Abstract
The effect of chronic diabetes on the ability of soluble factors to support ribosomal
protein synthesis was studied. It was found that the hepatic supernatant fraction
prepared from pancreatectomized diabetic rats was less efficient than normal in catalyzing
the incorporation of 3H-phenylalanine into microsomal protein. The ability to incorporate radioactivity
from aminoacyl-sRNA into ribosomal protein, however, was unchanged in diabetes. Subsequent
experiments confirmed the impression that the formation of aminoacyl-sRNA is depressed
in the hepatic 30,000 rpm supernatant prepared from diabetic animals.
Key words
Diabetes - Protein Synthesis - Soluble Enzyme Activity
1 Present Address: Dr. Margo P. Cohen, Wayne State University School of Medicine, Department
of Medicine,1400 Chrysler Freeway, Detroit, Michigan 48207, USA
