Lipolytic effects produced by permissive doses (0.025-1.0 µg/ml) of sheep β-lipotropin
(βLPH), were studied in adipose tissue and isolated cells of the rat. Preincubation
of tissue segments with βLPH for three hours augmented the glycerol release due to
theophylline subsequently added in fresh medium. In isolated fat cells, incubation
of βLPH, together with dihydroergotamine (DHE) and dexamethasone (DEX), stimulated
the release of FFA to a level which exceeded that produced by any other combination
of agents in the experiment. This effect was suppressed by cycloheximide. The effects
described for βLPH are similar to those produced by growth hormone preparations. The
data presented suggests that the stimulation of lipolysis by βLPH in the rat may be
a function of protein synthesis.
Lipolysis - Rat Adipose Tissue and Cells - Sheep βLipotropin - Human Growth Hormone
- Theophylline - Dihydroergotamine - Dexamethasone
