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Int J Sports Med 2009; 30(7): 545-549
DOI: 10.1055/s-0029-1202341
Nutrition

© Georg Thieme Verlag KG Stuttgart · New York

Glycogen Synthesis after Road Cycling in the Fed State

A. Reinert 1 , D. Slivka 1 , J. Cuddy 1 , B. Ruby 1
  • 1Montana Center for Work Physiology and Exercise Metabolism, The University of Montana, Missoula, United States
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Publication History

accepted after revision January 10, 2009

Publication Date:
19 May 2009 (online)

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Abstract

The purpose of this study was to determine the effects of a recovery beverage immediately after exercise on rates of muscle glycogen resynthesis in response to road cycling when nutritional supplementation was supplied during exercise and a solid meal was served two hours after exercise. Eight trained male cyclists, (25±4 years, 69.3±5.2 kg, VO2 peak=4.5±0.4 L·min−1) performed two 62 km outdoor training rides in a double-blind, randomized cross-over experiment. Subjects received a food bar and a commercial sport drink during each ride. A recovery beverage (40 g CHO+20 g PRO) or a placebo (PL) was administered 30 min post-exercise. At 2 h post-exercise, a solid meal was provided for both trials. There was no difference between trials at any time point for glycogen (140±9, 56±8, and 70±8 mmol · kg−1 wet wt.−1 · hr.−1 for pre, post, and 4 h post, respectively). The addition of a supplemental recovery beverage ingested soon after exercise did not significantly increase the rate of muscle glycogen resynthesis after 4 h of recovery when nutritional supplementation is provided during exercise and a meal is consumed 2 h after exercise.

Key words

sport nutrition - carbohydrate - insulin - glucose - powertap - field study

References

  • 1 Arkinstall MJ, Tunstall RJ, Cameron-Smith D, Hawley JA. Regulation of metabolic genes in human skeletal muscle by short-term exercise and diet manipulation.  Am J Physiol. 2004;  287 E25-E31
    PubMedGoogle Scholar
  • 2 Bergstrom J, Hermansen L, Hultman E, Saltin B. Diet, muscle glycogen and physical performance.  Acta Physiol Scand. 1967;  71 140-150
    CrossrefPubMedGoogle Scholar
  • 3 Bertucci W, Duc S, Villerius V, Pernin JN, Grappe F. Validity and reliability of the Powertap mobile cycling powermeter when compared with the SRM device.  Int J Sports Med. 2005;  26 886-873
    Article in Thieme ConnectPubMedGoogle Scholar
  • 4 Blom PC, Hostmark AT, Vaage O, Kardel KR, Maehlum S. Effect of different post-exercise sugar diets on the rate of muscle glycogen synthesis.  Med Sci Sports Exerc. 1987;  19 491-496
    PubMedGoogle Scholar
  • 5 Buchanan KD, MacKiddie MT. The normal insulin response to glucose. The relationship between blood sugar and plasma insulin.  Diabetologia. 1967;  3 460-464
    CrossrefPubMedGoogle Scholar
  • 6 Burke LM, Collier GR, Hargreaves M. Muscle glycogen storage after prolonged exercise: Effect of the glycemic index of carbohydrate feedings.  J Appl Physiol. 1993;  75 1019-1023
    PubMedGoogle Scholar
  • 7 Carrithers JA, Williamson DL, Gallagher PM, Godard MP, Schulze KE, Trappe SW. Effects of postexercise carbohydrate-protein feedings on muscle glycogen restoration.  J Appl Physiol. 2000;  88 1976-1982
    PubMedGoogle Scholar
  • 8 Chan MH, MacGee SL, Watt MJ, Hargreaves M, Febbraio MA. Altering dietary nutrient intake that reduces glycogen content leads to phosphorylation of nuclear p38 map kinase in human skeletal muscle: Association with il-6 gene transcription during contraction.  FASEB J. 2004;  18 1785-1787
    PubMedGoogle Scholar
  • 9 Creer A, Gallagher P, Slivka D, Jemiolo B, Fink W, Trappe S. Influence of muscle glycogen availability on erk1/2 and akt signaling after resistance exercise in human skeletal muscle.  J Appl Physiol. 2005;  99 950-956
    CrossrefPubMedGoogle Scholar
  • 10 De Bock K. Exercise in the fasted state facilitates fibre-type specific intramyocellular lipid breakdown and stimulates glycogen resynthesis in humans.  J Physiol. 2005;  564 649-660
    CrossrefPubMedGoogle Scholar
  • 11 Fallowfield JL, Williams C. Carbohydrate intake and recovery from prolonged exercise.  Int J Sport Nutr. 1993;  3 150-164
    PubMedGoogle Scholar
  • 12 Foskett A, Williams C, Boobis L, Tsintzas K. Carbohydrate availability and muscle energy metabolism during intermittent running.  Med Sci Sports Exerc. 2008;  40 96-103
    PubMedGoogle Scholar
  • 13 Goodyear LJ, Hirshman MF, King PA, Horton ED, Thompson CM, Horton ES. Skeletal muscle plasma membrane glucose transport and glucose transporters after exercise.  J Appl Physiol. 1990;  68 193-198
    PubMedGoogle Scholar
  • 14 Hargreaves M, Cameron-Smith D. Exercise, diet, and skeletal muscle gene expression.  Med Sci Sports Exerc. 2002;  34 1505-1508
    CrossrefPubMedGoogle Scholar
  • 15 Ivy JL. Muscle glycogen synthesis before and after exercise.  Sports Med. 1991;  11 6-19
    CrossrefPubMedGoogle Scholar
  • 16 Ivy JL, Goforth  Jr  HW, Damon BM, MacCauley TR, Parsons EC, Price TB. Early postexercise muscle glycogen recovery is enhanced with a carbohydrate-protein supplement.  J Appl Physiol. 2002;  93 1337-1344
    PubMedGoogle Scholar
  • 17 Ivy JL, Holloszy JO. Persistent increase in glucose uptake by rat skeletal muscle following exercise.  Am J Physiol. 1981;  241 C200-C203
    PubMedGoogle Scholar
  • 18 Ivy JL, Katz AL, Cutler CL, Sherman WM, Coyle EF. Muscle glycogen synthesis after exercise: Effect of time of carbohydrate ingestion.  J Appl Physiol. 1988;  64 1480-1485
    CrossrefPubMedGoogle Scholar
  • 19 Ivy JL, Lee MC, Brozinick  Jr  JT, Reed MJ. Muscle glycogen storage after different amounts of carbohydrate ingestion.  J Appl Physiol. 1988;  65 2018-2023
    CrossrefPubMedGoogle Scholar
  • 20 James DE, Jenkins AB, Kraegen EW. Heterogeneity of insulin action in individual muscles in vivo: Euglycemic clamp studies in rats.  Am J Physiol. 1985;  248 E567-E574
    PubMedGoogle Scholar
  • 21 Jentjens R, Jeukendrup A. Determinants of post-exercise glycogen synthesis during short-term recovery.  Sports Med. 2003;  33 117-144
    CrossrefPubMedGoogle Scholar
  • 22 Nuttall FQ, Mooradian AD, Gannon MC, Billington C, Krezowski P. Effect of protein ingestion on the glucose and insulin response to a standardized oral glucose load.  Diabetes Care. 1984;  7 465-470
    PubMedGoogle Scholar
  • 23 Parkin JA, Carey MF, Martin IK, Stojanovska L, Febbraio MA. Muscle glycogen storage following prolonged exercise: Effect of timing of ingestion of high glycemic index food.  Med Sci Sports Exerc. 1997;  29 220-224
    PubMedGoogle Scholar
  • 24 Pilegaard H, Osada T, Andersen LT, Helge JW, Saltin B, Neufer PD. Substrate availability and transcriptional regulation of metabolic genes in human skeletal muscle during recovery from exercise.  Metabolism. 2005;  54 1048-1055
    CrossrefPubMedGoogle Scholar
  • 25 Rabinowitz D, Merimee TJ, Maffezzoli R, Burgess JA. Patterns of hormonal release after glucose, protein, and glucose plus protein.  Lancet. 1966;  2 454-456
    PubMedGoogle Scholar
  • 26 Rotman S, Slotboom J, Kreis R, Boesch C, Jequier E. Muscle glycogen recovery after exercise measured by 13c-magnetic resonance spectroscopy in humans: Effect of nutritional solutions.  MAGMA. 2000;  11 114-121
    PubMedGoogle Scholar
  • 27 Roy BD, Tarnopolsky MA. Influence of differing macronutrient intakes on muscle glycogen resynthesis after resistance exercise.  J Appl Physiol. 1998;  84 890-896
    PubMedGoogle Scholar
  • 28 Ruby BC, Gaskill SE, Slivka D, Harger SG. The addition of fenugreek extract (trigonella foenum-graecum) to glucose feeding increases muscle glycogen resynthesis after exercise.  Amino Acids. 2005;  28 71-76
    CrossrefPubMedGoogle Scholar
  • 29 Siri WE. Body composition from fluid spaces and density: Analysis of methods. Nutrition. 1993;  9 480-491
    PubMed
  • 30 Steinberg GR, Watt MJ, MacGee SL, Chan S, Hargreaves M, Febbraio MA, Stapleton D, Kemp BE. Reduced glycogen availability is associated with increased ampkalpha2 activity, nuclear ampkalpha2 protein abundance, and glut4 mrna expression in contracting human skeletal muscle.  Appl Physiol Nutr Metab. 2006;  31 302-312
    CrossrefPubMedGoogle Scholar
  • 31 Tarnopolsky MA, Bosman M, Macdonald JR, Vandeputte D, Martin J, Roy BD. Postexercise protein-carbohydrate and carbohydrate supplements increase muscle glycogen in men and women.  J Appl Physiol. 1997;  83 1877-1883
    PubMedGoogle Scholar
  • 32 Tunstall RJ, Cameron-Smith D. Effect of elevated lipid concentrations on human skeletal muscle gene expression.  Metabolism. 2005;  54 952-959
    CrossrefPubMedGoogle Scholar
  • 33 van Hall G, Saris WH, van de Schoor PA, Wagenmakers AJ. The effect of free glutamine and peptide ingestion on the rate of muscle glycogen resynthesis in man.  Int J Sports Med. 2000;  21 25-30
    Article in Thieme ConnectPubMedGoogle Scholar
  • 34 Loon LJ van, Saris WH, Kruijshoop M, Wagenmakers AJ. Maximizing postexercise muscle glycogen synthesis: Carbohydrate supplementation and the application of amino acid or protein hydrolysate mixtures.  Am J Clin Nutr. 2000;  72 106-111
    PubMedGoogle Scholar
  • 35 van Loon LJ, Saris WH, Verhagen H, Wagenmakers AJ. Plasma insulin responses after ingestion of different amino acid or protein mixtures with carbohydrate.  Am J Clin Nutr. 2000;  72 96-105
    PubMedGoogle Scholar
  • 36 Williams MB, Raven PB, Fogt DL, Ivy JL. Effects of recovery beverages on glycogen restoration and endurance exercise performance.  J Strength Cond Res. 2003;  17 12-19
    CrossrefPubMedGoogle Scholar
  • 37 Wojtaszewski JF, MacDonald C, Nielsen JN, Hellsten Y, Hardie DG, Kemp BE, Kiens B, Richter EA. Regulation of 5’amp-activated protein kinase activity and substrate utilization in exercising human skeletal muscle.  Am J Physiol. 2003;  284 E813-E822
    PubMedGoogle Scholar
  • 38 Zawadzki KM, Yaspelkis  3rd  BB, Ivy JL. Carbohydrate-protein complex increases the rate of muscle glycogen storage after exercise.  J Appl Physiol. 1992;  72 1854-1859
    PubMedGoogle Scholar

Correspondence

Dr. B. RubyPhD 

Montana Center for Work Physiology and Exercise Metabolism

The University of Montana

32 Campus Drive

59812 Missoula

United States

Phone: +406/243/21 17

Fax: +406/243/62 52

Email: Brent.ruby@mso.umt.edu

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