Method Development and Validation of UV Spectrophotometric Method for the Quantitative Estimation of Curcumin in Simulated Nasal Fluid
Background Curcumin is a polyphenolic compound with numerous therapeutic activities. There is no validated method available for the quantitative estimation of curcumin in simulated nasal fluid.
Objective The aim of present investigation was to develop a simple and precise UV visible spectrophotometric method for estimation of pure form of curcumin in simulated nasal fluid.
Method Suitable solvent system was selected by estimation of curcumin at UV maxima of 421nm in simulated nasal fluid with two surfactants (tween 80 and sodium lauryl sulphate). The double beam UV visible spectrophotometer was used for measurement of absorption. The selected solvent system was further validated according to guidelines of international conference on harmonization (ICH), the analytical parameter like linearity, precision and accuracy etc. were studied.
Results Simulated nasal fluid with tween 80 at 1% concentration satisfied all the conditions relative to Peak quality at the stated wavelength. In developed method, curcumin was found to be linear over selected concentration range of 5 to 60µg/ml with a correlation coefficient of 0.998. The accuracy was found to be in range of 99.51 –100.223%.The precision was found to be less than 2 in terms of % RSD. The LOD & LOQ were 0.3657 & 1.109 respectively.
Conclusion The proposed method was found to be simple, sensitive and precise. The most important this method can be used for routine quality control analysis of curcumin with accuracy.
Received: 09 April 2020
Accepted: 02 June 2020
23 June 2020 (online)
© Georg Thieme Verlag KG
Stuttgart · New York
- 1 Prado-Audelo D, María L, Caballero-Florán IH. et al. Formulations of curcumin nanoparticles for brain diseases. Biomolecules 2019; 9: 56
- 2 Potter PE. Curcumin: A natural substance with potential efficacy in Alzheimer’s disease. J. Exp. Pharmacol., 2013, 5, 23. Potter PE. Curcumin: A natural substance with potential efficacy in Alzheimer’s disease. J Exp Pharmacol 2013; 5: 23
- 3 Lee WH, Loo CY, Bebawy M. et al. Curcumin and its derivatives: Their application in neuropharmacology and neuroscience in the 21st century. Curr Neuropharmacol 2013; 11: 338-378
- 4 Anusha D, Chaly PE, Junaid M. et al. Efficacy of a mouthwash containing essential oils and curcumin as an adjunct to nonsurgical periodontal therapy among rheumatoid arthritis patients with chronic periodontitis: A randomized controlled trial. Indian J Dent Res 2019; 30: 506
- 5 Rainey NE, Moustapha A, Saric A. et al. Iron chelation by curcumin suppresses both curcumin-induced autophagy and cell death together with iron overload neoplastic transformation. Cell Death Discov 2019; 5: 1-5
- 6 Tomeh MA, Hadianamrei R, Zhao X. A review of curcumin and its derivatives as anticancer agents. Int J Mol Sci 2019; 20: 1033
- 7 Yavarpour-Bali H, Ghasemi-Kasman M, Pirzadeh M. Curcumin-loaded nanoparticles: A novel therapeutic strategy in treatment of central nervous system disorders. Int J Nanomedicine 2019; 14: 4449
- 8 Hotsumi M, Tajiri M, Nikaido Y. et al. Design, synthesis, and evaluation of a water soluble C5-monoketone type curcumin analogue as a potent amyloid β aggregation inhibitor. Bioorg Med Chem Lett. 2019; 29: 2157-2161
- 9 Nelson KM, Dahlin JL, Bisson J. et al. The essential medicinal chemistry of curcumin: miniperspective. J Med Chem. 2017; 60: 1620-1637
- 10 Reddy PH, Manczak M, Yin X. et al. Protective effects of Indian spice curcumin against amyloid-β in Alzheimer’s disease. J Alzheimers Dis. 2018; 61: 843-866
- 11 Sharma K, Agrawal SS, Gupta M. Development and validation of UV spectrophotometric method for the estimation of curcumin in bulk drug and pharmaceutical dosage forms. Int J Drug Dev Res 2012; 4: 375-380
- 12 Kharat M, McClements DJ. Recent advances in colloidal delivery systems for nutraceuticals: A case study–Delivery by Design of curcumin. J Colloid Interface Sci 2019; 557: 506-518
- 13 Rapalli VK, Kaul V, Gorantla S. et al. UV Spectrophotometric method for characterization of curcumin loaded nanostructured lipid nanocarriers in simulated conditions: Method development, in-vitro and ex-vivo applications in topical delivery. Spectrochim Acta A Mol Biomol Spectrosc 2020; 224: 117392
- 14 Sharma D, Kaur P, Singh G. et al. Development and Validation of stability indicating UV-Visible spectrophotometric method for simultaneous estimation of neem (Azadirachtin) and curcumin in pharmaceutical tablet dosage form. Anal Chem Lett 2019; 9: 564-581
- 15 Sharma K, Agrawal SS, Gupta M. Development and validation of UV spectrophotometric method for the estimation of curcumin in bulk drug and pharmaceutical dosage forms. Int J Drug Dev Res 2012; 4: 375-380
- 16 Savale SK. UV spectrophotometric method development and validation for quantitative estimation of curcumin. Asian Journal of Biomaterial Research 2017; 3: 14-18
- 17 Kadam PV, Bhingare CL, Nikam RY. et al. Development and validation of UV spectrophotometric method for the estimation of curcumin in cream formulation. Pharm Methods 2013; 4: 43-45