Horm Metab Res 2009; 41(1): 62-66
DOI: 10.1055/s-0028-1087172
Humans, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Modulation of Insulin Concentrations and Metabolic Parameters in Obese Patients by −55CT Polymorphism of the UCP3 Gene Secondary to Two Hypocaloric Diets

D. A. de Luis 1 , R. Aller 1 , O. Izaola 1 , M. Gonzalez Sagrado 1 , R. Conde 1
  • 1Institute of Endocrinology and Nutrition, Medicine School and Unit of Investigation, Hospital Rio Hortega, University of Valladolid, Valladolid, Spain
Weitere Informationen

Publikationsverlauf

received 25.02.2008

accepted after second revision 15.07.2008

Publikationsdatum:
29. September 2008 (online)

Abstract

Decreased expression or function of UCP3 could reduce energy expenditure and increase the storage of energy. The aim of our study was to investigate the influence of −55CT polymorphism of the UCP3 gene on insulin concentrations and metabolic parameters secondary to two hypocaloric diets in obese patients. A population of 131 obese (body mass index >30), nondiabetic outpatients was analyzed in a prospective way. Before and after 2 months on two different hypocaloric diets, bipolar electrical bioimpedance, blood pressure measurement, serial assessment of nutritional intake with 3 d of written food records, and biochemical analysis were performed. The statistical analysis was performed based on a dominant model. With diet type I (low fat) and diet type II (low carbohydrate) in probands with both wild-type alleles, we observed decreases in BMI, weight, fat mass, systolic blood pressure, leptin levels, and insulin concentrations. In addition, with diet type II, a decrease in diastolic blood pressure, total cholesterol, and triglyceride levels was detected. Secondary to diet type I, a decrease in waist circumference and TNF-alpha was observed. Carriers of the T variant experienced decreases in BMI, weight, and fat mass on both diets, without statistical changes in biochemical parameters. In probands with both C alleles, both diets decreased insulin concentrations, blood pressure, and leptin concentrations. Weight reduction was similar with C and T alleles, independent of macronutrient distribution.

References

  • 1 Aranceta J, Perez Rodrigo C, Serra Majem L. Prevalencia de la obesidad en España: estudio SEEDO 97.  Med Clin (Barc). 1998;  111 441-445
  • 2 Tuomilehto J, Lindstrom J, Eriksson JG. Finnish Diabetes Prevention Study Group: prevention of type 2 diabetes mellitus changes in lifestyle among subjects with impaired glucose tolerance.  N Engl J Med. 2001;  344 1343-1350
  • 3 Matsuzawa Y. Adipocytokines: Emerging therapeutic targets.  Current Atherosclerosis Reports. 2005;  7 58-62
  • 4 Vidal Puig A, Solanes G, Grujic D, Flier J, Lowell BB. UCP3: an uncoupling protein homologue expressed preferentially and abundantly in skeletal muscle and brown adipose tissue.  Biochem Biophys Res Commun. 1997;  235 79-82
  • 5 Saltzman E, Roberts SB. The role of energy expenditure in energy regulation: findings from a decade research.  Nutr Rev. 1995;  53 209-220
  • 6 Bouchard C, Perusse L, Chagnon YC, Warden C, Ricquier D. Linkage between markers in the vicinity of uncopuling protein 2 gene and resisting metabolic rate in humans.  Hum Mol Genet. 1997;  6 1887-1889
  • 7 Clapham J, Arch J, Chapman H. Mice overexpressing human uncoupling protein 3 in skeletal muscle are hyperphagic lean.  Nature. 2000;  406 415-418
  • 8 Samec S, Seydoux J, Dullo A. Role of UCP homologues in skeletal muscle and free fatty acids in obesity.  Lancet. 1998;  351 1933-1934
  • 9 Schrauwen P, Xia Xia J, Walder K, Snitker S, Ravussin E. A novel polymorphism in the proximal UCP3 promoter region: effect on skeletal muscle. UCP3 m RNA expression and obesity in male nondiabetic Pima Indians.  Int J Obes. 1999;  23 1242-1245
  • 10 Otabe S, Clement K, Dina C. A genetic variation in the 5′ flanking region of the UCP3 is associated with body mass index in humans in interaction with physical activity.  Diabetologia. 2000;  43 245-249
  • 11 Meirhaeghe A, Amoyel P, Helbecque N. An uncoupling protein 3 gene polymorphism associated with a lower risk of developing type 2 diabetes and with atherogenic lipid profile in a French cohort.  Diabetologia. 2000;  43 1424-1428
  • 12 Mathews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF. Homeostasis model assessment: insulin resistance and beta cell function from fasting plasma glucose and insulin concentrations in man.  Diabetologia. 1985;  28 412-414
  • 13 Feurer ID, Mullen JL. Bedside measurement of resting energy expenditure and respiratory quotient via indirect calorimetry.  Nutr Clin Pract. 1986;  1 43-49
  • 14 Pichard C, Slosman D, Hirschel B, Kyle U. Bioimpedance analysis in AIDS patients: an improved method for nutritional follow up.  Clin Res. 1993;  41 53
  • 15 Mataix J, Mañas M. Tablas de composición de alimentos españoles. Ed: University of Granada 1998
  • 16 Hesselink MK, Mensink M, Schrauwen P. Human uncoupling protein-3 and obesity: an update.  Obes Res. 2003;  11 1429-1443
  • 17 Millet L, Vidal H, Andreelli F. Increased uncoupling protein 2 and 3 mRNA expression during fasting in obese and lean humans.  J Clin Invest. 1997;  100 2665-2670
  • 18 El bein SC, Lepperrt M, Hasstedt S. Uncoupling protein 2 region on chromosome 11q13 is not linked to markers of obesity in familial type 2 diabetes.  Diabetes. 1997;  46 2105-2107
  • 19 Dalgaard LT, Sorensen TIA, Drivshom T, Borch Johnsen K, Andersen T, Hansen T. A prevalent polymorphism in the promoter of the UCP3 gene and its relationship to body mass index and long term body weight change in the Danish population.  J Clin Endoc Metab. 2001;  86 1398-1402
  • 20 Alonso A, Marti A, Corbalan MS, Martinez Gonzalez MA, Forga L, Martinez JA. Association of UCP3 gene −55CT polymorphism and obesity in a Spanish population.  Ann Nutr Metab. 2005;  49 183-188
  • 21 Liu YJ, Liu PY, Long J, Lu Y, Elze L, Recker RR, Deng HW. Linkage and association analyses of the UCP3 gene with obesity phenotypes in Caucasian families.  Physiol Genom. 2005;  22 197-203
  • 22 Cassell PG, Saker PJ, Huxtable SJ, Kousta E, Jackson AE, Hattersle AT. Evidence that single nucleotide polymorphism in the uncoupling protein 3 gene influences fat distribution in women of European and Asian origin.  Diabetologia. 2000;  43 1558-1564
  • 23 Commins SP, Watson PM, Framton IC, Gettys TW. Leptin selectively reduces white adipose tissue in mice a UCO1 dependent mechanism in BAT.  J Physionl Endocrinol Metan. 2005;  280 372-377
  • 24 Frayn KN, Karpe F, Fielding BA, Macdonald IA, Coppack SW. Integrative physiology of human adipose tissue.  Int J Obes Relat Metab Disord. 2003;  27 875-888
  • 25 Xenachis C, Samojlik E, Raghuwanshi MP, Kirschner MA. Leptin, insulin and TNF-alpha in weight loss.  J Endocrinol Invest. 2001;  24 865-870
  • 26 Bastard JP, Jardel C, Bruckert E. Elevated levels of interleukin-6 are reduced in serum and subcutaneous adipose tissue of obese women after weight loss.  J Clin Endocrinol Metab. 2000;  85 3338-3342
  • 27 Monzillo LU, Hamdy O, Horton ES, Ledbury S, Mulloly C, Jarema C, Porter S, Ovalle K. Efect of lifestyle modification on adipokine levels in obese subjects with insulin resistance.  Obesity Res. 2003;  11 1048-1052
  • 28 Luis DA de, Aller R, Izaola O, Gonzalez Sagrado M, Conde R. Modulation of adipocytokynes response and weight loss to a hypocaloric in obese patients by −55ct polymorphism of ucp3 gene.  Horm Metab Res. 2008;  40 241-218
  • 29 Shen H, Xiang K, Jia W. Effects of uncoupling protein 3 gene −55 CT variant on lipid metabolism, body fat, its distribution and noninsulin dependent diabetes mellitus in Chinese.  Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002;  19 317-321
  • 30 Yudkin JS. Inflammation, obesity and the metabolic sindrome.  Horm Metab Res. 2007;  39 707-709
  • 31 Luis DA de, Aller R, Izaola O, Gonzalez Sagrado M, Conde R, Perez Castrillon JL. Lack of association of -55CT polymorphism of UCP3 gene with fat distribution in obese patients.  Ann Nutr Metab. 2007;  51 374-378
  • 32 Luis DA de, Sagrado MG, Aller R, Izaola O, Conde R. Influence of ALA54Thr polymorphism of fatty acid binding protein 2 on obesity and cardiovascular risk factors.  Horm Metab Res. 2007;  39 830-834

Correspondence

Dr. D. A. de Luis

Professor Associated of Nutrition

Executive Director

Institute of Endocrinology and Nutrition

Medicine School

Valladolid University

C/Los Perales 16

Simancas 47130

Valladolid

Spain

Telefon: +34/9834/20 40 0

Fax: +34/9833/31 56 6

eMail: dadluis@yahoo.es

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