Establishment of a Carrot Cell Plant Growth Regulators-free System to Produce a Low-Cost Vaccine Candidate

Christian Carreño-Campos; Sahara Dubraiicka Elguea Zarate; Andrea Romero-Maldonado; María Luisa Villarreal; Sergio Rosales-Mendoza; Anabel Ortiz-Caltempa

doi:10.1055/a-2626-4703

Planta Medica, Table of Contents

Planta Med 2025; 91(12): 656-663
DOI: 10.1055/a-2626-4703

Original Papers

Establishment of a Carrot Cell Plant Growth Regulators-free System to Produce a Low-Cost Vaccine Candidate

Authors

Christian Carreño-Campos

¹Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México
Sahara Dubraiicka Elguea Zarate

¹Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México
Andrea Romero-Maldonado

²Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina (CICSaB), Universidad Autónoma de San Luis Potosí, San Luis Potosí (SLP), México
María Luisa Villarreal

¹Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México
Sergio Rosales-Mendoza

²Sección de Biotecnología, Centro de Investigación en Ciencias de la Salud y Biomedicina (CICSaB), Universidad Autónoma de San Luis Potosí, San Luis Potosí (SLP), México

³Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
Anabel Ortiz-Caltempa

¹Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México

Abstract

Parkinsonʼs disease is characterized by an abnormal accumulation of alpha synuclein (α-syn) in different regions of the central nervous system. At present, only palliative pharmacological treatments are available for Parkinson´s disease. Immunotherapy is considered an alternative to treat Parkinsonʼs disease, and plants are a convenient alternative platform for biopharmaceutical production. When compared to other systems, plants are particularly attractive because they offer cost-effectiveness, large-scale production, and enhanced safety. Therefore, this study aimed to establish a carrot cell suspension culture for the production of cLTB-Syn, a vaccine candidate against Parkinsonʼs disease. The convenience of MS medium optimization was demonstrated. Transgenic callus cultures were maintained and adapted on solid MSU9 medium without phytohormones, followed by growth kinetics in suspension cultures. The maximum biomass yield was 15.8 ± 0.35 g/L DW at 14 days of culture, with a growth rate of µ = 0.1034/d and td = 6.7 days. The cLTB-Syn protein production reached a maximum value of 2.62 ± 0.03 µg/g DW, representing a 1.6-fold increase over the initial culture time. Finally, the presence of the transgene was confirmed by PCR, and the integrity of cLTB-Syn protein was determined by dot blot assays. This study presents evidence of a promising system for a toxin-free biopharmaceutical production, which has the potential to be scaled up for large manufacturing, at a low cost.

Keywords

Daucus carota - Apiaceae - Parkinsonʼs disease - toxin-free biopharmaceutical - callus culture

Full Text

References

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