A Neutral Glucan Extracted from Dried Ginger (Zingiberis Rhizoma): Preparation, Structure Characterization, and Immunomodulatory Activity

Long Sun; Xing Ni; Yulin Liu; Yantao Jiang; Pei-Pei Wang; Jingdong Gao

doi:10.1055/a-2574-2730

Planta Medica, Inhaltsverzeichnis

Planta Med 2025; 91(10/11): 558-568
DOI: 10.1055/a-2574-2730

Original Papers

A Neutral Glucan Extracted from Dried Ginger (Zingiberis Rhizoma): Preparation, Structure Characterization, and Immunomodulatory Activity

Autor*innen

Long Sun‡

²Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China
Xing Ni‡

¹Oncology Department, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, Jiangsu, China
Yulin Liu‡

²Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China
Yantao Jiang

¹Oncology Department, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, Jiangsu, China
Pei-Pei Wang

²Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China

³Marine Biomedical Science and Technology Innovation Platform of Lin-Gang Special Area, Shanghai, China
Jingdong Gao

¹Oncology Department, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, Jiangsu, China

⁴Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China

Abstract

A neutral glucan, GJ0D, was obtained from dried ginger (Zingiberis rhizoma) by enzymatic extraction and purification with column chromatography. The fine structure of GJ0D was assessed through monosaccharide composition analysis, methylation, and two-dimensional nuclear magnetic resonance. GJ0D has a relative molecular weight of 4.0 KDa and possesses a backbone consisting of 1,4-linked α-Glcp with substitution at C-6 of Glcp by T-Glcp. Immunoactivity assessment showed that GJ0D significantly upregulates the expression of IL-6, IL-1β, and TNF-α in RAW264.7 cells. The reactive oxygen species (ROS) production was also increased in RAW264.7 cells. In addition, the expression of several proteins associated with immune activation signaling pathways including TLR4, the phosphorylation of IKKβ, and NF-κB (p100 and p52) were significantly upregulated by GJ0D. These results suggest that GJ0D could promote inflammation through the TLR4/IKKβ/P100 signaling pathway, suggesting a potential application as an immunomodulating agent.

Keywords

ginger - Zingiber officinale - Zingiberaceae - structure characterization - immunomodulatory activity - glucan

Volltext

Referenzen

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