Planta Med 2009; 75(5): 488-493
DOI: 10.1055/s-0029-1185316
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

The Flavonoid Rutin but not the Alkaloid Arborinine Induces Apoptosis in a B-Cell Hybridoma Cell Line

Renata Roseghini1 , 2 , Georgenes Morato Falcão1 , José Fernando Oliveira Costa3 , Jorge Clarêncio3 , Ivana Nascimento1 , Eudes Velozo4 , Robert Schaer1 , Vera Vale1 , Silvia Lima Costa5 , Maria de Fátima Dias Costa5 , Marcienne Tardy6 , Roberto Meyer1 , Songelí Menezes Freire1 , 2
  • 1Immunology and Molecular Laboratory (Labimuno), Federal University of Bahia, BA, Brazil
  • 2Escola Bahiana de Medicina e Saúde Pública (EBMSP), BA, Brazil
  • 3Oswaldo Cruz Foundation (CPqGM – FIOCRUZ), BA, Brazil
  • 4LAPEMM, Pharmacy Faculty-Federal University of Bahia, BA, Brazil
  • 5Laboratory of Neurochemical and Cellular Biology, Federal University of Bahia, BA, Brazil
  • 6Inserm, Université de Paris XII, Paris, France
Further Information

Publication History

received April 6, 2008 revised Dec. 1, 2008

accepted Dec. 7, 2008

Publication Date:
20 February 2009 (online)

Abstract

The effects of arborinine, an alkaloid extracted from Erthela bahiensis and of rutin, a flavonoid obtained from Dimorphandra mollis (Benth.), Brazilian medicinal plants, on the viability and function of a murine B-cell hybridoma as a tumor model were investigated. The flavonoid rutin at 50 µΜ induced an increase in the number of apoptotic cells of one- to fivefold and reductions in cellular proliferation and monoclonal antibody production. Less but still significant necrosis was also induced by rutin under the same experimental conditions. On the other hand, the alkaloid arborinine exerted no significant effects on the studied parameters.

References

  • 1 Reis M S, Mariot A. Diversidade natural e aspectos agronômicos de plantas medicinais. Simões CMO, editor Farmacognosia: da planta ao medicamento. Porto Alegre; Florianópolis, Universidade 1999: 39-41
  • 2 Ng T B, Liu F, Wang Z T. Antioxidative activity of natural products from plants.  Life Sci. 2000;  66 709-723
  • 3 Huang Y, Fang Y, Wu J, Dziadyk J M, Zhu X, Sui M, Fan W. Regulation of Vinca alkaloid-induced apoptosis by NF-kappaB/IkappaB pathway in human tumor cells.  Mol Cancer Ther. 2004;  3 271-277
  • 4 Geetha V S, Viswanathan S, Kameswaran L. Comparison of total alkaloids of Thylophora indica and disodium cromoglycate on mast cell stabilization.  Indian J Pharmacol. 1981;  13 199-201
  • 5 Chen J J, Deady L W, Mackay M F. Synthesis of some acridone alkaloids and related compounds.  Tetrahedron. 1997;  53 12717-12728
  • 6 Queener S F, Fujioka H, Nishiyama Y, Furukawa H, Bartlett M S, Smith J W. In vitro activities of acridone alkaloids against Pneumocystis carinii.  Antimicrob Agents Chemother. 1991;  35 377-379
  • 7 Kawaii S, Tomono Y, Katase E, Ogawa K, Yano M, Takemura Y, Ju-ichi M, Ito C, Furakawa H. Acridones as inducers of HL‐60 cell differentiation.  Leuk Res. 1999;  23 263-269
  • 8 Martinez-Florez S, Gonzallez-Gallego J, Culebras J M, Tuñon M J. Los flavonóides: propiedades y acciones antioxidantes.  Nutr Hosp. 2002;  XVII‐6 271-278
  • 9 Lorenzi H. Árvores Brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. Rio de Janeiro; Plantarum 1949: 175
  • 10 Janbaz K H, Saeed S A, Gilani A H. Protective effect of rutin on paracetamol- and CCl4-induced hepatotoxicity in rodents.  Fitoterapia. 2002;  73 557-563
  • 11 Deschner E E, Ruperto J, Wong G, Newmark H L. Quercetin and rutin as inhibitors of azoxymethanol-induced colonic neoplasia.  Carcinogenesis. 1991;  12 1193-1196
  • 12 Knowles L M, Zigrossi D A, Tauber R A, Hightower C, Milner J A. Flavonoids suppress androgen-independent human prostate tumor proliferation.  Nutr Cancer. 2000;  38 116-122
  • 13 Ostrakhovitch E A, Afanas'ev I B. Oxidative stress in rheumathoid arthritis leukocytes: suppression by rutin and other antioxidants and chelators.  Biochem Pharmacol. 2001;  62 743-746
  • 14 Roseghini R, Rocha D S, Clarêncio J, Costa S L, Costa M FD, Tardy M, Nascimento I, Schaer R, Velozo E, Meyer R, Freire S M. Flavonoid rutin alters the viability and function of mitogen stimulated splenocytes and thymocytes compared to non-stimulated cells.  Immunopharmacol Immunotoxicol. 2007;  29 271-285
  • 15 Roseghini R, Morrerra P RS, Vale V, Pinheiro A M, Costa J, Freire S M, Nascimento I, Schaer R, Velozo E, El-Bachá R, Meyer R. Different effects of arborinine alkaloid obtained from Brazilian Erthela baihensis on spleen and thymus cells stimulated in vitro with different mitogens.  Immunopharmacol Immunotoxicol. 2006;  28 361-376
  • 16 Alkan S. Monoclonal antibodies: the story of a discovery that revolutionized science and medicine.  Nat Rev Immunol. 2004;  4 153-156
  • 17 Santos C S, Januário A H, Vieira P C, Fernandes J B, Silva M FGF, Pirani J R. Cycloartane triterpenoid and alkaloids from Ameidea spp.  J Braz Chem Soc. 1998;  1 39-42
  • 18 Vale V LC, Costa M, Pacheco A, Carminati R, Gomes A P, Regis L F, Barreto L M, Oliveira S C, Meyer R. Evaluation of the immune response cellular profile from mice immunized with Corynebacterium pseudotuberculosis and stimulated by somatic and secreted antigen. São Pedro, SP, Brazil: Annals of XXX Meeting of the Brazilian Society of Immunology 2005: 240
  • 19 Petit P X, Lecouer H, Zorn E, Dauguet C, Mingott B. Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis.  J Cell Biol. 1995;  130 157-167
  • 20 Middleton E JR, Kandaswami C, Theoharides T C. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease and cancer.  Pharmacol Rev. 2000;  52 673-751
  • 21 Cerqueira F, Cordeiro-da-Silva A, Araújo N, Cidade H, Kijoa A, Nascimento M SJ. Inhibition of lymphocyte proliferation by prenylated flavones: artelastin as a potent inhibitor.  Life Sci. 2003;  73 2321-2334
  • 22 Quader M A, Nutan M TH, Rashid M A. Antitumor alkaloid from Glycosmis pentaphylla.  Fitoterapia. 1999;  70 305-307
  • 23 Hirano R, Sasamoto W, Matsumoto A, Itakura H, Igarashi O Y, Kondo K. Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation.  J Nutr Sci Vitaminol. 2001;  47 357-362
  • 24 Wei Y-Q, Zhao X, Kariya Y, Fukata H, Teshigawara K, Uchida A. Induction of apoptosis by quercetin: involvement of heat shock protein.  Cancer Res. 1994;  54 4952-4957
  • 25 Hanashiro K, Sunagawa M, Tokeshi Y, Nakasone T, Ohta S, Nakamura M, Kosugi T. Antiallergic drugs, azelastine hydrochloride and epinastine hydrochloride, inhibit ongoing IgE secretion of rat IgE-producing hybridoma FE‐3 cells.  Eur J Pharmacol. 2006;  547 174-183
  • 26 Meliksetian M B, Davtian T K, Chirkowa I V, Alexanian Y T, Ignatova T N. Alterations in immunoglobulin syntesis during adriamycin-induced apoptosis in mouse hybridoma cells.  Cell Biol Int. 1997;  21 69-74
  • 27 Vomastek T, Franěk F. Kinetics of development of spontaneous apoptosis in B cell hybridoma cultures.  Immunol Lett. 1993;  35 19-24
  • 28 Ündeğ Ü, Aydın S, Başaran A A, Başaran N. The modulating effects of quercetin and rutin on the mitomycin C induced DNA damage.  Toxicol Lett. 2004;  151 143-149
  • 29 Yinjun L, Jie J, Weilai X, Xiangming T. Homoharringtonine mediates myeloid cell apoptosis via upregulation of pro-apoptotic bax and inducing caspase-3mediated cleavage of poly (ADP-ribose) polymerase (PARP).  Am J Hematol. 2004;  76 199-204
  • 30 Solis-Maldonado C, Quintanilla-Licea R, Tamez-Guerra R, Rodríguez-Padilla C, Gomez-Flores R. Differential effects of synthetic indoloquinolisines on in vitro rat lymphocyte and macrofage functions.  Int Immunopharmacol. 2003;  3 1261-1271

Prof. Renata Roseghini

Instituto de Ciências da Saúde

Av. Reitor Miguel Calmon S/N

Vale do Canela

CEP: 40

110–100 Salvador

Bahia

Brazil

Phone: + 55 71 33 69 02 08

Fax: + 55 71 33 69 42 34

Email: bino7@terra.com.br

    >