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DOI: 10.1055/a-1176-1884
Daucosterol linolenate from Sweet Potato Suppresses MCF7-Xenograft-Tumor Growth through Regulating PI3K/AKT Pathway
Gefördert durch: Industrial Technology System Program for Traditional Chinese Herbs of Chongqing Municipal Agricultural Commission 2017[5]Gefördert durch: Chongqing postgraduate research and research innovation project CYB18087
Gefördert durch: Achievement Transfer Program of Institutions of Higher Education in Chongqing KJZH17105
Gefördert durch: The special program for Scientific and Technical Innovation of Chongqing Social Livelihood cstc2017shms-kjfp80004
Publikationsverlauf
received 09. Oktober 2019
revised 25. April 2020
accepted 05. Mai 2020
Publikationsdatum:
08. Juni 2020 (online)
Abstract
Sweet potato is a functional food with potential antitumor properties, but the bioactive constituents and biological mechanisms remain unclear. In this study, we investigated the antitumor effect of daucosterol linolenate extracted from sweet potato and its potential mechanism. An MTT assay indicated that DLA inhibited the proliferation of breast cancer MCF-7 cells but had only weak effects on the proliferation of MDA-MB-231, 4T1, and MCF-10A cells. Flow cytometry analysis revealed that daucosterol linolenate induced apoptosis of MCF-7 cells. Experiments with MCF-7 xenograft in nude mice further confirmed that DLA inhibited tumor growth dose-dependently. After DLA treatment, the expressions of B-cell lymphoma 2 and vascular endothelial growth factor were decreased and that of cleaved caspase 3 was increased as compared to the TC group. DLA also down-regulated the expression of phosphoinositide 3-kinase/protein kinase B and repressed insulin-induced phosphoinositide 3-kinase/protein kinase B activation. Our findings suggest that DLA suppresses breast tumor growth through inactivating the phosphoinositide 3-kinase/protein kinase B pathway.
Key words
Ipomoea batatas - Convolvulaceae - Daucosterol linolenate - breast cancer - phosphoinositide 3-kinase/protein kinase BSupporting Information
- Supporting Information
Additional information about 13C NMR, 1H NMR, mass spectra, GC-MS, and the relative expression data of ER and cleaved caspase 7 are available as Supporting Information.
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