On the Blood-Brain Barrier to Peptides: [3H]Gonadotropin-Releasing Hormone Accumulation by Eighteen Regions of the Rat Brain and by Anterior Pituitary

A. Ermisch; H.-J. Rühle; E. Klauschenz; R. Kretzschmar

doi:10.1055/s-0029-1210374

Experimental and Clinical Endocrinology & Diabetes, Table of Contents

Exp Clin Endocrinol Diabetes 1984; 84(4): 112-116
DOI: 10.1055/s-0029-1210374

Original

On the Blood-Brain Barrier to Peptides: [³H]Gonadotropin-Releasing Hormone Accumulation by Eighteen Regions of the Rat Brain and by Anterior Pituitary

A. Ermisch¹ , H.-J. Rühle¹ , E. Klauschenz² , R. Kretzschmar²

¹Department of Cell Biology and Regulation (Head: Prof. Dr. A. Ermisch) of the Section of Biosciences (Head: Prof. Dr. G. Wagner), Karl Marx University Leipzig
²Institute of Drug Research of the Academy of Sciences of the GDR (Head: Prof. Dr. P. Oehme), Berlin

Abstract

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Summary

After intracarotid injection of [³H]gonadotropin-releasing hormone ([³H]GnRH) the mean accumulation of radioactivity per unit wet weight of 18 investigated brain samples and the anterior pituitary was 0.38 ± 0.11% g⁻¹ of the injected tracer dose. This indicates a low but measurable brain uptake of the peptide. The brain uptake of [³H]GnRH in blood-brain barrier (BBB)-protected regions is 5% of that of separately investigated [³H]OH. In BBB-free regions the accumulation of radioactivity was more than 25-fold higher than in BBB-protected regions. The accumulation of [³H]GnRH among regions with BBB varies less than among regions with leaky endothelia. The data presented for [³H]GnRH are similar to those for other peptides so far investigated.

Key words

GnRH - blood-brain barrier - rat

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