Abstract
The inflammatory disease’s increased prevalence leads to a major concern around the
world. Still, there is a lack of effective and successful therapy in the reversal
of Inflammatory Bowel Disease (IBD) symptoms. Whereas, reactive oxygen species (ROS)
production and muddled defense capacity of antioxidants in IBD subjects reported several
times. Many proton pump inhibitors have been reported previously for their anti-inflammatory
effect. The present study is aimed to assess the ameliorative effect of lansoprazole
in experimentally induced IBD in rats. Thirty-six female Sprague Dawley rats were
divided equally into six groups based on their body weight. Lansoprazole (1, 5, and
10 mg/kg, p.o.) and 5-aminosalicylate (5-ASA, 100 mg/kg, p.o.) served as standard
control respectively, given for 18 days once a day. On the 11th day of the study,
colitis was induced by intrarectal instillation of 2, 4-Dinitrobenzene sulfonic acid
(DNBS), and treatment was continued for the next 7 days. Administration of lansoprazole
(at 5 and 10 mg/kg) significantly reduced DAI (Disease Activation Index) and CMDI
(Colon Macroscopic Damage Index); which further justifies a reduction in colon inflammation
grades, as well as histopathological changes, and reflected by the stalling of body
weight. The anti-inflammatory effects were indicated by lowered MPO (myeloperoxidase)
and SOD (superoxide dismutase) in colon tissue as well as restores colonic NO (nitric
oxide) level. The study shows lansoprazole improved DAI and CMDI scores, reduction
of neutrophil infiltration, and an improved antioxidant status indicating an anti-ulcerative
effect in DNBS-induced experimental colitis that is comparable with 5-ASA treatment.
Key words
IBD - DNBS - lansoprazole - myeloperoxidase - nitric oxide
