, Reims, France


## Current Address: NatExplore SAS, Prouilly, France


Supporting Information

 
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