Abstract
The development of obesity, hyperinsulinemia and six hepatic lipogenic enzymes in
Avy/a mice were compared to that in a/a mice. Correlation between body weight, liver
weight, plasma insulin concentration and activities of hepatic enzymes was analyzed.
In the Avy/a mice, body weight, liver weight and plasma insulin level increased steadily as
the mice aged. In the a/a mice, the change of these three parameters was much slower.
Plasma insulin concentration in a/a mice did not increase until eight months of age.
Compared with a/a mice, Avy/a mice had higher 6-phosphogluconate dehydrogenase and fatty acid synthetase activities
at two months of age; lower citrate cleavage enzyme, glucose-6-phosphate dehydrogenase
and 6-phosphogluconate dehydrogenase activities at three months of age; lower citrate
cleavage enzyme and glucose-6-phosphate dehydrogenase and higher acetyl CoA carboxylase
activities at five months of age; and higher malic enzyme, citrate cleavage enzyme
and 6-phosphogluconate dehydrogenase activities at eight months of age.
There were significant correlations between plasma insulin level and body weight
and between plasma insulin level and the activities of malic enzyme and citrate cleavage
enzyme in Avy/a mice. The correlation between body weight and malic enzyme and citrate cleavage
enzyme activities disappeared after the analysis was adjusted for plasma insulin level.
ACC = Acetyl CoA Carboxylase
CCE = Citrate Cleavage Enzyme
FAS = Fatty Acid Synthetase
G6PDH = Glucose-6-Phosphate Dehydrogenase
6PGDH = 6-Phosphogluconate Dehydrogenase
ME = Malic Enzyme
Key words
Obesity - Plasma Insulin - Hyperinsulinism - Lipogenic Enzymes - Malic Enzyme - Glucose-6-Phosphate
Dehydrogenase - 6-Phosphogluconate Dehydrogenase - Citrate Cleavage Enzyme - Acetyl
CoA Carboxylase - Fatty Acid Synthetase - Viable Yellow Obese Mice - Yellow Obese
Mice - Genetic Obesity
1 The work of PLY was supported in part by USPHS grants GM 21054 and DE 119.
