Abstract
Given the characters of “Silent killer”, epithelial ovarian
cancer (EOC) usually suffered late diagnosis and poor prognosis. Therefore, this
study aimed to explore the prognostic significance of ASMTL-AS1 in EOC and
investigated the effect of lncRNA ASMTL-AS1 dysregulation on tumor cellular
function. ASMTL-AS1 expression was analyzed in 133 EOC tissues and five kinds of
cell lines by RT-qPCR. The expression of ASMTL-AS1 was tested for correlation
with clinical data using the chi-square test and clinical follow-up using
Kaplan-Meier method with log-rank test. Further, the prognostic parameters in
predicting EOC overall survival were assessed by using multivariate Cox
proportional hazards analysis. In vitro assays, including MTT assay and
transwell assay, were conducted using EOC cell lines with overexpression of
ASMTL-AS1. In tumorous tissues and cell lines, ASMTL-AS1 was lowly expressed
compared with normal ones. This downregulation was associated with the advanced
FIGO stage, positive ascites cytology, and lymph node. In particular, low levels
of ASMTL-AS1 were revealed to have a high prognostic impact on EOC. ASMTL-AS1
overexpression strongly decreased cell proliferation, migration, and invasion in
vitro partly by moderating miR-1228-3p. This study demonstrates a significant
role for lowly expressed ASMTL-AS1 in EOC allowing for the prediction of
prognosis for EOC. Considering that ASMTL-AS1 is strongly involved in cell
growth and invasion, ASMTL-AS1 may be a promising marker for EOC prognosis and
therapy
Key words
LncRNA - ASMTL-AS1 - epithelial ovarian cancer - prognosis
