A Rapid Filter Paper Disk Assay for Picomole Amounts of Cyclic AMP Using a Cyclic AMP Dependent Protein Kinase

F. R. Butcher

doi:10.1055/s-0028-1094138

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 1971; 3(5): 336-340
DOI: 10.1055/s-0028-1094138

Originals

A Rapid Filter Paper Disk Assay for Picomole Amounts of Cyclic AMP Using a Cyclic AMP Dependent Protein Kinase[*]

F. R. Butcher [**]

University of Wisconsin, McArdle Laboratory for Cancer Research, Madison, Wisconsin, USA

Abstract

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Abstract

A rapid filter paper disk assay for cyclic AMP based on the activation of a cyclic AMP dependent protein kinase from rabbit skeletal muscle is described. This procedure permits determination of as little as 0.2 picomoles of cyclic AMP. In addition this procedure employs ATP-γ-³³P instead of ATP- γ-³²P.

Key words

Cyclic AMP - Cyclic AMP Assay - Protein Kinase

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Referenzen

1 This work was performed while the author was a USPHS postdoctoral fellow in the laboratory of Dr. Van R. Potter. Financial support was provided by departmental Grant CA 07175, Training Grant T01-Ca5002, and a USPHS postdoctoral fellowship 5 F02-CA-43,880-02 through the National Cancer Institute.

2 Address after July 1: Brown University; Division of Biological and Medical Sciences; Providence, Rhode Island 02912