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DOI: 10.1055/a-1088-8449
In Vitro and In Vivo Activity of Anogeissus leiocarpa Bark Extract and Isolated Metabolites against Toxoplasma gondii
Supported by: CHU Robert Debré Reims Co-funding PhD thesisSupported by: Conseil Régional Champagne Ardenne co-funding PhD thesis
Supported by: Société d'Accélération du Transfert de Technologie Nord
Publication History
received 20 September 2019
revised 08 December 2019
accepted 31 December 2019
Publication Date:
28 January 2020 (online)
Abstract
Toxoplasma gondii, belonging to the Apicomplexa phylum, is a cosmopolitan protozoan parasite that affects at least 30% of the worldʼs population. In West Africa, the leaves and bark of the tree species Anogeissus leiocarpa (DC.) Guill. & Perr. are used against zoonosis in traditional medicine and play a key role in controlling diseases induced by Apicomplexans such as malaria. In this study, extracts, fractions, and pure compounds obtained from an ethanol extract of the bark of A. leiocarpa were evaluated against T. gondii infection in vitro and in vivo. The crude bark extract showed significant activity on tachyzoites from the T. gondii RH strain (IC50 = 59.30 µg/mL). The crude bark extract without tannins and pure trachelosperogenin E purified by centrifugal partition chromatography showed the highest activity (IC50s = 12.83 and 26.63 µg/mL, respectively) with satisfying selectivity indexes of 9.61 and 9.75, respectively. The crude bark extract without tannins and pure trachelosperogenin E were able to significantly inhibit host cell invasion by the parasite in vitro, while the crude bark extract without tannins was able to increase mice survival in our murine model of chronic toxoplasmosis. These results provide new biological data for natural compounds that could enhance the current panoply of treatments against toxoplasmosis.
Key words
Toxoplasma gondii - Anogeissus leiocarpa - Combretaceae - centrifugal partition chromatography - natural products - chemosensitivity# Current Address: Université de Reims Champagne Ardenne, CNRS, MEDyC UMR 7369, Reims, France
## Current Address: NatExplore SAS, Prouilly, France
Supporting Information
- Supporting Information
13C NMR assignments of A. leiocarpa metabolites as well as cytotoxic activity of the crude bark extract and optical microscopic observation of the RH strain of T. gondii growth in the presence of each compound/fraction from A. leiocarpa are available as Supporting Information.
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