Analysis of the volatile constituents of Cornus mas (Cornaceae) fruits

P. Tsatalas; M. Spanakis; E. Kokkalou

doi:10.1055/s-0028-1084946

Planta Medica, Table of Contents

Analysis of the volatile constituents of Cornus mas (Cornaceae) fruits

P Tsatalas ¹, M Spanakis ², E Kokkalou ¹

¹Laboratory of Pharmacognosy, Department of Pharmacognocy-Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece
²Department of Pharmacognosy-Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece

Abstract

There have been no previous references about volatile compounds in Cornus mas fruits. The aim of this study is important since Cornus mas fruits are widely used for the preparation of beverages, liqueurs and distillates. 1kg of fruits was subjected to steam distillation according to the procedure suggested by the Third European Pharmacopoeia. The attribution of the volatiles was 1‰ v/w (0.001ml) and the fraction was collected in pentane.

The analysis was carried out in a Shimadzu GC 2010 gas chromatographer. Split type sample introduction, column HP-5 MS (30m x 0.25mm, 0.25µm), injection temperature 240^oC, [50^oC(1min), (4^oC/min)→220^oC (5min)].

The GC-MS analysis was carried out in a GC/MS QP 2010 mass analyzer (70 eV, ion source temp. 220^oC).

The existence of at least sixty two constituents in the whole fraction was confirmed. Up to now, about the 80% of the existing compounds was identified [monoterpenic compounds (14.02%), sesquiterpenic compounds (11.48%), diterpenes (2.18%), aliphatic derivatives (31.74%), aromatic derivatives (7.21%), furan derivatives (10.06%), cyclohexane derivatives (3.31%)] [1–5].

References: 1. Jennings N., Shibamoto T. (1980) Quantitative Analysis of Flavor and Fragrance Volatiles by G. Capillary Gas Chromatography. Academic Press. N. York.

2. Adams R.P. (1989) Identification of Essential Oils by Ion Trap Mass Spectroscopy. Academic Press. N. York.

3. Adams R.P. (1995) Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. Allured Publishing Corporation. Illinois USA.

4. Adams R.P. (2007) Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry. Allured Publishing Corporation. Carol Stream.

5. Spectral Library Nist.