A reliable method for the production of ACTH-antibodies with high titres is described.
ACTH was conjugated by means of ethyl-carbodiimide. Guinea pigs and rabbits were immunized.
The sensitivity of the resulting antisera was evaluated by plotting a standard-curve
with each antiserum. With the best antisera 1 pg ACTH/ml could be measured. Specificity
of the antisera was tested by setting up antiserum dilution curves with labelled αp
1-39 ACTH, βh 1-39 ACTH and β1-24 ACTH. Surprisingly, most of the antisera appeared to react specifically with the
N-terminal portion of the ACTH-chain.
Antibodies to ACTH - Sensitivity of Antibodies - Specificity of Antibodies - Radioimmunoassay
of ACTH
