Regulation of Circulatory Muscle-specific MicroRNA during 8 km
                    Run

Xin Yin; Shufang Cui; Xin Li; Wei Li; Qiu ju Lu; Xiao hong Jiang; Hui Wang; Xi Chen; Ji zheng Ma

doi:10.1055/a-1145-3595

International Journal of Sports Medicine, Table of Contents

Int J Sports Med 2020; 41(09): 582-588
DOI: 10.1055/a-1145-3595

Physiology & Biochemistry

Regulation of Circulatory Muscle-specific MicroRNA during 8 km Run

Authors

Xin Yin

¹School of life sciences, Nanjing University, Nanjing, China
Shufang Cui

¹School of life sciences, Nanjing University, Nanjing, China
Xin Li

¹School of life sciences, Nanjing University, Nanjing, China
Wei Li

²The Research Center of Military Exercise Science, The Army Engineering University of PLA, Nanjing, China
Qiu ju Lu

²The Research Center of Military Exercise Science, The Army Engineering University of PLA, Nanjing, China
Xiao hong Jiang

¹School of life sciences, Nanjing University, Nanjing, China
Hui Wang

³Department of Ultrasound Diagnosis, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China
Xi Chen

¹School of life sciences, Nanjing University, Nanjing, China
Ji zheng Ma

²The Research Center of Military Exercise Science, The Army Engineering University of PLA, Nanjing, China

Abstract

Acute prolonged endurance running has been shown to alter muscle-specific circulating microRNA (miRNA) levels. Here, eighteen participants completed an 8 km run. We assessed the levels of hsa-miR-1–3p, -133a-3p, -133b, and -206 and their correlation with conventional biomarkers following exercise. Compared to before exercise (Pre), 8 km run significantly increased the lactate level immediately after exercise (0 h). Myoglobin (Mb) level increased at 0 h while creatine kinase (CK) level increased 24 h after exercise (24 h). The levels of creatine kinase MB isoenzyme (CK-MB) and cardiac troponin I (cTnI) were all elevated at 24 h and within the normal physiological range; The levels of hsa-miR-1–3p, -133a-3p, -133b significantly increased at 0 h but only hsa-miR-133a-3p still elevated at 24 h. Only hsa-miR-206 level decreased at 24 h; Additionally, the changes of hsa-miR-1–3p and hsa-miR-133a-3p were correlated with Mb at 24 h. These findings suggest that muscle-specific miRNA elevation in plasma is likely physiological and that these miRNA may be used as potential biomarkers for load monitoring in individuals.

Key words

internal load - exercise - circulating microRNAs - autonomic nervous system - plasma

Full Text

References

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