Summary
To determine the protective effects of different methods of cardioplegia, studies
on ATP/lactate levels and ultrastructure were performed in human papillary muscles
obtained during mitral valve replacement. In group I (n = 5), plain ischemic arrest
in hypothermia (systemic venous temperature = 24 °C) was accomplished. In group II
(n = 12), the heart was arrested by injection cardioplegia using magnesium-aspartate-
procaine at systemic venous and myocardial temperatures of 24 °C. In group III (n
= 12) Bretschneider infusion cardio plegia at systemic venous and myocardial temperatures
of 26 °C and 19 °C respectively was applied.
With regard to ultrastructural changes there were no clear- cut differences in
the three methods of hypothermic cardiac arrest after 60 minutes of ischemia. Ischemic
changes tended to be slightest in group III (infusion cardioplegia). ATP decay and
lactate increase were significant in group I and moderate to minimal in groups II
and HI after the same period of time. It is concluded that for aortic cross- clamp
times up to 60 minutes, body hypothermia and injection cardioplegia using magnesium-aspartate-procaine
at a myocardial temperature of 24 °C provide adequate protection of the myocardium.
For ischemia times beyond 70 minutes, profound myocardial hypothermia below 20
°C is preferred.
Key-Words:
Myocardial protection - Myocardial metabolism - Myocardial ultrastructure - Myocardial
hypothermia - Cardioplegia
