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Planta Med 2023; 89(03): 333-346
DOI: 10.1055/a-1942-5504
Pharmacokinetic Investigations
Original Papers

Metabolite Profiling of Swertia cincta Extract in Rats and Pharmacokinetics Study of Three Bioactive Compounds Using UHPLC-MS/MS

Xiaoyin Ye
2   School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
Tong Zhang
1   Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China
2   School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
Han Han 
1   Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China
2   School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
› InstitutsangabenGefördert durch: National Scientific and Technological Major Special Project of China 2019ZX09201004-002
Gefördert durch: Natural Science Foundation of Shanghai 21ZR1460500
Gefördert durch: National Natural Science Foundation of China 82173946
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Abstract

Swertia cincta, a plant of the genus Swertia in Gentianceae, has “heat-clearing” and detoxifying effects that normalize the gallbladder function in the treatment of jaundice. Although numerous studies on Swertia cincta have been performed, the absorption and pharmacokinetic behaviors remain unclear. In this study, the compounds of Swertia cincta in serum, bile, feces, and urine of rats were analyzed using a ultra-high-performance liquid chromatography-tandem mass spectrometry. A total of 9 prototype components and 48 metabolites were detected in biological samples. Furthermore, we determined the main components absorbed in the blood of Swertia cincta and established a method for simultaneously determining these components (sweroside, swertiamarin, and gentiopicroside) in positive ionization mode within 6 min. The quantitative method was successfully applied for the multiple-component pharmacokinetic study of Swertia cincta.

Key words

Swertia cincta - Gentianaceae - UHPLC-MS/MS - Pharmacokinetics - Metabolites

Supporting Information

    The supporting information includes 4 tables on method validation of pharmacokinetics experimental results and 8 figures on fragment ions of metabolites. Table 1S: Regression equation, correlation coefficients and linearity ranges for three analytes. Table 2S: Precision and accuracy of the three analytes in rat plasma. Table 3S: Recovery and matrix effect of the three analytes and IS in rat plasma. Table 4S: The stability of three analytes in rat plasma under different conditions. Fig. 1S: Fragment ion diagrams of metabolites of loganic acid. Fig. 2S: Fragment ion diagrams of metabolites of sweroside. Fig. 3S: Fragment ion diagrams of metabolites of swertiamarin. Fig. 4S: Fragment ion diagrams of metabolites of erythrocentaurin. Fig. 5S: Fragment ion diagrams of metabolites of isovitexin. Fig. 6S: Fragment ion diagrams of metabolites of isoorientin. Fig. 7S: Fragment ion diagrams of metabolites of maslinic acid. Fig. 8S: Fragment ion diagrams of metabolites of oleanolic acid.

  • Ergänzendes Material


Publikationsverlauf

Eingereicht: 16. Mai 2022

Angenommen nach Revision: 13. September 2022

Accepted Manuscript online:
13. September 2022

Artikel online veröffentlicht:
20. November 2022

© 2022. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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