Anti-lipidemic Effect of Fractions of Peristrophe bivalvis Leaf in NG-nitro-L-arginine Methyl Ester (L-NAME) Treated Rats
received 02 February 2020
accepted 09 March 2020
03 April 2020 (online)
Purpose The reduction in nitric oxide (NO) bioavailability accelerates atherosclerosis development, augments lipolysis, elevates blood pressure and upregulate leukocyte level. This study was designed to examine the biochemical constituents of fractions of Peristrophe bivalvis (PB) leaf, their effect on blood pressure, serum lipid contents and complete blood count in NG-nitro-L-arginine methyl ester (L-NAME) hypertensive rats.
Method Male Wistar rats were grouped into control and hypertensive groups. The hypertensive group was pretreated with 60 mg/kg b.w of L-NAME (L-NAME60) daily for two weeks. They were then randomly sub-grouped into: Hypertensive (H), Hypertensive+n-hexane fraction (HHF), Hypertensive+dichloromethane fraction (HDF), Hypertensive+ethyl acetate fraction (HEF) and Hypertensive+aqueous fraction (HAF) groups. These were orally gavaged with L-NAME60 and L-NAME60+200 mg/kg b.w of fractions of PB respectively, daily for two weeks.
Result The biochemical components analysis of the fractions of PB identified numerous polar and non polar compounds like alkaloids, organic acids and esters. The results showed a significant increase in NO level in HHF and HEF groups compared to H. Total cholesterol, triglyceride, low density lipoprotein cholesterol (LDL-C) and very LDL-C were significantly decreased in HAF group compared to H. High density lipoprotein cholesterol (HDL-C) increased significantly and atherogenic indices decreased significantly in HHF, HDF and HAF groups compared to H, while reduced glutathione level increased significantly in HHF group compared to H. White blood cells count effectively decreased in HEF group compared to H.
Conclusion In brief, the fractions of PB leaf increased HDL-C and NO, and decrease atherogenic indices in L-NAME treated rats.
Keywordsnitric oxide synthase inhibitor - Peristrophe bivalvis - nitric oxide - lipid contents - blood pressure
* Esther Oluwasola Aluko designed the work and drafted the manuscript.
† Temidayo Olutayo Omobowale and Ademola Adetokunbo Oyagbemi coordinated and measured the blood pressure of the animals.
‡ Adesoji Adedipe Fasanmade revised and approved the final version of the manuscript.
- 1 Zhang Y, Gan R, Li S. et al. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules 2015; 20: 21138-21156
- 2 Dang BK, Dam SM, Pham MT. et al. Peristrophe roxburghiana - A review. AFST 2014; 15: 1-9
- 3 Do TX, Nguyen TPT. Some results of study on Peristrophe bivalvis (Acanthaceae), The 2nd National Scientific Conference on Ecological and Biological Resources, Ha Noi 2007; 292-294
- 4 Trinh TT, Nguyen TTH, Le THN. et al. Isolation, characterisation and biological evaluation of a phenoxazine, a natural dyestuff isolated from leaves of Peristrophe bivalvis. Nat Prod Res 2013; 27: 771-774
- 5 Jiaju Z, Guirong X, Xinjian Y. Encyclopedia of Traditional Chinese Medicines molecular structures, pharmacological activities, natural sources and application isolated compounds T-Z References, TCM Plants and Congeners. New York: Springer-Verlag Berlin Heidelberg; 2011. 5 60-61
- 6 Zhuang X, Lü J, Yang W. et al. Effects of Peristrophe roxburghiana on blood pressure NO and ET in renal hypertensive rats. J Chinese Med Materials 2003; 26: 266-268
- 7 Cheng Z, Lü J, Liu J. Effects of Peritrophe roxburghiana on Blood Pressure in Renal Hypertensive and Hyperlipidemic Rats. J Chinese Medicinal Materials 2004; 27: 927-930
- 8 Yang W, Gu F, Lü J. et al. Effect of the extract from Peristrophe roxburghiana on hemorheology in rats. J Chinese Med Materials 2002; 25: 727-728
- 9 Qin SS, Liu XF, Zhang KF. The liver-protective effect of polysaccharide from Peristrophe roxburghiana. West China Journal of Pharmaceutical Sciences 2010; 5: 559-560
- 10 Nelson RH. Hyperlipidemia as a risk factor for cardiovascular disease. Primary Care 2013; 40: 195-211 doi:10.1016/j.pop.2012.11.003.
- 11 Matsuzawa Y, Lerman A. Endothelial dysfunction and coronary artery disease, assessment, prognosis, and treatment. Coronary Artery Disease 2014; 25: 713-724
- 12 Förstermann U, Xia N, Li H. Roles of vascular oxidative stress and nitric oxide in the pathogenesis of atherosclerosis. Circ Res 2017; 120: 713-735
- 13 Niang F, Benelli C, Ribie’re C. et al. Leptin induces nitric oxide-mediated inhibitionof lipolysis and glyceroneogenesis in rat white adipose tissue1–3. J. Nutr 2011; 141: 4-9
- 14 Salami SA, Hussein MS, Omotoke CR. et al. Oral administration of Tridax procumbens aqueous leaf extract attenuates reproductive function impairments in L-NAME induced hypertensive male rats. Middle East Fertil Soc J 2017; 22: 219-225
- 15 Goudarz S, Mohaddeseh A, Siamak A. Role of nitric oxide in the plasma lipid profile in the rabbits. Arch Med Sci 2009; 5: 308-312
- 16 Murugesan S, Raja B. Effect of veratric acid on the cardiovascular risk of L-NAME-induced hyperlipidemic rats. J Cardiovasc Pharmacol 2012; 59: 553-569
- 17 Harborne JB. Phytochemical Methods - A Guide To Modern Techniques of Plant Analysis. 2nd ed. London: Chapman and Hall; 1984. pp 4-16
- 18 Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT 1995; 28: 25-30
- 19 Prieto P, Pineda M, Anguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex. Specific application to the determination of Vitamin E. Anal. Biochem 1999; 269: 337-341
- 20 Rajeswari G, Murugan M, Mohan VR. 2012; GC-MS analysis of bioactive components of Hugonia mystax L. (Linaceae). RJPBCS 34: 301-308
- 21 Qadir A, Singh SP, Akhtar J. Phytochemical and GC-MS analysis of Saudi Arabian Ajwa variety of date seed oil and extracts obtained by the slow pyrolysis method. Oriental Pharmacy and Experimental Medicine 2017; 17: 81-87
- 22 Mingorance C, Ariantsitohaina R, Sotomayor M. Cedrelopsis grevei improves endothelial vasodilatation in aged rats through an increase of NO participation. J Ethanopharmacology 2008; 117: 76-83
- 23 Ohkawa H, Ohishi N, Yagi K. Assay for Lipid peroxidation and lipid peroxides in animal tissues by thiobarbituric. acid reaction. Anal Biochem 1979; 95: 351-358
- 24 Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 61: 882-890
- 25 Ojiako AO, Chikezie PC, Zedech UC. Serum lipid profile of hyperlipidemic rabbits (Lepustownsendii) administered with leaf extracts of Hibiscus rosesinesis, Emilia coccinea, Acanthus montanus and Asystasia gangetica. J Med Plant Res 2013; 7: 3226-3231
- 26 Friedewald W, Levy R, Fredrickson D. Estimation of concentration of lowdensity lipoprotein in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499-502
- 27 Brandes RP. Endothelial dysfunction and hypertension. Hypertens 2014; 64: 924-928
- 28 Kazuki N, Shigeo G, Hiroki S. et al. Opposing roles of nitric oxide and rho-kinase in lipid metabolism in mice. Tohoku J Exp Med 2015; 235: 171-183
- 29 Fengmei Z, Bin D, Baojun X. Anti-inflammatory effects ofphytochemicals from fruits, vegetables, and food legumes, A review, Critical Reviews in Food Science and Nutrition. 2017 DOI: 10.1080/10408398.2016.1251390.
- 30 Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000; 63: 1035-1042
- 31 Murni NS, Qamar UA, Siti ZM. 2017. “Antioxidant and Antidiabetic Effects of Flavonoids, A Structure-Activity Relationship Based Study”. Bio Med Research International 2017; Article ID 8386065
- 32 Cortijo J, Villagrasa V, Pons R. Bronchodilator and anti-inflammatory activities of glaucine, In vitro studies in human airway smooth muscle and polymorphonuclear leukocytes. British Journal of Pharmacology 1999; 127: 1641-1651
- 33 Elks J. The Dictionary of Drugs, Chemical Data, Chemical Data, Structures and Bibliographies. Springer; 2014: 162-163
- 34 Kee VR. Hemodynamic pharmacology of intravenous vasopressors. Crit Care Nurse 2003; 23: 79-82
- 35 Herrera-Arellano A, Jiménez-Ferrer JE, Zamilpa A. et al. Therapeutic effectiveness of Galphimia glauca vs. lorazepam in generalized anxiety disorder. A controlled 15-week clinical trial. Planta Med 2012; 78: 1529-1535
- 36 Aluko EO, Adejumobi OA, Fasanmade AA. Peristrophe roxburghiana leaf extracts exhibited anti-hypertensive and anti-lipidemic properties in L-NAME hypertensive rats. Life Sciences 2019; 234: 116753
- 37 Singh BK, Mehta JL. Management of dyslipidemia in the primary prevention of coronary heart disease. Curr Opinion Cardiol 2002; 17: 503-511
- 38 Parinita K. Study of serum lipid profile in individuals residing in and around Nalgonda. Int J pharm Bio Sci 2012; 2: 110-116
- 39 Ras RT, Geleijnse JM, Trautwein EA. LDL-cholesterol-lowering effect of plant sterols and stanols across different dose ranges, a meta-analysis of randomised controlled studies. Br J Nutr 2014; 112: 214-219
- 40 Martins FTM, Noso VB, Porto A. et al. Maté tea inhibits in vitro pancreatic lipase activity and has hypolipidemic effect on highfat diet-induced obese mice. Obesity 2010; 18: 42-47
- 41 Marrelli M, Conforti F, Araniti F. et al. Effects of saponins on lipid metabolism, a review of potential health benefits in the treatment of obesity. Molecules 2016; 20 21(10). pii E1404
- 42 Selamoglu Talas Z. 2014. Propolis reduces oxidative stress in l-NAME-induced hypertension rats. Cell Biochem Funct 2014; 32: 150-154
- 43 Malarvili T, Veerappan R. Effects of chrysin on free radicals and enzymatic antioxidants in Nω-nitro-l-arginine methyl ester: Induced hypertensive rats. Int J Nutr Pharmacol Neurol Dis 2014; 4: 112-117
- 44 Santosh Venkat Ramesh. Nitric Oxide – “Double Edged Sword” – Review. Trends in Biomaterials and Artificial Organs 2014; 28: 37-43
- 45 Abdel-Salam OmarM.E., Youness EmanR., Mohammed NadiaA.. et al. Nitric oxide synthase inhibitors protect against brain and liver damage caused by acute malathion intoxication. Asian Pacific Journal of Tropical Medicine 10: 773-786
- 46 Dickinson DA, Forman HJ. Glutathione in defense and signaling, Lessons from a small thiol. Ann NY Acad Sci 2002; 973: 488-504
- 47 Evans JL, Goldfine ID, Maddux BA. et al. Oxidative stress and stress-activated signaling pathways, A unifying hypothesis of type 2 diabetes. Endocr Rev 2002; 23: 599-622
- 48 Hossain Mokarram, Qadri SyedM, Liu Lixin. Inhibition of nitric oxide synthesis enhances leukocyte rolling and adhesion in human microvasculature. J Inflamm (Lond) 2012; 9: 28
- 49 Farhangi MA, Keshavarz S, Eshraghian M. et al. White blood cell count in women, relation to inflammatory biomarkers, haematological profiles, visceral adiposity, and other cardiovascular risk factors. J Health Popul Nutr 2013; 31: 58-64
- 50 Kubes P, Suzuki M, Granger DN. Nitric oxide: An endogenous modulator of leukocyte adhesion. Proc Natl Acad Sci USA 1991; 88: 4651-4655