Endoscopy 2022; 54(06): E254-E255
DOI: 10.1055/a-1506-2683
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Pathological evaluation of a rectal endoscopic submucosal dissection specimen using micro-computed tomography

1   Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA
2   Department of Medicine, Division of Gastroenterology, Jichi Medical University, Shimotsuke, Japan
,
Makoto Nishimura
3   Gastroenterology, Hepatology and Nutrition Service, Memorial Sloan Kettering Cancer Center, New York, New York, USA
,
Alexei Teplov
1   Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA
,
Emine Cesmecioglu
1   Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA
,
Noboru Kawata
1   Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA
4   Division of Endoscopy, Shizuoka Cancer Center, Shizuoka, Japan
,
Jinru Shia
1   Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA
,
Yukako Yagi
1   Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, New York, USA
› Author Affiliations
Supported by: Nikon Metrology NV
Supported by: Warren Alpert Foundation, http://dx.doi.org/10.13039/100002558
Supported by: The Cancer Center Support Grant of the National Institutes of Health/National Cancer Institute P30CA008748

Endoscopic submucosal dissection (ESD) is widely used for resecting superficial gastrointestinal neoplasms that are difficult to remove en bloc by conventional endoscopic mucosal resection. After the resected specimen is sectioned into slices a few millimeters thick, slides are created for histologic examination. However, the slides produced represent only a small fraction of the lesion, and there is always the possibility of missing significant findings in the unexamined areas. Micro-computed tomography (micro-CT) is a novel in vitro tomographic method that allows the examination of fresh tissue or paraffin blocks non-destructively with a spatial resolution [1]. Previous studies have shown the potential of micro-CT images in revealing the detailed structures of breast cancer and thyroid cancer [2] [3]. Here we describe a superficial rectal cancer removed by ESD for which micro-CT revealed diagnostic information that was not well visualized on routine histology.

A 51-year-old woman was referred for resection of a laterally spreading tumor in the rectum. The patient underwent ESD and the lesion was removed en bloc. The resected specimen was immersed in 10 % Lugol’s iodine solution for 2 minutes and then scanned with a custom-made micro-CT for 10 minutes (Nikon Metrology NV, Leuven, Belgium) ([Fig. 1]). Thereafter, the specimen was processed following the standard procedure. There was no effect of immersion in iodine on the quality of the slides. Paraffin blocks were also subjected to micro-CT scanning after slide preparation. Micro-CT images clearly showed the submucosal invasion noted on the hematoxylin and eosin (H&E)-stained slide, especially in the block scan ([Fig. 2]). In addition, the micro-CT generated images between the sections that could not be seen with H&E staining ([Video 1]).

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Fig. 1 Appearance of the specimen resected by endoscopic submucosal dissection: a macroscopically, after penetration with 10 % Lugol’s iodine solution; b on micro-computed tomography imaging.
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Fig. 2 Images of the site of submucosal invasion (green arrows) on: a hematoxylin and eosin staining; b micro-computed tomography (micro-CT) of the fresh specimen; c micro-CT of the paraffin-embedded block.

Video 1 Micro-computed tomography for an endoscopic submucosal dissection specimen from an early rectal cancer.


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In conclusion, micro-CT scanning of fresh ESD specimens and paraffin blocks has the potential to enhance pathological diagnosis by providing a detailed histological structure of the areas that are not represented by standard H&E-stained slides.

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Publication History

Article published online:
08 June 2021

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