The pathway and time course of the intracellular transport of new synthesised proteins
in the guinea pig pancreatic A cell has been determined by electron microscopic radioautography.
Protein biosynthesis occurs in the elements of the rough surfaced endoplasmic reticulum,
the newly synthesised proteins being transferred via transitional areas to the Golgi
complex where they may be present from 20-50 minutes after labelling. Storage granules
containing newly synthesised proteins are found at 50 or 120 minutes after the end
of pulse-labelling. The cellular mechanisms for the biosynthesis, intracellular transport
and storage of proteins in the A cell apparently closely resemble those previously
described in the B cells of rats and guinea pigs.
Protein Synthesis - Pancreatic A Cell - Guinea Pig - Intracellular Transport
