Protective Effect of Boldine Against Diclofenac-Induced Renal Damage
                    in Rats

Luísa Nathália Bolda Mariano; Heloísa Roberti Cristofolini; Rita de Cássia Vilhena da Silva; Anelise Felício Macarini; Valdir Cechinel Filho; Priscila de Souza

doi:10.1055/a-2749-3102

Drug Research, Table of Contents

Drug Res (Stuttg)
DOI: 10.1055/a-2749-3102

Original Article

Protective Effect of Boldine Against Diclofenac-Induced Renal Damage in Rats

Boldine Shields Kidneys from Diclofenac

Authors

Luísa Nathália Bolda Mariano

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brazil (Ringgold ID: RIN37875)
Heloísa Roberti Cristofolini

²Medical course, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brazil (Ringgold ID: RIN37875)
Rita de Cássia Vilhena da Silva

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brazil (Ringgold ID: RIN37875)
Anelise Felício Macarini

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brazil (Ringgold ID: RIN37875)
Valdir Cechinel Filho

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brazil (Ringgold ID: RIN37875)
Priscila de Souza

¹Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Brazil (Ringgold ID: RIN37875)

Abstract

Objective

This study investigated the protective effect of alkaloid boldine against diclofenac-induced kidney damage in normotensive female rats.

Methods

Animals were divided into three groups: naive, vehicle+diclofenac (50 mg/kg), and boldine+diclofenac (boldine 0.1 mg/kg+diclofenac 50 mg/kg). Treatments were administered orally once daily for 2 days.

Results

The vehicle+diclofenac group showed reduced urinary volume and sodium excretion. In contrast, the boldine+diclofenac group restored both parameters to levels similar to the naive group. Other urinary electrolytes indicated imbalance in diclofenac-treated animals, regardless of boldine co-treatment. Plasma analysis showed no alterations. Kidney tissue from diclofenac-treated groups revealed increased glutathione and decreased lipid hydroperoxides. Histology showed that vehicle+diclofenac resulted in a reduction in glomerular size, thickening of Bowman’s capsule, and mesangial disarray, while these changes were less pronounced with boldine co-treatment. Molecular docking analysis indicated that boldine may interact with important proteins related to renal hemodynamics, sodium regulation, and inflammatory processes pointing to a multi-target mechanism.

Conclusions

Boldine attenuated renal damage induced by diclofenac, improving urinary parameters and reducing histological alterations. Further studies are necessary to elucidate its protective mechanisms and impact on renal hemodynamics.

Keywords

non-steroidal anti-inflammatory - electrolytes - kidney - oxidative stress

Full Text

References

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