Planta Med 2023; 89(05): 526-538
DOI: 10.1055/a-1853-7101
Biological and Pharmacological Activity
Original Papers

Anti-adipogenic Effects of Sulforaphane-rich Ingredient with Broccoli Sprout and Mustard Seed in 3T3-L1 Preadipocytes

Xiao Men
1   Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea
,
Xionggao Han
1   Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea
,
Se-Jeong Lee
1   Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea
,
Keun-Tae Park
2   Research and Development Center, Milae Bioresourece Co. Ltd., Seoul, Korea
,
Jong-Kwon Han
2   Research and Development Center, Milae Bioresourece Co. Ltd., Seoul, Korea
,
Sun-Il Choi*
1   Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea
,
Ok-Hwan Lee*
1   Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon, Korea
› Author Affiliations
Supported by: 2020 Research Grant from Gangneung Science & Industry Promotion Agency
Supported by: the BK21 FOUR (Fostering Outstanding Universities for Research) funded by the Ministry of Education (MOE, Korea) and National Research Foundation of Korea (NRF) 4299990913942
Supported by: Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE, Korea) NRF-2017R1D1A3B0602846915
Supported by: Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education NRF-2021R1A6A1A03044242

Abstract

Glucoraphanin (GRA) is a precursor of sulforaphane (SFN), which can be synthesized by the enzyme myrosinase. In this study, we developed and validated HPLC analytical methods for the determination of GRA and SFN in mustard seed powder (MSP), broccoli sprout powder (BSP), and the MSP-BSP mixture powder (MBP), and evaluated their anti-adipogenic effects in 3T3-L1 adipocytes. We found that the analysis methods were suitable for the determination of GRA and SFN in MSP, BSP, and MBP. The content of GRA in BSP was 131.11 ± 1.84 µmol/g, and the content of SFN in MBP was 162.29 ± 1.24 µmol/g. In addition, BSP and MBP effectively decreased lipid accumulation content without any cytotoxicity. Both BSP and MBP significantly inhibited the expression of adipogenic proteins and increased the expression of proteins related to lipolysis and lipid metabolism. BSP and MBP inhibited the expression of adipocyte protein 2 (aP2), CCAAT/enhancer-binding protein-α (C/EBP-α), and peroxisome proliferator-activated receptor-γ (PPAR-γ) in 3T3-L1 adipocytes, and inhibited the expression of fatty acid synthase (FAS) through AMP-activated protein kinase (AMPK). Meanwhile, BSP and MBP also increased the expression of the lipolysis-related proteins, uncoupling protein-1 (UCP-1) and carnitine palmitoyltransferase-1 (CPT-1). Moreover, MBP exerted anti-adipogenic to a greater extent than BSP in 3T3-L1 preadipocytes.

* Co-correspondence authors.


Supporting Information



Publication History

Received: 13 January 2022

Accepted after revision: 16 May 2022

Accepted Manuscript online:
16 May 2022

Article published online:
11 October 2022

© 2022. Thieme. All rights reserved.

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Rüdigerstraße 14, 70469 Stuttgart, Germany

 
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