Monolayer cultures derived from neonatal hamster or rat pancreas by two different
epithelioid cell-enriching gravity sedimentation procedures varied in ability to maintain
uniform levels of insulin secretion with increased culture age. Rat pancreatic cultures
were superior in this respect to identically derived hamster preparations, depending
on the preparative procedure employed. Quantitative differences in the temporal pattern
of insulin secretion by different rat pancreatic culture preparations were ascribed
to plating cell density and consequent terminal cell density as a function of preparative
procedure such that reduced densities favored sustained secretory levels. These findings
suggest the importance of tissue species and preparative procedure in deriving pancreatic
monolayer cultures capable of sustained levels of insulin secretion with age.
Pancreatic Monolayer Cultures, Hamster, Rat - Secretion, Insulin, Glucagon - Culture
Cell Density-Dependent Inhibition
